top of page
mmu_circNbeal1_1124
Basic information of circular RNA
PanCircBase ID:
mmu_circNbeal1_1124
mm39_Chr. Position ID:
chr1|60263783|60276375|+
Gene name:
Nbeal1
Transcript:
ENSMUST00000160834.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
10,11,12,13,14,15
Exon/intron offsets:
0,5977,10172,10356,11746,12472
Genomic length:
12592
Exon/intron sizes in nt:
1,07,84,99,58,11,33,120
Flanking exon/introns:
chr1:60261532-60263783|chr1:60276375-60277730
Splice length:
1124
Other database IDs:
CIRCpedia: MMU_CIRCpedia_24816, circAtlas: mmu-Nbeal1_0025
Human Ortholog IDs:
Mature spliced Sequence:
ATACCATCACATCCATGTTAGATTGCACAGACAGGCCCGTTCTTCAGGCCATTTTTCTCAACAGTAATTGCTTTGAACATCTCATCCGACTTCTACAGAACTGCAAGGTATTCCAGGGGCAGTTGGATTGCTTGGCTGTGTCAGCCATTCAGGCTTTGACAGCAGTGATGAAGAGTTCTCCAGCTGCTAAGGAAGTATTTAAAGAAAGAATTGGCTATATGCACATGTTTGAGGTATTAAAATCTTTGGGCCAGCCACCACAAGAATTGCTTAAAGAACTTATGAACATGGCTGTTGAGGGTGATCACACTTCAGTTGGCATTTTGGGCATTAGTAATGTCCAACCTCTCTTGGTTCTTATCCAGTGGCTTCCGGAGCTAGAATCTCATGACCTGCAAATCTTTGTCTCTGATTGGCTGAAAAAAATTTGTTGCATTAATAAGCAGAGCAGAACTACCTGTGTGAATGCAAACATGGGAATAAAAATCATTGAAGCCCTTGAATCCCATTCTTCCCTACATCGAACTTGTGCTGAGAACTTGATTGCAATCCATGGTTCATTGGGGAGTCAGTCTGTGAGCTCAGAAGAAATCCGTCGGCTTCTGCGACTGCTGAGAGTAGATGAATCAGAGTGTATTCACCCATATACCATTCCTGTGACTCGTGCAGTCCTGACAATGGCACGGAAACTCAGTTTTGAGAGTGCACTGCAGTATTTTAATTTGTCACATAGCATGGCCGGAATTTCTGTGCCTGCTATTCAGAGATGGCCTGGGTCTGCATTTTCCTTCACTGCTTGGTTTTGCTTAGATCAGGATCCGTTGACCCTTGGGAGTGCCAACAAAGGAGGAAAAAGAAAACAATTATATAGCTTTTTTACAGGAAGTGGAATGGGTTTTGAAGCTTTCATTACCCACTCAGGTGTGCTGGTTGTGGCAGTGTGCACAAAGAGAGAGTACGCGACAGTTATGCTTCCTGACCACAGCTTCTGTGATTCTCTCTGGCATAACATAACCATCGTTCACATGCCTGGAAAAAGGCCCTTTGGCCAGAGTCTTGTCTATATCTATGACAATGGACAACAAAAGGTTTCTGCCCCTCTCAGATTTCCTGCCATGAATGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCTGGAAAAAGGCCCTTTGG
59.31
CTGAAGAACGGGCCTGTCTG
60.67
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCTGGAAAAAGGCCCTTTGG
59.31
GAAGAACGGGCCTGTCTGTG
60.95
141
PCR template sequence:
CATGCCTGGAAAAAGGCCCTTTGGCCAGAGTCTTGTCTATATCTATGACAATGGACAACAAAAGGTTTCTGCCCCTCTCAGATTTCCTGCCATGAATGAGATACCATCACATCCATGTTAGATTGCACAGACAGGCCCGTTCTTCAGGCCATTTTTCTCAACAGTAATTGCTTTGAACATCTCATCCGACTTCTACAGAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
12
Interacting miRNAs (#binding sites)
mmu-miR-31-5p(1), mmu-miR-200c-3p(1), mmu-miR-152-3p(1), mmu-miR-148b-3p(1), mmu-miR-148a-3p(1), mmu-miR-196a-5p(1), mmu-miR-214-3p(1), mmu-miR-127-3p(1), mmu-miR-335-3p(1), mmu-miR-3105-3p(1), mmu-miR-344-3p(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TGCCATGAATGAGATACCATCACATC
siRNA sets
ATGTGATGGTATCTCATTC, TGTGATGGTATCTCATTCA, TGGTATCTCATTCATGGCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCNBEAL1_1124_ORF_1
0.7548
-0.018185839
0.985157594
1131
ORF sequence:
ATGTTAGATTGCACAGACAGGCCCGTTCTTCAGGCCATTTTTCTCAACAGTAATTGCTTTGAACATCTCATCCGACTTCTACAGAACTGCAAGGTATTCCAGGGGCAGTTGGATTGCTTGGCTGTGTCAGCCATTCAGGCTTTGACAGCAGTGATGAAGAGTTCTCCAGCTGCTAAGGAAGTATTTAAAGAAAGAATTGGCTATATGCACATGTTTGAGGTATTAAAATCTTTGGGCCAGCCACCACAAGAATTGCTTAAAGAACTTATGAACATGGCTGTTGAGGGTGATCACACTTCAGTTGGCATTTTGGGCATTAGTAATGTCCAACCTCTCTTGGTTCTTATCCAGTGGCTTCCGGAGCTAGAATCTCATGACCTGCAAATCTTTGTCTCTGATTGGCTGAAAAAAATTTGTTGCATTAATAAGCAGAGCAGAACTACCTGTGTGAATGCAAACATGGGAATAAAAATCATTGAAGCCCTTGAATCCCATTCTTCCCTACATCGAACTTGTGCTGAGAACTTGATTGCAATCCATGGTTCATTGGGGAGTCAGTCTGTGAGCTCAGAAGAAATCCGTCGGCTTCTGCGACTGCTGAGAGTAGATGAATCAGAGTGTATTCACCCATATACCATTCCTGTGACTCGTGCAGTCCTGACAATGGCACGGAAACTCAGTTTTGAGAGTGCACTGCAGTATTTTAATTTGTCACATAGCATGGCCGGAATTTCTGTGCCTGCTATTCAGAGATGGCCTGGGTCTGCATTTTCCTTCACTGCTTGGTTTTGCTTAGATCAGGATCCGTTGACCCTTGGGAGTGCCAACAAAGGAGGAAAAAGAAAACAATTATATAGCTTTTTTACAGGAAGTGGAATGGGTTTTGAAGCTTTCATTACCCACTCAGGTGTGCTGGTTGTGGCAGTGTGCACAAAGAGAGAGTACGCGACAGTTATGCTTCCTGACCACAGCTTCTGTGATTCTCTCTGGCATAACATAACCATCGTTCACATGCCTGGAAAAAGGCCCTTTGGCCAGAGTCTTGTCTATATCTATGACAATGGACAACAAAAGGTTTCTGCCCCTCTCAGATTTCCTGCCATGAATGAGATACCATCACATCCATGTTAG
Peptide sequence:
MLDCTDRPVLQAIFLNSNCFEHLIRLLQNCKVFQGQLDCLAVSAIQALTAVMKSSPAAKEVFKERIGYMHMFEVLKSLGQPPQELLKELMNMAVEGDHTSVGILGISNVQPLLVLIQWLPELESHDLQIFVSDWLKKICCINKQSRTTCVNANMGIKIIEALESHSSLHRTCAENLIAIHGSLGSQSVSSEEIRRLLRLLRVDESECIHPYTIPVTRAVLTMARKLSFESALQYFNLSHSMAGISVPAIQRWPGSAFSFTAWFCLDQDPLTLGSANKGGKRKQLYSFFTGSGMGFEAFITHSGVLVVAVCTKREYATVMLPDHSFCDSLWHNITIVHMPGKRPFGQSLVYIYDNGQQKVSAPLRFPAMNEIPSHPC
IRESfinder result
IRESfinder score
0.751276037
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page