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mmu_circNbeal1_2520
Basic information of circular RNA
PanCircBase ID:
mmu_circNbeal1_2520
mm39_Chr. Position ID:
chr1|60296588|60318959|+
Gene name:
Nbeal1
Transcript:
ENSMUST00000160834.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
14
Exon/intron indexes:
25,26,27,28,29,30,31,32,33,34,35,36,37,38
Exon/intron offsets:
0,1220,2803,3980,6849,7452,10329,10953,12842,14703,19695,20483,21237,22269
Genomic length:
22371
Exon/intron sizes in nt:
16,08,95,61,17,61,54,10,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr1:60294211-60296588|chr1:60318959-60320405
Splice length:
2520
Other database IDs:
CIRCpedia: MMU_CIRCpedia_37776
circAtlas: hsa-NBEAL1_0260, CIRCpedia: HSA_CIRCpedia_163993
Human Ortholog IDs:
Mature spliced Sequence:
GTCATGGAACAGATGTTAAAATGCACAAATGTTTATGAACGTAGTAAACAACGTATCCGTCTTAGAGAAGTTGGCTATTCAGGAATTGGACTCCTCCTTAATGAAGCACCAGTTAACACATCTCTTATTAAAAACCTTGTTAACCAAATCATTAATACAGATCCTGTTATTAATTTCAAAGATCTATTATCTGTAGTATATATCTGTCACAGAACACATGTAAATGTCAGAGTGGTCCTCTGTAGAAAGATATTACAGATTTTGCAGTCCCAGCCAGATGCAGCATACCAGATATCACAACAAGTGGGTTGGCAAGACACCTTTGTTAGACTTTTCTTAAAAGCAAATTTTGAAAATGTAAATTCCCTTAACAAACACGGGAAGACAGTTTCAATGAAAGAAAATAAAAATATGTCAACTGAAGATGTTAAGAGGAACTTTGAAAAACTGGATGATGAAAAAATGATGTTAGCTGACGTATCTTCAGACCATTGGAGTTTAGAAGATAGTCAGTCCCTGAACTCAAACACACCATTATTTCAAGAAGATAGCTCTGAAGGAGAATTATCTTTCAAGTCAGAGAATCAAGAAGAATTTTGGCATAGTAATACTTCCCATCTGAGCTTAGATCTCAGTGGAATTGATGCATGTGAACTGAGCGACAGTGGAAGTCAAGTGCCAGACAGTTCACCTAGCACTCCATCTCCAGTAGAGTCTACTAAGTCATTTTCTTTGCAGTCTGACAAAGAAAGAAGCATCACGAATGAAATGGGCTTCAGTGATGACTTCTCTTTTCTTGAAAGCCAAGAGAGATGTGAAGAAGAACTTTTTCAGCTGCTTACAAATATTTTGAATTATGTTATGTGTAAAGGACTAGAAAAGTCTGATGAAGACACTTGGATTGAAAGAGGACAGGTGTTTTCAGCACTAACTAAGCCAGGAATATCAAGTGAGCTGCTTCGACCCTCAGATGAAGTAAAACTAACTTTGCTACAGAAGATGTTAGAGTGGGCAATCACAGAAAATAGAGATGCAAAAAGTAGTCCTGTAACTGCTGAAAATGCCTTCCGACTCATGTTGATCGTACAAGACTTTCTGCAATCAGAAGGACTGGTTAATTCAAACATGTGGACTGAGAAGCTTTTAGAAGATGCCACGCAGCTCCTAGACTGTCTGTCAGTATGGTATTCTGAGAATCCAGCATGGGTAAAACTCTCTCAGATTCAGACCCAGCTGCTTCTAGAGTTCATCGGAAGAGGCAGTTTGCAGGTTTGTGCAATGGCATCAGCTAAGCTCAATGCTCTTCTTCAGACCAAAGTGATTGAAAATCAAGATGAAGCATGCTATATTTTAGGGAAACTAGAACATATTCTAAGTCGGTCAATCAAGGAACAGACAGAAATCTATTCATTTCTGATTCCCCTTCTCCGGACTCTGGTTTCTAAAATTTATGAGCTGCTTTTCATGAACTTGCACCTGCCTTCCTTGCCTTTTACTAATGGAAGCGCTTCATTTTTTGAAGATTTTCAAGAATACTGCAATTCCGAAGAGTGGCAAGTTTACATTGAAAAATACATTGTACCTTACATGAAGCAGTATGAAGCGCATACATTTTATGATGGCCATGAGAATATGGCACTTTACTGGAAGAATTGTTATGAAGCCTTAATGGTGAATATGCATAAAAGAGACCGAGAAGGAGGAGAAAGCAAGCTGAAATTTCAGGAGTATTTTGTGGAGCCATTTAATCGAAAAGCCCGTCAAGAGAACTTGAGGTATAATAATATGATTAAACAACTTAACAGTCAGCAGTTGGCGGCTGTTAGACGCTGGAAGGCGATACGGCTCTATCTTACGTGCGAAAGGGGGCCTTGGGCTGAAAAGAAACAGAATCGAATTCACTGGAAACTAGCTAATGTTGAAAATTATTCCCGCATGAGACTTAAACTGGTACCAAATTATAATTTCAAAACCCACGAGGAAGCTAGTGCCTTAAGAGATAATTTAGGTGTTCAGCACTTACAGCCTTCCAGTGATTCATTGCTTTTGGAAGTAGTGAAACAAGTTAAACTCAGTGACATGGAGGAAGATAAACTAGAACTTCTTGAAGAGGACATGGCAGCTAGAGTAAATATTGATGAAAAGGAAGAACAGAATCAAAAGGAAAAATTGGTATTGACTGAAGACTGTGAACTCATTACTGTAATTGACATAATTCCCGGCAGATTAGAAGTCACTACTCAACACATTTACTTCCATGATAACAGTGTTGAAAAAGAAGATGGACTGGGCTTTGATTTCAAGTGGCCCCATTCTCAAATTCGAGAGATTCACTTACGTCGTTACAACTTAAGGAGATCAGCACTTGAGATTTTTCATGTTGATCAGTCCAACTACTTTCTCAATTTCAAAAAAGAGGTTAGAAACAAAATATATAGCAGACTTTTGTCACTTCATTCTCCTACTGGTTATGGAACAAGATCACCACAGGAGTTATTCAAAGCATCAGGGTTAACACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGAACAAGATCACCACAGGAG
59.96
AGGAGGAGTCCAATTCCTGA
57.35
147
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGAACAAGATCACCACAGGAGT
59.96
AGGAGGAGTCCAATTCCTGA
57.35
146
PCR template sequence:
TAGAAACAAAATATATAGCAGACTTTTGTCACTTCATTCTCCTACTGGTTATGGAACAAGATCACCACAGGAGTTATTCAAAGCATCAGGGTTAACACAGGTCATGGAACAGATGTTAAAATGCACAAATGTTTATGAACGTAGTAAACAACGTATCCGTCTTAGAGAAGTTGGCTATTCAGGAATTGGACTCCTCCTTA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
13
Interacting miRNAs (#binding sites)
mmu-miR-1198-5p(3), mmu-miR-335-3p(2), mmu-miR-20b-5p(1), mmu-miR-16-5p(1), mmu-miR-32-5p(1), mmu-miR-101b-3p(1), mmu-miR-25-3p(1), mmu-miR-431-5p(1), mmu-miR-22-3p(1), mmu-miR-212-3p(1), mmu-miR-382-5p(1), mmu-miR-467b-5p(1), mmu-miR-680(1)
siRNAs for circular RNA silencing
Junction sequence
AGGGTTAACACAGGTCATGGAACAGA
siRNA sets
CCATGACCTGTGTTAACCC, TTCCATGACCTGTGTTAAC, TCCATGACCTGTGTTAACC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCNBEAL1_2520_ORF_1
0.8818
0.004396416
1
5037
ORF sequence:
ATGGAACAGATGTTAAAATGCACAAATGTTTATGAACGTAGTAAACAACGTATCCGTCTTAGAGAAGTTGGCTATTCAGGAATTGGACTCCTCCTTAATGAAGCACCAGTTAACACATCTCTTATTAAAAACCTTGTTAACCAAATCATTAATACAGATCCTGTTATTAATTTCAAAGATCTATTATCTGTAGTATATATCTGTCACAGAACACATGTAAATGTCAGAGTGGTCCTCTGTAGAAAGATATTACAGATTTTGCAGTCCCAGCCAGATGCAGCATACCAGATATCACAACAAGTGGGTTGGCAAGACACCTTTGTTAGACTTTTCTTAAAAGCAAATTTTGAAAATGTAAATTCCCTTAACAAACACGGGAAGACAGTTTCAATGAAAGAAAATAAAAATATGTCAACTGAAGATGTTAAGAGGAACTTTGAAAAACTGGATGATGAAAAAATGATGTTAGCTGACGTATCTTCAGACCATTGGAGTTTAGAAGATAGTCAGTCCCTGAACTCAAACACACCATTATTTCAAGAAGATAGCTCTGAAGGAGAATTATCTTTCAAGTCAGAGAATCAAGAAGAATTTTGGCATAGTAATACTTCCCATCTGAGCTTAGATCTCAGTGGAATTGATGCATGTGAACTGAGCGACAGTGGAAGTCAAGTGCCAGACAGTTCACCTAGCACTCCATCTCCAGTAGAGTCTACTAAGTCATTTTCTTTGCAGTCTGACAAAGAAAGAAGCATCACGAATGAAATGGGCTTCAGTGATGACTTCTCTTTTCTTGAAAGCCAAGAGAGATGTGAAGAAGAACTTTTTCAGCTGCTTACAAATATTTTGAATTATGTTATGTGTAAAGGACTAGAAAAGTCTGATGAAGACACTTGGATTGAAAGAGGACAGGTGTTTTCAGCACTAACTAAGCCAGGAATATCAAGTGAGCTGCTTCGACCCTCAGATGAAGTAAAACTAACTTTGCTACAGAAGATGTTAGAGTGGGCAATCACAGAAAATAGAGATGCAAAAAGTAGTCCTGTAACTGCTGAAAATGCCTTCCGACTCATGTTGATCGTACAAGACTTTCTGCAATCAGAAGGACTGGTTAATTCAAACATGTGGACTGAGAAGCTTTTAGAAGATGCCACGCAGCTCCTAGACTGTCTGTCAGTATGGTATTCTGAGAATCCAGCATGGGTAAAACTCTCTCAGATTCAGACCCAGCTGCTTCTAGAGTTCATCGGAAGAGGCAGTTTGCAGGTTTGTGCAATGGCATCAGCTAAGCTCAATGCTCTTCTTCAGACCAAAGTGATTGAAAATCAAGATGAAGCATGCTATATTTTAGGGAAACTAGAACATATTCTAAGTCGGTCAATCAAGGAACAGACAGAAATCTATTCATTTCTGATTCCCCTTCTCCGGACTCTGGTTTCTAAAATTTATGAGCTGCTTTTCATGAACTTGCACCTGCCTTCCTTGCCTTTTACTAATGGAAGCGCTTCATTTTTTGAAGATTTTCAAGAATACTGCAATTCCGAAGAGTGGCAAGTTTACATTGAAAAATACATTGTACCTTACATGAAGCAGTATGAAGCGCATACATTTTATGATGGCCATGAGAATATGGCACTTTACTGGAAGAATTGTTATGAAGCCTTAATGGTGAATATGCATAAAAGAGACCGAGAAGGAGGAGAAAGCAAGCTGAAATTTCAGGAGTATTTTGTGGAGCCATTTAATCGAAAAGCCCGTCAAGAGAACTTGAGGTATAATAATATGATTAAACAACTTAACAGTCAGCAGTTGGCGGCTGTTAGACGCTGGAAGGCGATACGGCTCTATCTTACGTGCGAAAGGGGGCCTTGGGCTGAAAAGAAACAGAATCGAATTCACTGGAAACTAGCTAATGTTGAAAATTATTCCCGCATGAGACTTAAACTGGTACCAAATTATAATTTCAAAACCCACGAGGAAGCTAGTGCCTTAAGAGATAATTTAGGTGTTCAGCACTTACAGCCTTCCAGTGATTCATTGCTTTTGGAAGTAGTGAAACAAGTTAAACTCAGTGACATGGAGGAAGATAAACTAGAACTTCTTGAAGAGGACATGGCAGCTAGAGTAAATATTGATGAAAAGGAAGAACAGAATCAAAAGGAAAAATTGGTATTGACTGAAGACTGTGAACTCATTACTGTAATTGACATAATTCCCGGCAGATTAGAAGTCACTACTCAACACATTTACTTCCATGATAACAGTGTTGAAAAAGAAGATGGACTGGGCTTTGATTTCAAGTGGCCCCATTCTCAAATTCGAGAGATTCACTTACGTCGTTACAACTTAAGGAGATCAGCACTTGAGATTTTTCATGTTGATCAGTCCAACTACTTTCTCAATTTCAAAAAAGAGGTTAGAAACAAAATATATAGCAGACTTTTGTCACTTCATTCTCCTACTGGTTATGGAACAAGATCACCACAGGAGTTATTCAAAGCATCAGGGTTAACACAGGTCATGGAACAGATGTTAAAATGCACAAATGTTTATGAACGTAGTAAACAACGTATCCGTCTTAGAGAAGTTGGCTATTCAGGAATTGGACTCCTCCTTAATGAAGCACCAGTTAACACATCTCTTATTAAAAACCTTGTTAACCAAATCATTAATACAGATCCTGTTATTAATTTCAAAGATCTATTATCTGTAGTATATATCTGTCACAGAACACATGTAAATGTCAGAGTGGTCCTCTGTAGAAAGATATTACAGATTTTGCAGTCCCAGCCAGATGCAGCATACCAGATATCACAACAAGTGGGTTGGCAAGACACCTTTGTTAGACTTTTCTTAAAAGCAAATTTTGAAAATGTAAATTCCCTTAACAAACACGGGAAGACAGTTTCAATGAAAGAAAATAAAAATATGTCAACTGAAGATGTTAAGAGGAACTTTGAAAAACTGGATGATGAAAAAATGATGTTAGCTGACGTATCTTCAGACCATTGGAGTTTAGAAGATAGTCAGTCCCTGAACTCAAACACACCATTATTTCAAGAAGATAGCTCTGAAGGAGAATTATCTTTCAAGTCAGAGAATCAAGAAGAATTTTGGCATAGTAATACTTCCCATCTGAGCTTAGATCTCAGTGGAATTGATGCATGTGAACTGAGCGACAGTGGAAGTCAAGTGCCAGACAGTTCACCTAGCACTCCATCTCCAGTAGAGTCTACTAAGTCATTTTCTTTGCAGTCTGACAAAGAAAGAAGCATCACGAATGAAATGGGCTTCAGTGATGACTTCTCTTTTCTTGAAAGCCAAGAGAGATGTGAAGAAGAACTTTTTCAGCTGCTTACAAATATTTTGAATTATGTTATGTGTAAAGGACTAGAAAAGTCTGATGAAGACACTTGGATTGAAAGAGGACAGGTGTTTTCAGCACTAACTAAGCCAGGAATATCAAGTGAGCTGCTTCGACCCTCAGATGAAGTAAAACTAACTTTGCTACAGAAGATGTTAGAGTGGGCAATCACAGAAAATAGAGATGCAAAAAGTAGTCCTGTAACTGCTGAAAATGCCTTCCGACTCATGTTGATCGTACAAGACTTTCTGCAATCAGAAGGACTGGTTAATTCAAACATGTGGACTGAGAAGCTTTTAGAAGATGCCACGCAGCTCCTAGACTGTCTGTCAGTATGGTATTCTGAGAATCCAGCATGGGTAAAACTCTCTCAGATTCAGACCCAGCTGCTTCTAGAGTTCATCGGAAGAGGCAGTTTGCAGGTTTGTGCAATGGCATCAGCTAAGCTCAATGCTCTTCTTCAGACCAAAGTGATTGAAAATCAAGATGAAGCATGCTATATTTTAGGGAAACTAGAACATATTCTAAGTCGGTCAATCAAGGAACAGACAGAAATCTATTCATTTCTGATTCCCCTTCTCCGGACTCTGGTTTCTAAAATTTATGAGCTGCTTTTCATGAACTTGCACCTGCCTTCCTTGCCTTTTACTAATGGAAGCGCTTCATTTTTTGAAGATTTTCAAGAATACTGCAATTCCGAAGAGTGGCAAGTTTACATTGAAAAATACATTGTACCTTACATGAAGCAGTATGAAGCGCATACATTTTATGATGGCCATGAGAATATGGCACTTTACTGGAAGAATTGTTATGAAGCCTTAATGGTGAATATGCATAAAAGAGACCGAGAAGGAGGAGAAAGCAAGCTGAAATTTCAGGAGTATTTTGTGGAGCCATTTAATCGAAAAGCCCGTCAAGAGAACTTGAGGTATAATAATATGATTAAACAACTTAACAGTCAGCAGTTGGCGGCTGTTAGACGCTGGAAGGCGATACGGCTCTATCTTACGTGCGAAAGGGGGCCTTGGGCTGAAAAGAAACAGAATCGAATTCACTGGAAACTAGCTAATGTTGAAAATTATTCCCGCATGAGACTTAAACTGGTACCAAATTATAATTTCAAAACCCACGAGGAAGCTAGTGCCTTAAGAGATAATTTAGGTGTTCAGCACTTACAGCCTTCCAGTGATTCATTGCTTTTGGAAGTAGTGAAACAAGTTAAACTCAGTGACATGGAGGAAGATAAACTAGAACTTCTTGAAGAGGACATGGCAGCTAGAGTAAATATTGATGAAAAGGAAGAACAGAATCAAAAGGAAAAATTGGTATTGACTGAAGACTGTGAACTCATTACTGTAATTGACATAATTCCCGGCAGATTAGAAGTCACTACTCAACACATTTACTTCCATGATAACAGTGTTGAAAAAGAAGATGGACTGGGCTTTGATTTCAAGTGGCCCCATTCTCAAATTCGAGAGATTCACTTACGTCGTTACAACTTAAGGAGATCAGCACTTGAGATTTTTCATGTTGATCAGTCCAACTACTTTCTCAATTTCAAAAAAGAGGTTAGAAACAAAATATATAGCAGACTTTTGTCACTTCATTCTCCTACTGGTTATGGAACAAGATCACCACAGGAGTTATTCAAAGCATCAGGGTTAACACAG
Peptide sequence:
MEQMLKCTNVYERSKQRIRLREVGYSGIGLLLNEAPVNTSLIKNLVNQIINTDPVINFKDLLSVVYICHRTHVNVRVVLCRKILQILQSQPDAAYQISQQVGWQDTFVRLFLKANFENVNSLNKHGKTVSMKENKNMSTEDVKRNFEKLDDEKMMLADVSSDHWSLEDSQSLNSNTPLFQEDSSEGELSFKSENQEEFWHSNTSHLSLDLSGIDACELSDSGSQVPDSSPSTPSPVESTKSFSLQSDKERSITNEMGFSDDFSFLESQERCEEELFQLLTNILNYVMCKGLEKSDEDTWIERGQVFSALTKPGISSELLRPSDEVKLTLLQKMLEWAITENRDAKSSPVTAENAFRLMLIVQDFLQSEGLVNSNMWTEKLLEDATQLLDCLSVWYSENPAWVKLSQIQTQLLLEFIGRGSLQVCAMASAKLNALLQTKVIENQDEACYILGKLEHILSRSIKEQTEIYSFLIPLLRTLVSKIYELLFMNLHLPSLPFTNGSASFFEDFQEYCNSEEWQVYIEKYIVPYMKQYEAHTFYDGHENMALYWKNCYEALMVNMHKRDREGGESKLKFQEYFVEPFNRKARQENLRYNNMIKQLNSQQLAAVRRWKAIRLYLTCERGPWAEKKQNRIHWKLANVENYSRMRLKLVPNYNFKTHEEASALRDNLGVQHLQPSSDSLLLEVVKQVKLSDMEEDKLELLEEDMAARVNIDEKEEQNQKEKLVLTEDCELITVIDIIPGRLEVTTQHIYFHDNSVEKEDGLGFDFKWPHSQIREIHLRRYNLRRSALEIFHVDQSNYFLNFKKEVRNKIYSRLLSLHSPTGYGTRSPQELFKASGLTQVMEQMLKCTNVYERSKQRIRLREVGYSGIGLLLNEAPVNTSLIKNLVNQIINTDPVINFKDLLSVVYICHRTHVNVRVVLCRKILQILQSQPDAAYQISQQVGWQDTFVRLFLKANFENVNSLNKHGKTVSMKENKNMSTEDVKRNFEKLDDEKMMLADVSSDHWSLEDSQSLNSNTPLFQEDSSEGELSFKSENQEEFWHSNTSHLSLDLSGIDACELSDSGSQVPDSSPSTPSPVESTKSFSLQSDKERSITNEMGFSDDFSFLESQERCEEELFQLLTNILNYVMCKGLEKSDEDTWIERGQVFSALTKPGISSELLRPSDEVKLTLLQKMLEWAITENRDAKSSPVTAENAFRLMLIVQDFLQSEGLVNSNMWTEKLLEDATQLLDCLSVWYSENPAWVKLSQIQTQLLLEFIGRGSLQVCAMASAKLNALLQTKVIENQDEACYILGKLEHILSRSIKEQTEIYSFLIPLLRTLVSKIYELLFMNLHLPSLPFTNGSASFFEDFQEYCNSEEWQVYIEKYIVPYMKQYEAHTFYDGHENMALYWKNCYEALMVNMHKRDREGGESKLKFQEYFVEPFNRKARQENLRYNNMIKQLNSQQLAAVRRWKAIRLYLTCERGPWAEKKQNRIHWKLANVENYSRMRLKLVPNYNFKTHEEASALRDNLGVQHLQPSSDSLLLEVVKQVKLSDMEEDKLELLEEDMAARVNIDEKEEQNQKEKLVLTEDCELITVIDIIPGRLEVTTQHIYFHDNSVEKEDGLGFDFKWPHSQIREIHLRRYNLRRSALEIFHVDQSNYFLNFKKEVRNKIYSRLLSLHSPTGYGTRSPQELFKASGLTQ
IRESfinder result
IRESfinder score
0.748075975
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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