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mmu_circNpepps_594
Basic information of circular RNA
PanCircBase ID:
mmu_circNpepps_594
mm39_Chr. Position ID:
chr11|97133428|97158505|-
Gene name:
Npepps
Transcript:
ENSMUST00000167806.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
6,5,4,3,2
Exon/intron offsets:
0,1825,5599,15671,24992
Genomic length:
25077
Exon/intron sizes in nt:
201,108,122,78,85
Flanking exon/introns:
chr11:97132873-97133428|chr11:97158505-97171026
Splice length:
594
Other database IDs:
circAtlas: mmu-Npepps_0077
Human Ortholog IDs:
Mature spliced Sequence:
GTAAGGCAGGCAACCAATCAAATTGTGATGAATTGTGCTGATATTGACATTATTACAGCTTCATATGCCCCAGAAGGAGATGAAGAAATCCATGCGACAGGATTTAACTATCAGAATGAAGATGAGAAAGTCACCTTGTCTTTTCCTAGCACTCTGCAAACAGGTACAGGAACCTTAAAGATAGATTTTGTTGGAGAGCTGAATGACAAAATGAAAGGTTTCTATAGAAGCAGATACACCACCCCTGCCGGCGAGGTGCGCTATGCTGCTGTCACACAGTTTGAGGCTACTGATGCCCGAAGGGCTTTTCCTTGCTGGGATGAGCCTGCTATCAAAGCAACTTTTGATATCTCGCTGGTTGTGCCTAAAGACAGAGTGGCTTTATCAAATATGAATGTAATTGACAGGAAACCATATCCTGATGATGAAAATTTAGTGGAAGTGAAGTTTGCTCGCACACCTGTTATGTCTACGTATCTGGTGGCATTTGTTGTGGGTGAATATGACTTTGTAGAAACAAGGTCAAAAGATGGTGTGTGTGTCCGTGTTTACACCCCTGTTGGCAAAGCAGAGCAAGGAAAGTTTGCGCTCGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGTCCGTGTTTACACCCCTG
59.89
CTTCATCTCCTTCTGGGGCA
59.09
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTGTCCGTGTTTACACCCCT
59.89
CTTCATCTCCTTCTGGGGCA
59.09
141
PCR template sequence:
GGGTGAATATGACTTTGTAGAAACAAGGTCAAAAGATGGTGTGTGTGTCCGTGTTTACACCCCTGTTGGCAAAGCAGAGCAAGGAAAGTTTGCGCTCGAGGTAAGGCAGGCAACCAATCAAATTGTGATGAATTGTGCTGATATTGACATTATTACAGCTTCATATGCCCCAGAAGGAGATGAAGAAATCCATGCGACAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-3065-5p(2), mmu-miR-103-3p(1), mmu-miR-30c-5p(1), mmu-miR-30b-5p(1), mmu-miR-107-3p(1), mmu-miR-335-3p(1), mmu-miR-484(1), mmu-miR-181d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GTTTGCGCTCGAGGTAAGGCAGGCAA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCNPEPPS_594_ORF_1
1.0982
0.165328077
0.997057358
1161
ORF sequence:
ATGAATTGTGCTGATATTGACATTATTACAGCTTCATATGCCCCAGAAGGAGATGAAGAAATCCATGCGACAGGATTTAACTATCAGAATGAAGATGAGAAAGTCACCTTGTCTTTTCCTAGCACTCTGCAAACAGGTACAGGAACCTTAAAGATAGATTTTGTTGGAGAGCTGAATGACAAAATGAAAGGTTTCTATAGAAGCAGATACACCACCCCTGCCGGCGAGGTGCGCTATGCTGCTGTCACACAGTTTGAGGCTACTGATGCCCGAAGGGCTTTTCCTTGCTGGGATGAGCCTGCTATCAAAGCAACTTTTGATATCTCGCTGGTTGTGCCTAAAGACAGAGTGGCTTTATCAAATATGAATGTAATTGACAGGAAACCATATCCTGATGATGAAAATTTAGTGGAAGTGAAGTTTGCTCGCACACCTGTTATGTCTACGTATCTGGTGGCATTTGTTGTGGGTGAATATGACTTTGTAGAAACAAGGTCAAAAGATGGTGTGTGTGTCCGTGTTTACACCCCTGTTGGCAAAGCAGAGCAAGGAAAGTTTGCGCTCGAGGTAAGGCAGGCAACCAATCAAATTGTGATGAATTGTGCTGATATTGACATTATTACAGCTTCATATGCCCCAGAAGGAGATGAAGAAATCCATGCGACAGGATTTAACTATCAGAATGAAGATGAGAAAGTCACCTTGTCTTTTCCTAGCACTCTGCAAACAGGTACAGGAACCTTAAAGATAGATTTTGTTGGAGAGCTGAATGACAAAATGAAAGGTTTCTATAGAAGCAGATACACCACCCCTGCCGGCGAGGTGCGCTATGCTGCTGTCACACAGTTTGAGGCTACTGATGCCCGAAGGGCTTTTCCTTGCTGGGATGAGCCTGCTATCAAAGCAACTTTTGATATCTCGCTGGTTGTGCCTAAAGACAGAGTGGCTTTATCAAATATGAATGTAATTGACAGGAAACCATATCCTGATGATGAAAATTTAGTGGAAGTGAAGTTTGCTCGCACACCTGTTATGTCTACGTATCTGGTGGCATTTGTTGTGGGTGAATATGACTTTGTAGAAACAAGGTCAAAAGATGGTGTGTGTGTCCGTGTTTACACCCCTGTTGGCAAAGCAGAGCAAGGAAAGTTTGCGCTCGAG
Peptide sequence:
MNCADIDIITASYAPEGDEEIHATGFNYQNEDEKVTLSFPSTLQTGTGTLKIDFVGELNDKMKGFYRSRYTTPAGEVRYAAVTQFEATDARRAFPCWDEPAIKATFDISLVVPKDRVALSNMNVIDRKPYPDDENLVEVKFARTPVMSTYLVAFVVGEYDFVETRSKDGVCVRVYTPVGKAEQGKFALEVRQATNQIVMNCADIDIITASYAPEGDEEIHATGFNYQNEDEKVTLSFPSTLQTGTGTLKIDFVGELNDKMKGFYRSRYTTPAGEVRYAAVTQFEATDARRAFPCWDEPAIKATFDISLVVPKDRVALSNMNVIDRKPYPDDENLVEVKFARTPVMSTYLVAFVVGEYDFVETRSKDGVCVRVYTPVGKAEQGKFALE
IRESfinder result
IRESfinder score
0.433039274
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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