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mmu_circNr2c2_195
Basic information of circular RNA
PanCircBase ID:
mmu_circNr2c2_195
mm39_Chr. Position ID:
chr6|92082262|92116871|+
Gene name:
Nr2c2
Transcript:
ENSMUST00000113463.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
2,3
Exon/intron offsets:
0,34498
Genomic length:
34609
Exon/intron sizes in nt:
84,111
Flanking exon/introns:
chr6:92069795-92082262|chr6:92116871-92126584
Splice length:
195
Other database IDs:
CIRCpedia: MMU_CIRCpedia_224498, circAtlas: mmu-Nr2c2_0015
Human Ortholog IDs:
Mature spliced Sequence:
GTTCTCTAATGATCTTGCTTCACCATGGCTACAAATATGGAGGGGCTGGTTCAGCACAGAGTGGGGACCCAGCAGGTGGCTGAGGTACCACGTACACAGACCTCTTGGCCGGAATCTCCAGGGATGACCAGCCCCTCCCCGCGCATCCAGATAATTTCCACCGACTCTGCGGTAGCCTCACCTCAGCGCATTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCCCGCGCATCCAGATAATT
60.61
GTGTACGTGGTACCTCAGCC
60.11
156
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTCTTGGCCGGAATCTCCAG
60.18
GTCCCCACTCTGTGCTGAAC
60.6
162
PCR template sequence:
GACCTCTTGGCCGGAATCTCCAGGGATGACCAGCCCCTCCCCGCGCATCCAGATAATTTCCACCGACTCTGCGGTAGCCTCACCTCAGCGCATTCAGGTTCTCTAATGATCTTGCTTCACCATGGCTACAAATATGGAGGGGCTGGTTCAGCACAGAGTGGGGACCCAGCAGGTGGCTGAGGTACCACGTACAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-370-3p(1), mmu-miR-3470b(1)
siRNAs for circular RNA silencing
Junction sequence
TCAGCGCATTCAGGTTCTCTAATGAT
siRNA sets
ATTAGAGAACCTGAATGCG, TAGAGAACCTGAATGCGCT, CATTAGAGAACCTGAATGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCNR2C2_195_ORF_1
1.0553
0.165001462
0.211452418
180
ORF sequence:
ATGGCTACAAATATGGAGGGGCTGGTTCAGCACAGAGTGGGGACCCAGCAGGTGGCTGAGGTACCACGTACACAGACCTCTTGGCCGGAATCTCCAGGGATGACCAGCCCCTCCCCGCGCATCCAGATAATTTCCACCGACTCTGCGGTAGCCTCACCTCAGCGCATTCAGGTTCTCTAA
Peptide sequence:
MATNMEGLVQHRVGTQQVAEVPRTQTSWPESPGMTSPSPRIQIISTDSAVASPQRIQVL
IRESfinder result
IRESfinder score
0.457530418
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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