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mmu_circNfe2l2_267
Basic information of circular RNA
PanCircBase ID:
mmu_circNfe2l2_267
mm39_Chr. Position ID:
chr2|75509506|75509773|-
Gene name:
Nfe2l2
Transcript:
ENSMUST00000102672.5
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
2
Exon/intron offsets:
0
Genomic length:
267
Exon/intron sizes in nt:
267
Flanking exon/introns:
chr2:75508921-75509506|chr2:75509773-75534707
Splice length:
267
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GACATGGATTTGATTGACATCCTTTGGAGGCAAGACATAGATCTTGGAGTAAGTCGAGAAGTGTTTGACTTTAGTCAGCGACAGAAGGACTATGAGCTGGAAAAACAGAAAAAACTCGAAAAGGAAAGACAAGAGCAACTCCAGAAGGAACAGGAGAAGGCCTTTTTTGCTCAGTTTCAACTGGATGAAGAAACAGGAGAATTCCTCCCAATTCAGCCGGCCCAGCACATCCAGACAGACACCAGTGGATCCGCCAGCTACTCCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCACATCCAGACAGACACCA
59.96
GCTCATAGTCCTTCTGTCGCT
59.59
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCACATCCAGACAGACACCA
59.96
AGCTCATAGTCCTTCTGTCGC
59.59
141
PCR template sequence:
TGCTCAGTTTCAACTGGATGAAGAAACAGGAGAATTCCTCCCAATTCAGCCGGCCCAGCACATCCAGACAGACACCAGTGGATCCGCCAGCTACTCCCAGGACATGGATTTGATTGACATCCTTTGGAGGCAAGACATAGATCTTGGAGTAAGTCGAGAAGTGTTTGACTTTAGTCAGCGACAGAAGGACTATGAGCTGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-127-3p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
CAGCTACTCCCAGGACATGGATTTGA
siRNA sets
TCAAATCCATGTCCTGGGA, CATGTCCTGGGAGTAGCTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCNFE2L2_267_ORF_1
1.2562
0.326965142
0.90458024
531
ORF sequence:
ATGGATTTGATTGACATCCTTTGGAGGCAAGACATAGATCTTGGAGTAAGTCGAGAAGTGTTTGACTTTAGTCAGCGACAGAAGGACTATGAGCTGGAAAAACAGAAAAAACTCGAAAAGGAAAGACAAGAGCAACTCCAGAAGGAACAGGAGAAGGCCTTTTTTGCTCAGTTTCAACTGGATGAAGAAACAGGAGAATTCCTCCCAATTCAGCCGGCCCAGCACATCCAGACAGACACCAGTGGATCCGCCAGCTACTCCCAGGACATGGATTTGATTGACATCCTTTGGAGGCAAGACATAGATCTTGGAGTAAGTCGAGAAGTGTTTGACTTTAGTCAGCGACAGAAGGACTATGAGCTGGAAAAACAGAAAAAACTCGAAAAGGAAAGACAAGAGCAACTCCAGAAGGAACAGGAGAAGGCCTTTTTTGCTCAGTTTCAACTGGATGAAGAAACAGGAGAATTCCTCCCAATTCAGCCGGCCCAGCACATCCAGACAGACACCAGTGGATCCGCCAGCTACTCCCAG
Peptide sequence:
MDLIDILWRQDIDLGVSREVFDFSQRQKDYELEKQKKLEKERQEQLQKEQEKAFFAQFQLDEETGEFLPIQPAQHIQTDTSGSASYSQDMDLIDILWRQDIDLGVSREVFDFSQRQKDYELEKQKKLEKERQEQLQKEQEKAFFAQFQLDEETGEFLPIQPAQHIQTDTSGSASYSQ
IRESfinder result
IRESfinder score
0.642852295
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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