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mmu_circNfe2l1_216
Basic information of circular RNA
PanCircBase ID:
mmu_circNfe2l1_216
mm39_Chr. Position ID:
chr11|96712929|96713145|-
Gene name:
Nfe2l1
Transcript:
ENSMUST00000167110.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
2
Exon/intron offsets:
0
Genomic length:
216
Exon/intron sizes in nt:
216
Flanking exon/introns:
chr11:96712235-96712929|chr11:96713145-96714770
Splice length:
216
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GATATAGATCTGATTGACATCCTTTGGCGACAGGATATTGATCTGGGGGCTGGGCGTGAGGTTTTTGACTACAGTCATCGCCAGAAGGAGCAGGATGTGGATAAGGAACTGCAAGATGGACGAGAACGAGAGGACACCTGGTCAGGCGAGGGTGCGGAAGCTCTGGCCCGAGACCTGCTAGTAGATGGAGAGACTGGGGAGAGCTTCCCTGCACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GACGAGAACGAGAGGACACC
59.83
AGTCAAAAACCTCACGCCCA
60.11
168
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGACGAGAACGAGAGGACAC
59.83
AGTCAAAAACCTCACGCCCA
60.11
169
PCR template sequence:
TGGACGAGAACGAGAGGACACCTGGTCAGGCGAGGGTGCGGAAGCTCTGGCCCGAGACCTGCTAGTAGATGGAGAGACTGGGGAGAGCTTCCCTGCACAGGATATAGATCTGATTGACATCCTTTGGCGACAGGATATTGATCTGGGGGCTGGGCGTGAGGTTTTTGACTACAGTCATCGCCAGAAGGAGCAGGATGTGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-452-3p(1), mmu-miR-697(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
CTTCCCTGCACAGGATATAGATCTGA
siRNA sets
TCAGATCTATATCCTGTGC, GATCTATATCCTGTGCAGG, CAGATCTATATCCTGTGCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.351997289
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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