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mmu_circNfat5_1005
Basic information of circular RNA
PanCircBase ID:
mmu_circNfat5_1005
mm39_Chr. Position ID:
chr8|108064926|108074489|+
Gene name:
Nfat5
Transcript:
ENSMUST00000154474.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
3,4,5,6
Exon/intron offsets:
0,514,6491,9372
Genomic length:
9563
Exon/intron sizes in nt:
62,55,91,93,191
Flanking exon/introns:
chr8:108051396-108064926|chr8:108074489-108078058
Splice length:
1005
Other database IDs:
CIRCpedia: HSA_CIRCpedia_368771
Human Ortholog IDs:
Mature spliced Sequence:
GATTTGCCTCTGAAGCAGGGAGTGTCTGCATTAAAAATGACCTGTAGTTCTCTGCTTCATAGATGCTTCTTCAGCTCCCTCCTCTTCCTCCATGGGCGGTGCTTGCAGCTCCTTTACCACCTCTTCCAGCCCTACCATTTATTCTACCTCAGTCACCGACAGCAAGGCTATGCAAGTGGAGAGCTGCTCCTCAGCCGTGGGGGTAAGTAACAGAGGGGTAAGTGAAAAGCAGTTAACCGGTAACACAGTTCAGCAGCATCCATCAACCCCGAAGAGGCACACAGTTTTGTACATCTCACCACCACCTGAGGACTTGCTGGATAACAGTCGGATGTCCTGCCAGGATGAGGGGTGTGGATTGGAATCTGAGCAGAGCTGCAGTATGTGGATGGAGGATTCCCCCTCCAACTTCAGTAACATGAGCACCAGTTCCTACAATGATAACACTGAGGTACCTCGTAAATCACGAAAACGAAATCCAAAGCAGAGGCCGGGGGTCAAACGACGAGATTGTGAAGAATCTAATATGGATATATTTGATGCCGACAGTGCCAAAGCACCTCACTATGTGCTTTCTCAGCTTACCACGGACAACAAAGGCAACTCAAAAGCTGGAAATGGAACATTGGACAGCCAAAAGGGAACTGGAGTAAAGAAGAGCCCTATGTTGTGCGGACAGTATCCGGTTAAAAGTGAGGGGAAGGAGCTGAAGATAGTGGTACAGCCTGAAACCCAACACCGAGCCCGGTACCTGACAGAGGGCAGCCGAGGCTCTGTAAAAGACAGAACACAGCAAGGCTTTCCTACGGTGAAGCTGGAAGGCCATAATGAACCAGTGGTGTTGCAGGTATTTGTGGGCAATGATTCTGGTCGAGTAAAACCACATGGATTCTATCAGGCCTGTAGAGTAACTGGGCGAAATACAACTCCCTGCAAAGAAGTGGACATTGAAGGTACCACTGTTATAGAAGTTGGTCTTGATCCTAGCAACAACATGACACTGGC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCCCTGCAAAGAAGTGGACA
59.16
CCATGGAGGAAGAGGAGGGA
60.03
173
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCCCTGCAAAGAAGTGGACA
59.16
GAGGAAGAGGAGGGAGCTGA
60.03
168
PCR template sequence:
GAGTAACTGGGCGAAATACAACTCCCTGCAAAGAAGTGGACATTGAAGGTACCACTGTTATAGAAGTTGGTCTTGATCCTAGCAACAACATGACACTGGCGATTTGCCTCTGAAGCAGGGAGTGTCTGCATTAAAAATGACCTGTAGTTCTCTGCTTCATAGATGCTTCTTCAGCTCCCTCCTCTTCCTCCATGGGCGGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-134-5p(1), mmu-let-7a-5p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-miR-26a-5p(1), mmu-let-7c-5p(1), mmu-miR-204-5p(1), mmu-miR-98-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ACATGACACTGGCGATTTGCCTCTGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCNFAT5_1005_ORF_1
0.6979
0.201562332
0.971289629
927
ORF sequence:
ATGGGCGGTGCTTGCAGCTCCTTTACCACCTCTTCCAGCCCTACCATTTATTCTACCTCAGTCACCGACAGCAAGGCTATGCAAGTGGAGAGCTGCTCCTCAGCCGTGGGGGTAAGTAACAGAGGGGTAAGTGAAAAGCAGTTAACCGGTAACACAGTTCAGCAGCATCCATCAACCCCGAAGAGGCACACAGTTTTGTACATCTCACCACCACCTGAGGACTTGCTGGATAACAGTCGGATGTCCTGCCAGGATGAGGGGTGTGGATTGGAATCTGAGCAGAGCTGCAGTATGTGGATGGAGGATTCCCCCTCCAACTTCAGTAACATGAGCACCAGTTCCTACAATGATAACACTGAGGTACCTCGTAAATCACGAAAACGAAATCCAAAGCAGAGGCCGGGGGTCAAACGACGAGATTGTGAAGAATCTAATATGGATATATTTGATGCCGACAGTGCCAAAGCACCTCACTATGTGCTTTCTCAGCTTACCACGGACAACAAAGGCAACTCAAAAGCTGGAAATGGAACATTGGACAGCCAAAAGGGAACTGGAGTAAAGAAGAGCCCTATGTTGTGCGGACAGTATCCGGTTAAAAGTGAGGGGAAGGAGCTGAAGATAGTGGTACAGCCTGAAACCCAACACCGAGCCCGGTACCTGACAGAGGGCAGCCGAGGCTCTGTAAAAGACAGAACACAGCAAGGCTTTCCTACGGTGAAGCTGGAAGGCCATAATGAACCAGTGGTGTTGCAGGTATTTGTGGGCAATGATTCTGGTCGAGTAAAACCACATGGATTCTATCAGGCCTGTAGAGTAACTGGGCGAAATACAACTCCCTGCAAAGAAGTGGACATTGAAGGTACCACTGTTATAGAAGTTGGTCTTGATCCTAGCAACAACATGACACTGGCGATTTGCCTCTGA
Peptide sequence:
MGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTGNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLDSQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAICL
IRESfinder result
IRESfinder score
0.503391915
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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