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mmu_circNbeal1_464
Basic information of circular RNA
PanCircBase ID:
mmu_circNbeal1_464
mm39_Chr. Position ID:
chr1|60233662|60240150|+
Gene name:
Nbeal1
Transcript:
ENSMUST00000160834.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
3,4,5,6
Exon/intron offsets:
0,511,5601,6360
Genomic length:
6488
Exon/intron sizes in nt:
9,21,62,82,128
Flanking exon/introns:
chr1:60220913-60233662|chr1:60240150-60245492
Splice length:
464
Other database IDs:
CIRCpedia: MMU_CIRCpedia_217945, circAtlas: mmu-Nbeal1_0002
circBase: hsa_circ_0118753, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_39252
Human Ortholog IDs:
Mature spliced Sequence:
AAAGATCCAGATTACCTGAGACTGTGGTTGGACAATTTTGTTTTAAGTTATGAACAATTTTTAGATGTTGACTTTGAAAAGCTGCCTACAAGGGTGGATGATATGCCTCCAGGAATATCTCTGCTTCCTGATAATATTCTGCAGGTTCTGAGGATCCAGCTGCTACACTGTGTTCAGAAAACAGCAGATGGGTTAGAGGAACAACAACAAGCATTGTCAATTTTGCTTGTTAAATTCTTCATTATTCTTTGCAGGAATTTATCAAATGTAGAAGAAATTGGGACTTGCTCTTATATTAATCACGTCATTACCATGACAACACTGTATATTCAGCAATTAAAAAGCAAAAAAAAGGAGAAGGAAATGGCTGATCAGACATCTATTGAAGAATTTGTGATCCATGCACTGGCATTCTGTGAAAGCTTGTATGATCCATATAGAAATTGGAGACATAGAATTTCAGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTTGTGATCCATGCACTGGC
59.11
CCACCCTTGTAGGCAGCTTT
60.25
171
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTTGTGATCCATGCACTGGC
59.11
CATCCACCCTTGTAGGCAGC
60.75
174
PCR template sequence:
TGGCTGATCAGACATCTATTGAAGAATTTGTGATCCATGCACTGGCATTCTGTGAAAGCTTGTATGATCCATATAGAAATTGGAGACATAGAATTTCAGGAAAGATCCAGATTACCTGAGACTGTGGTTGGACAATTTTGTTTTAAGTTATGAACAATTTTTAGATGTTGACTTTGAAAAGCTGCCTACAAGGGTGGATG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-152-3p(1), mmu-miR-148b-3p(1), mmu-miR-128-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ATAGAATTTCAGGAAAGATCCAGATT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCNBEAL1_464_ORF_1
0.8057
-0.076890827
0.208919803
381
ORF sequence:
ATGCCTCCAGGAATATCTCTGCTTCCTGATAATATTCTGCAGGTTCTGAGGATCCAGCTGCTACACTGTGTTCAGAAAACAGCAGATGGGTTAGAGGAACAACAACAAGCATTGTCAATTTTGCTTGTTAAATTCTTCATTATTCTTTGCAGGAATTTATCAAATGTAGAAGAAATTGGGACTTGCTCTTATATTAATCACGTCATTACCATGACAACACTGTATATTCAGCAATTAAAAAGCAAAAAAAAGGAGAAGGAAATGGCTGATCAGACATCTATTGAAGAATTTGTGATCCATGCACTGGCATTCTGTGAAAGCTTGTATGATCCATATAGAAATTGGAGACATAGAATTTCAGGAAAGATCCAGATTACCTGA
Peptide sequence:
MPPGISLLPDNILQVLRIQLLHCVQKTADGLEEQQQALSILLVKFFIILCRNLSNVEEIGTCSYINHVITMTTLYIQQLKSKKKEKEMADQTSIEEFVIHALAFCESLYDPYRNWRHRISGKIQIT
IRESfinder result
IRESfinder score
0.762500372
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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