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mmu_circNpepps_275
Basic information of circular RNA
PanCircBase ID:
mmu_circNpepps_275
mm39_Chr. Position ID:
chr11|97115484|97117694|-
Gene name:
Npepps
Transcript:
ENSMUST00000167806.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
16,15
Exon/intron offsets:
0,2070
Genomic length:
2210
Exon/intron sizes in nt:
1,35,140
Flanking exon/introns:
chr11:97104704-97115484|chr11:97117694-97120655
Splice length:
275
Other database IDs:
CIRCpedia: MMU_CIRCpedia_35542, circAtlas: mmu-Npepps_0065
Human Ortholog IDs:
Mature spliced Sequence:
GTGAAGACTGTCCTCAGTGGATGGTTCCTATCACAATTTCAACTAGTGAGGATCCTAACCAGGCTAAGCTGAAAATACTAATGGATAAGCCAGAGATGAGTGTGGTTTTGAAAAATGTCAAACCAGACCAATGGGTAAAGCTAAATCTGGGAACAGTTGGGTTTTATCGAACCCAGTACAGCTCTGCCATGCTCGAAAGTTTATTACCAGGCATCCGTGACCTTTCTCTGCCCCCAGTGGATCGACTTGGATTACAGAATGACCTCTTTTCTCTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAGGCATCCGTGACCTTTCT
60.04
AGTATTTTCAGCTTAGCCTGGT
57.42
147
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATTACCAGGCATCCGTGACC
59.82
AGTATTTTCAGCTTAGCCTGGT
57.42
152
PCR template sequence:
GTACAGCTCTGCCATGCTCGAAAGTTTATTACCAGGCATCCGTGACCTTTCTCTGCCCCCAGTGGATCGACTTGGATTACAGAATGACCTCTTTTCTCTGGTGAAGACTGTCCTCAGTGGATGGTTCCTATCACAATTTCAACTAGTGAGGATCCTAACCAGGCTAAGCTGAAAATACTAATGGATAAGCCAGAGATGAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-29b-1-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CCTCTTTTCTCTGGTGAAGACTGTCC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCNPEPPS_275_ORF_1
0.7718
0.030164008
0.207896925
327
ORF sequence:
ATGGTTCCTATCACAATTTCAACTAGTGAGGATCCTAACCAGGCTAAGCTGAAAATACTAATGGATAAGCCAGAGATGAGTGTGGTTTTGAAAAATGTCAAACCAGACCAATGGGTAAAGCTAAATCTGGGAACAGTTGGGTTTTATCGAACCCAGTACAGCTCTGCCATGCTCGAAAGTTTATTACCAGGCATCCGTGACCTTTCTCTGCCCCCAGTGGATCGACTTGGATTACAGAATGACCTCTTTTCTCTGGTGAAGACTGTCCTCAGTGGATGGTTCCTATCACAATTTCAACTAGTGAGGATCCTAACCAGGCTAAGCTGA
Peptide sequence:
MVPITISTSEDPNQAKLKILMDKPEMSVVLKNVKPDQWVKLNLGTVGFYRTQYSSAMLESLLPGIRDLSLPPVDRLGLQNDLFSLVKTVLSGWFLSQFQLVRILTRLS
IRESfinder result
IRESfinder score
0.676607415
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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