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mmu_circNf2_741
Basic information of circular RNA
PanCircBase ID:
mmu_circNf2_741
mm39_Chr. Position ID:
chr11|4732186|4741213|-
Gene name:
Nf2
Transcript:
ENSMUST00000109910.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
15,14,13,12,11
Exon/intron offsets:
0,2249,5257,7478,8904
Genomic length:
9027
Exon/intron sizes in nt:
16,61,28,10,62,18,123
Flanking exon/introns:
chr11:4718273-4732186|chr11:4741213-4744411
Splice length:
741
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATGGAAAGGCAGCGGCTGGCTCGAGAGAAGCAGATGCGGGAGGAGGCCGAGCGTACAAGAGATGAGTTAGAGAGGAGGCTCCTGCAGATGAAAGAAGAAGCAACGATGGCCAATGAAGCTCTGATGCGCTCTGAGGAGACAGCTGATCTGTTGGCTGAAAAGGCTCAGATCACAGAGGAGGAGGCCAAGCTTTTGGCACAAAAGGCTGCAGAGGCTGAGCAAGAGATGCAGCGAATCAAGGCCACGGCCATTCGGACAGAGGAGGAGAAGCGCCTGATGGAGCAGAAGGTGCTGGAGGCTGAAGTGCTGGCATTGAAGATGGCTGAGGAGTCAGAGAGGAGGGCCAAGGAGGCTGATCAGTTAAAGCAAGACTTGCAAGAAGCCAGAGAAGCAGAGCGAAGAGCCAAGCAGAAGCTCTTAGAAATCGCCACCAAGCCCACCTATCCACCCATGAACCCAATTCCACCACCACTGCCTCCTGACATACCGAGCTTCGACATTATTGCTGACAGCTTGTCATTTGACTTCAAGGATACGGACATGAAGCGACTTTCCATGGAGATAGAGAAAGAAAAAGTGGAGTACATGGAGAAGAGCAAGCACCTGCAGGAGCAGCTCAACGAGCTCAAGACGGAGATCGAGGCCTTGAAACTCAAAGAGCGGGAGACGGCCTTGGACGTCCTACACAGCGAGAGCTCAGACAGAGGCGGCCCCAGCAGCAAGCATAATACCATTAAAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GACGTCCTACACAGCGAGAG
59.9
CTGCAGGAGCCTCCTCTCTA
60.11
153
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GACGTCCTACACAGCGAGAG
59.9
TTTCATCTGCAGGAGCCTCC
59.75
159
PCR template sequence:
GGCCTTGAAACTCAAAGAGCGGGAGACGGCCTTGGACGTCCTACACAGCGAGAGCTCAGACAGAGGCGGCCCCAGCAGCAAGCATAATACCATTAAAAAGATGGAAAGGCAGCGGCTGGCTCGAGAGAAGCAGATGCGGGAGGAGGCCGAGCGTACAAGAGATGAGTTAGAGAGGAGGCTCCTGCAGATGAAAGAAGAAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-207(2), mmu-miR-1904(2), mmu-miR-328-3p(1), mmu-miR-676-3p(1), mmu-miR-362-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TACCATTAAAAAGATGGAAAGGCAGC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCNF2_741_ORF_1
1.1428
0.591478586
0.999952765
1482
ORF sequence:
ATGGAAAGGCAGCGGCTGGCTCGAGAGAAGCAGATGCGGGAGGAGGCCGAGCGTACAAGAGATGAGTTAGAGAGGAGGCTCCTGCAGATGAAAGAAGAAGCAACGATGGCCAATGAAGCTCTGATGCGCTCTGAGGAGACAGCTGATCTGTTGGCTGAAAAGGCTCAGATCACAGAGGAGGAGGCCAAGCTTTTGGCACAAAAGGCTGCAGAGGCTGAGCAAGAGATGCAGCGAATCAAGGCCACGGCCATTCGGACAGAGGAGGAGAAGCGCCTGATGGAGCAGAAGGTGCTGGAGGCTGAAGTGCTGGCATTGAAGATGGCTGAGGAGTCAGAGAGGAGGGCCAAGGAGGCTGATCAGTTAAAGCAAGACTTGCAAGAAGCCAGAGAAGCAGAGCGAAGAGCCAAGCAGAAGCTCTTAGAAATCGCCACCAAGCCCACCTATCCACCCATGAACCCAATTCCACCACCACTGCCTCCTGACATACCGAGCTTCGACATTATTGCTGACAGCTTGTCATTTGACTTCAAGGATACGGACATGAAGCGACTTTCCATGGAGATAGAGAAAGAAAAAGTGGAGTACATGGAGAAGAGCAAGCACCTGCAGGAGCAGCTCAACGAGCTCAAGACGGAGATCGAGGCCTTGAAACTCAAAGAGCGGGAGACGGCCTTGGACGTCCTACACAGCGAGAGCTCAGACAGAGGCGGCCCCAGCAGCAAGCATAATACCATTAAAAAGATGGAAAGGCAGCGGCTGGCTCGAGAGAAGCAGATGCGGGAGGAGGCCGAGCGTACAAGAGATGAGTTAGAGAGGAGGCTCCTGCAGATGAAAGAAGAAGCAACGATGGCCAATGAAGCTCTGATGCGCTCTGAGGAGACAGCTGATCTGTTGGCTGAAAAGGCTCAGATCACAGAGGAGGAGGCCAAGCTTTTGGCACAAAAGGCTGCAGAGGCTGAGCAAGAGATGCAGCGAATCAAGGCCACGGCCATTCGGACAGAGGAGGAGAAGCGCCTGATGGAGCAGAAGGTGCTGGAGGCTGAAGTGCTGGCATTGAAGATGGCTGAGGAGTCAGAGAGGAGGGCCAAGGAGGCTGATCAGTTAAAGCAAGACTTGCAAGAAGCCAGAGAAGCAGAGCGAAGAGCCAAGCAGAAGCTCTTAGAAATCGCCACCAAGCCCACCTATCCACCCATGAACCCAATTCCACCACCACTGCCTCCTGACATACCGAGCTTCGACATTATTGCTGACAGCTTGTCATTTGACTTCAAGGATACGGACATGAAGCGACTTTCCATGGAGATAGAGAAAGAAAAAGTGGAGTACATGGAGAAGAGCAAGCACCTGCAGGAGCAGCTCAACGAGCTCAAGACGGAGATCGAGGCCTTGAAACTCAAAGAGCGGGAGACGGCCTTGGACGTCCTACACAGCGAGAGCTCAGACAGAGGCGGCCCCAGCAGCAAGCATAATACCATTAAAAAG
Peptide sequence:
MERQRLAREKQMREEAERTRDELERRLLQMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPPMNPIPPPLPPDIPSFDIIADSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDVLHSESSDRGGPSSKHNTIKKMERQRLAREKQMREEAERTRDELERRLLQMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPPMNPIPPPLPPDIPSFDIIADSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDVLHSESSDRGGPSSKHNTIKK
IRESfinder result
IRESfinder score
0.183153426
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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