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mmu_circOrc5_659
Basic information of circular RNA
PanCircBase ID:
mmu_circOrc5_659
mm39_Chr. Position ID:
chr5|22704973|22731581|-
Gene name:
Orc5
Transcript:
ENSMUST00000135906.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
12,11,10,9,8,7
Exon/intron offsets:
0,16728,22415,23540,24840,26387
Genomic length:
26608
Exon/intron sizes in nt:
1,13,11,14,81,13,53,220
Flanking exon/introns:
chr5:22692215-22704973|chr5:22731581-22734152
Splice length:
659
Other database IDs:
circAtlas: mmu-Orc5_0002
circBase: hsa_circ_0081794, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_56135
Human Ortholog IDs:
Mature spliced Sequence:
GCAGTGCTTAATTTCCCCAAATACTGTGAACCTGTAGTTAAAGGAGAAGGTAACTGACTATCATTATTAATATTTCATGTGAGAGCATTTATACAAGATTTAAATTATTGAAATTCTCTCCTGGGAGTAGCAGGTGAACGTGATACTCGCAAACTGTGGAGAAATATTGAACCTCACCTGAAGAAGGCAATGCAGACTGTGTATCTCAGGGAGATCTCAAGTTCCCAGTGGGAAAAGTTACAGAAAGATAACACAGACCCAGGACAACTGAAAGGTCTCTCGGCGTACACCCACATGGAACTTCCATATTACTCCAAGTTTATTCTTATTGCTGCATACCTTGCCTCTTATAACCCAGCGAGAACTGATAAGAGGTTCTTCCTTAAGCATCATGGGAAAATCAAGAAGACTAACTTTCTAAAGAAGCATGAGAAGACAAGCAATCATCTTCTTGGACCAAAGCCCTTTCCACTGGACAGATTATTAGCAATATTGTATAGTATAGTGGACAGCAGAGTGGCTCCAACAGCAAATATCTTCTCCCAGATTACCTCCCTAGTGACTCTACAGCTGTTAACCATAGTTGGTCATGAAGATCAACTTAATGGGCCAAAATACAAATGCACAGTTTCTCTAGATTTCATCAGAGCTATTGCACG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGACTCTACAGCTGTTAACCA
56.33
TCTCCTTTAACTACAGGTTCACAGT
59.4
147
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGACTCTACAGCTGTTAACCA
56.33
ACCTTCTCCTTTAACTACAGGTTCA
59.39
151
PCR template sequence:
TGACTCTACAGCTGTTAACCATAGTTGGTCATGAAGATCAACTTAATGGGCCAAAATACAAATGCACAGTTTCTCTAGATTTCATCAGAGCTATTGCACGGCAGTGCTTAATTTCCCCAAATACTGTGAACCTGTAGTTAAAGGAGAAGGTAACTGACTATCATTATTAATATTTCATGTGAGAGCATTTATACAAGATT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-137-3p(1), mmu-miR-185-5p(1), mmu-miR-301a-3p(1), mmu-miR-5101(1), mmu-miR-3470b(1), mmu-miR-670-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GAGCTATTGCACGGCAGTGCTTAATT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCORC5_659_ORF_1
0.8298
0.014541227
0.496834282
507
ORF sequence:
ATGCAGACTGTGTATCTCAGGGAGATCTCAAGTTCCCAGTGGGAAAAGTTACAGAAAGATAACACAGACCCAGGACAACTGAAAGGTCTCTCGGCGTACACCCACATGGAACTTCCATATTACTCCAAGTTTATTCTTATTGCTGCATACCTTGCCTCTTATAACCCAGCGAGAACTGATAAGAGGTTCTTCCTTAAGCATCATGGGAAAATCAAGAAGACTAACTTTCTAAAGAAGCATGAGAAGACAAGCAATCATCTTCTTGGACCAAAGCCCTTTCCACTGGACAGATTATTAGCAATATTGTATAGTATAGTGGACAGCAGAGTGGCTCCAACAGCAAATATCTTCTCCCAGATTACCTCCCTAGTGACTCTACAGCTGTTAACCATAGTTGGTCATGAAGATCAACTTAATGGGCCAAAATACAAATGCACAGTTTCTCTAGATTTCATCAGAGCTATTGCACGGCAGTGCTTAATTTCCCCAAATACTGTGAACCTGTAG
Peptide sequence:
MQTVYLREISSSQWEKLQKDNTDPGQLKGLSAYTHMELPYYSKFILIAAYLASYNPARTDKRFFLKHHGKIKKTNFLKKHEKTSNHLLGPKPFPLDRLLAILYSIVDSRVAPTANIFSQITSLVTLQLLTIVGHEDQLNGPKYKCTVSLDFIRAIARQCLISPNTVNL
IRESfinder result
IRESfinder score
0.833861903
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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