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mmu_circNbeal1_384
Basic information of circular RNA
PanCircBase ID:
mmu_circNbeal1_384
mm39_Chr. Position ID:
chr1|60220621|60233754|+
Gene name:
Nbeal1
Transcript:
ENSMUST00000160834.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
2,3
Exon/intron offsets:
0,13041
Genomic length:
13133
Exon/intron sizes in nt:
292,92
Flanking exon/introns:
chr1:60219844-60220621|chr1:60233754-60234173
Splice length:
384
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ACCTATTTACGAGACCAGCTACCCCTGATGAAGAAGTGTTGGAGTAGTTGCTGAAATTGACCCTTATTGACCTGAGACAGTCTTATTTGTTCTACGAATTTTTTTTTTTGTTTTTTTTTTGTTTATTCCACTGCTTTGAGCTTTACTGTAACGGCTGATAAACTTGGAAAATAAAGTAATATGATATGGTCTTGAACCGTCTTTTTGAGGAAATATTAAAGTACCAGAGTGGAAAGCCAGAATGGCAGCCAGAGAGAGGCTCTTTGAACTTTGGATGCTTTATTGTACAAAGAAAGATCCAGATTACCTGAGACTGTGGTTGGACAATTTTGTTTTAAGTTATGAACAATTTTTAGATGTTGACTTTGAAAAGCTGCCTACAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCTGAGACTGTGGTTGGACA
59.24
GGGTCAATTTCAGCAACTACTCC
59.56
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACCTGAGACTGTGGTTGGAC
59.24
GGGTCAATTTCAGCAACTACTCC
59.56
142
PCR template sequence:
GTACAAAGAAAGATCCAGATTACCTGAGACTGTGGTTGGACAATTTTGTTTTAAGTTATGAACAATTTTTAGATGTTGACTTTGAAAAGCTGCCTACAAGACCTATTTACGAGACCAGCTACCCCTGATGAAGAAGTGTTGGAGTAGTTGCTGAAATTGACCCTTATTGACCTGAGACAGTCTTATTTGTTCTACGAATT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-376a-3p(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AGCTGCCTACAAGACCTATTTACGAG
siRNA sets
TAAATAGGTCTTGTAGGCA, AAATAGGTCTTGTAGGCAG, AATAGGTCTTGTAGGCAGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCNBEAL1_384_ORF_1
0.439
-0.053756963
0.031355099
171
ORF sequence:
ATGGCAGCCAGAGAGAGGCTCTTTGAACTTTGGATGCTTTATTGTACAAAGAAAGATCCAGATTACCTGAGACTGTGGTTGGACAATTTTGTTTTAAGTTATGAACAATTTTTAGATGTTGACTTTGAAAAGCTGCCTACAAGACCTATTTACGAGACCAGCTACCCCTGA
Peptide sequence:
MAARERLFELWMLYCTKKDPDYLRLWLDNFVLSYEQFLDVDFEKLPTRPIYETSYP
IRESfinder result
IRESfinder score
0.912961295
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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