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mmu_circNcor2_1634
Basic information of circular RNA
PanCircBase ID:
mmu_circNcor2_1634
mm39_Chr. Position ID:
chr5|125156071|125230716|-
Gene name:
Ncor2
Transcript:
ENSMUST00000111398.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
14
Exon/intron indexes:
15,14,13,12,11,10,9,8,7,6,5,4,3,2
Exon/intron offsets:
0,2122,5873,7833,8786,12646,18939,23635,27191,30885,39609,40474,45762,74599
Genomic length:
74645
Exon/intron sizes in nt:
99,55,179,94,173,67,53,57,114,180,178,128,211,46
Flanking exon/introns:
chr5:125146141-125156071|chr5:125230716-125256060
Splice length:
1634
Other database IDs:
CIRCpedia: MMU_CIRCpedia_6431, circAtlas: mmu-Ncor2_0042
Human Ortholog IDs:
Mature spliced Sequence:
TGCTCCTGAGTCTTTGAGGAACACAGCCTCCTGGTGGAAGTTCGTGGCACCTGTGACGAGGTCACCTGCCAGCAGATGACCGAGACCAGCCCTTAGTCCTAGGTGTGGTCAAGAGTGTCTTGGGCTCCAAAGCCTACCTGGACCCTACCACCATGTCAGGATCCACACAGCCTGTGGCACAGACATGGCGGGCTGCTGAGCCCCGCTACCCACCCCATGGCATCTCCTACCCGGTGCAGATAGCCCGGTCCCACACGGACGTGGGGCTGCTTGAGTACCAACACCACCCCCGTGACTACACCTCACACCTGTCACCCGGTTCCATCATCCAGCCACAGAGGAGGCGGCCCTCACTGCTGTCAGAGTTCCAGCCTGGGAGTGAACGGTCTCAGGAGCTCCACCTGCGCCCTGAGTCCCGCACGTTCCTGCCTGAGCTGGGCAAGCCCGACATAGAATTCACCGAGAGCAAGCGCCCCCGCCTGGAGCTACTACCCGATACCCTGCTGCGCCCATCACCCCTGCTGGCCACTGGGCAGCCGAGTGGGTCTGAAGACCTTACCAAGGACCGTAGCCTGGCAGGCAAGCTGGAGCCTGTGTCACCTCCCAGTCCCCCGCACGCTGACCCTGAGCTAGAGCTGGCGCCATCTCGACTGTCCAAGGAGGAGCTGATCCAGAACATGGACCGCGTGGACCGTGAGATCACCATGGTAGAGCAGCAGATCTCCAAGCTGAAGAAGAAGCAGCAACAGTTGGAGGAGGAGGCCGCCAAGCCGCCCGAACCCGAGAAGCCTGTGTCGCCACCACCCATAGAATCAAAGCACCGAAGCCTGGTCCAGATCATCTACGATGAGAACCGGAAGAAAGCCGAAGCCGCACACCGGATCCTAGAAGGCCTGGGGCCCCAGGTGGAGCTGCCTCTGTACAACCAGCCGTCTGACACACGCCAGTACCATGAAAACATCAAAATAAACCAGGCGATGCGGAAGAAGCTGATCTTGTACTTTAAGCGGAGGAACCACGCGCGCAAGCAGTGGGAACAGCGCTTCTGCCAGCGCTATGACCAGCTCATGGAGGCGTGGGAGAAGAAGGTAGAGCGCATAGAGAACAATCCGCGAAGGAGGGCCAAGGAGAGCAAGGTGAGGGAGTACTACGAGAAACAGTTCCCGGAGATCCGCAAGCAGCGGGAGCTGCAGGAGCGCATGCAGAGCAGGGTGGGCCAGCGTGGCAGTGGGCTCTCCATGTCGGCTGCCCGCAGTGAGCATGAGGTTTCTGAGATCATTGATGGCTTGTCTGAGCAGGAGAATCTGGAGAAGCAGATGCGCCAGCTGGCCGTGATCCCGCCCATGTTGTACGACGCGGACCAGCAGAGGATCAAGTTCATCAACATGAATGGACTCATGGATGACCCCATGAAGGTCTACAAGGACCGTCAGGTTACCAACATGTGGAGCGAGCAGGAGAGGGACACCTTCCGTGAGAAGTTTATGCAGCACCCTAAGAACTTTGGCCTGATTGCCTCATTCCTGGAGAGAAAGACGGTCGCTGAGTGTGTCCTCTATTACTACCTGACCAAGAAGAATGAAAATTACAAGAGCTTGGTGAGGCGGAGCTATCGGCGCCGTGGCAAGAGCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TACAAGAGCTTGGTGAGGCG
60.04
TAAGGGCTGGTCTCGGTCAT
60.33
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TACAAGAGCTTGGTGAGGCG
60.04
AGGACTAAGGGCTGGTCTCG
60.68
148
PCR template sequence:
GACGGTCGCTGAGTGTGTCCTCTATTACTACCTGACCAAGAAGAATGAAAATTACAAGAGCTTGGTGAGGCGGAGCTATCGGCGCCGTGGCAAGAGCCAGTGCTCCTGAGTCTTTGAGGAACACAGCCTCCTGGTGGAAGTTCGTGGCACCTGTGACGAGGTCACCTGCCAGCAGATGACCGAGACCAGCCCTTAGTCCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-205-5p(1), mmu-miR-328-3p(1), mmu-miR-24-3p(1), mmu-miR-136-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-666-5p(1), mmu-miR-705(1), mmu-miR-27a-5p(1), mmu-miR-207(1), mmu-miR-335-3p(1), mmu-miR-484(1)
siRNAs for circular RNA silencing
Junction sequence
TGGCAAGAGCCAGTGCTCCTGAGTCT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCNCOR2_1634_ORF_1
1.2606
0.559230351
0.999957704
1491
ORF sequence:
ATGTCAGGATCCACACAGCCTGTGGCACAGACATGGCGGGCTGCTGAGCCCCGCTACCCACCCCATGGCATCTCCTACCCGGTGCAGATAGCCCGGTCCCACACGGACGTGGGGCTGCTTGAGTACCAACACCACCCCCGTGACTACACCTCACACCTGTCACCCGGTTCCATCATCCAGCCACAGAGGAGGCGGCCCTCACTGCTGTCAGAGTTCCAGCCTGGGAGTGAACGGTCTCAGGAGCTCCACCTGCGCCCTGAGTCCCGCACGTTCCTGCCTGAGCTGGGCAAGCCCGACATAGAATTCACCGAGAGCAAGCGCCCCCGCCTGGAGCTACTACCCGATACCCTGCTGCGCCCATCACCCCTGCTGGCCACTGGGCAGCCGAGTGGGTCTGAAGACCTTACCAAGGACCGTAGCCTGGCAGGCAAGCTGGAGCCTGTGTCACCTCCCAGTCCCCCGCACGCTGACCCTGAGCTAGAGCTGGCGCCATCTCGACTGTCCAAGGAGGAGCTGATCCAGAACATGGACCGCGTGGACCGTGAGATCACCATGGTAGAGCAGCAGATCTCCAAGCTGAAGAAGAAGCAGCAACAGTTGGAGGAGGAGGCCGCCAAGCCGCCCGAACCCGAGAAGCCTGTGTCGCCACCACCCATAGAATCAAAGCACCGAAGCCTGGTCCAGATCATCTACGATGAGAACCGGAAGAAAGCCGAAGCCGCACACCGGATCCTAGAAGGCCTGGGGCCCCAGGTGGAGCTGCCTCTGTACAACCAGCCGTCTGACACACGCCAGTACCATGAAAACATCAAAATAAACCAGGCGATGCGGAAGAAGCTGATCTTGTACTTTAAGCGGAGGAACCACGCGCGCAAGCAGTGGGAACAGCGCTTCTGCCAGCGCTATGACCAGCTCATGGAGGCGTGGGAGAAGAAGGTAGAGCGCATAGAGAACAATCCGCGAAGGAGGGCCAAGGAGAGCAAGGTGAGGGAGTACTACGAGAAACAGTTCCCGGAGATCCGCAAGCAGCGGGAGCTGCAGGAGCGCATGCAGAGCAGGGTGGGCCAGCGTGGCAGTGGGCTCTCCATGTCGGCTGCCCGCAGTGAGCATGAGGTTTCTGAGATCATTGATGGCTTGTCTGAGCAGGAGAATCTGGAGAAGCAGATGCGCCAGCTGGCCGTGATCCCGCCCATGTTGTACGACGCGGACCAGCAGAGGATCAAGTTCATCAACATGAATGGACTCATGGATGACCCCATGAAGGTCTACAAGGACCGTCAGGTTACCAACATGTGGAGCGAGCAGGAGAGGGACACCTTCCGTGAGAAGTTTATGCAGCACCCTAAGAACTTTGGCCTGATTGCCTCATTCCTGGAGAGAAAGACGGTCGCTGAGTGTGTCCTCTATTACTACCTGACCAAGAAGAATGAAAATTACAAGAGCTTGGTGAGGCGGAGCTATCGGCGCCGTGGCAAGAGCCAGTGCTCCTGA
Peptide sequence:
MSGSTQPVAQTWRAAEPRYPPHGISYPVQIARSHTDVGLLEYQHHPRDYTSHLSPGSIIQPQRRRPSLLSEFQPGSERSQELHLRPESRTFLPELGKPDIEFTESKRPRLELLPDTLLRPSPLLATGQPSGSEDLTKDRSLAGKLEPVSPPSPPHADPELELAPSRLSKEELIQNMDRVDREITMVEQQISKLKKKQQQLEEEAAKPPEPEKPVSPPPIESKHRSLVQIIYDENRKKAEAAHRILEGLGPQVELPLYNQPSDTRQYHENIKINQAMRKKLILYFKRRNHARKQWEQRFCQRYDQLMEAWEKKVERIENNPRRRAKESKVREYYEKQFPEIRKQRELQERMQSRVGQRGSGLSMSAARSEHEVSEIIDGLSEQENLEKQMRQLAVIPPMLYDADQQRIKFINMNGLMDDPMKVYKDRQVTNMWSEQERDTFREKFMQHPKNFGLIASFLERKTVAECVLYYYLTKKNENYKSLVRRSYRRRGKSQCS
IRESfinder result
IRESfinder score
0.288771349
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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