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mmu_circNaa25_910
Basic information of circular RNA
PanCircBase ID:
mmu_circNaa25_910
mm39_Chr. Position ID:
chr5|121564789|121575768|+
Gene name:
Naa25
Transcript:
ENSMUST00000042163.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
15,16,17,18,19,20,21
Exon/intron offsets:
0,3878,4733,8099,8733,9932,10881
Genomic length:
10979
Exon/intron sizes in nt:
100,152,126,244,124,66,98
Flanking exon/introns:
chr5:121564214-121564789|chr5:121575768-121576790
Splice length:
910
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CTATCTTCTGACCCGATATGCTGCATCCCTGGGTCAGTATGCTGCTGCCTCCCAGTCCTGTAACTTTGCACTCAGGTTTTTCCACTCCAACCAGAAAGATACCTCAGAATACATTATTCAAGCTTACAAATATGGTGCATTTGAGAAGATCCCAGAGTTTATCGCTTTTAGGAACAGGCTGAATAATTCTCTCCATTTTGCACAAGTACGCACTGAGCGGATGCTGTTAGACCTTCTACTTGAAGCAAACATATCAATTAGCTTGGCAGAAAGTATCAAATCAATGAATCTGAGGCCAGAGGAAGATGATGTTCCATGGGAAGATTTGCGAGACAACAGAGACTTGGATGTTTTCTTCAGTTGGGATCCAAAAGACAGGAATGTTTCTGAAGAGCATAAGAAACTCTCCTTGGAGGAAGAAACCATGTGGTTAAGAATTCGCTCCTTAACGTTGAGGCTGATCAGTGGACTTCCAAGTCTTACCCACCCTGTGGAGCCCAAGAACTCAGAGAAGATGTCAGAGAACGGTGTGTCCTCCCGGATCGACATCCTTCGTTTGCTCCTCCAACAGCTAGAGGTGGCCGTGGAGACGGGGAAGCGGTTTATTGAGAAGGAAATTCAGTACCCCTTCCTTGGGCCTGTTCCCACCAGGATGGGCAGGTTCTTCAGTTCTGGCTGCTGTCAGTGCCAAGTCCAGTCCTTCCATCTGGTCAGCGACATGTATGAGCTTGACACCAGTGGCTTAGAGGGTACAGTGGATATACAGGAACGAATAGAAAATAGTCTTGCATCTTTACTAGAACTATTGAAAGGTGTCTTCAGCACATGTAAAGGGGACCTCTTAGAAGTTACAGATGGCAATGTGAAGACCCAGCCTGCAGTCCTAGAGAATCTGGTCTTCTTCGTTGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCAATGTGAAGACCCAGCCT
60.61
GGAGTGGAAAAACCTGAGTGC
59.39
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGCAATGTGAAGACCCAGC
60.9
GGAGTGGAAAAACCTGAGTGC
59.39
143
PCR template sequence:
AGGTGTCTTCAGCACATGTAAAGGGGACCTCTTAGAAGTTACAGATGGCAATGTGAAGACCCAGCCTGCAGTCCTAGAGAATCTGGTCTTCTTCGTTGAGCTATCTTCTGACCCGATATGCTGCATCCCTGGGTCAGTATGCTGCTGCCTCCCAGTCCTGTAACTTTGCACTCAGGTTTTTCCACTCCAACCAGAAAGAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
15
Interacting miRNAs (#binding sites)
mmu-miR-23b-3p(1), mmu-miR-103-3p(1), mmu-miR-130a-3p(1), mmu-miR-19a-3p(1), mmu-miR-709(1), mmu-miR-326-3p(1), mmu-miR-23a-3p(1), mmu-miR-185-5p(1), mmu-miR-96-5p(1), mmu-miR-130b-3p(1), mmu-miR-301b-3p(1), mmu-miR-183-5p(1), mmu-miR-107-3p(1), mmu-miR-499-5p(1), mmu-miR-190b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CTTCTTCGTTGAGCTATCTTCTGACC
siRNA sets
GAAGATAGCTCAACGAAGA, TCAGAAGATAGCTCAACGA, AAGATAGCTCAACGAAGAA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCNAA25_910_ORF_1
0.9261
0.162639215
0.926587729
753
ORF sequence:
ATGCTGTTAGACCTTCTACTTGAAGCAAACATATCAATTAGCTTGGCAGAAAGTATCAAATCAATGAATCTGAGGCCAGAGGAAGATGATGTTCCATGGGAAGATTTGCGAGACAACAGAGACTTGGATGTTTTCTTCAGTTGGGATCCAAAAGACAGGAATGTTTCTGAAGAGCATAAGAAACTCTCCTTGGAGGAAGAAACCATGTGGTTAAGAATTCGCTCCTTAACGTTGAGGCTGATCAGTGGACTTCCAAGTCTTACCCACCCTGTGGAGCCCAAGAACTCAGAGAAGATGTCAGAGAACGGTGTGTCCTCCCGGATCGACATCCTTCGTTTGCTCCTCCAACAGCTAGAGGTGGCCGTGGAGACGGGGAAGCGGTTTATTGAGAAGGAAATTCAGTACCCCTTCCTTGGGCCTGTTCCCACCAGGATGGGCAGGTTCTTCAGTTCTGGCTGCTGTCAGTGCCAAGTCCAGTCCTTCCATCTGGTCAGCGACATGTATGAGCTTGACACCAGTGGCTTAGAGGGTACAGTGGATATACAGGAACGAATAGAAAATAGTCTTGCATCTTTACTAGAACTATTGAAAGGTGTCTTCAGCACATGTAAAGGGGACCTCTTAGAAGTTACAGATGGCAATGTGAAGACCCAGCCTGCAGTCCTAGAGAATCTGGTCTTCTTCGTTGAGCTATCTTCTGACCCGATATGCTGCATCCCTGGGTCAGTATGCTGCTGCCTCCCAGTCCTGTAA
Peptide sequence:
MLLDLLLEANISISLAESIKSMNLRPEEDDVPWEDLRDNRDLDVFFSWDPKDRNVSEEHKKLSLEEETMWLRIRSLTLRLISGLPSLTHPVEPKNSEKMSENGVSSRIDILRLLLQQLEVAVETGKRFIEKEIQYPFLGPVPTRMGRFFSSGCCQCQVQSFHLVSDMYELDTSGLEGTVDIQERIENSLASLLELLKGVFSTCKGDLLEVTDGNVKTQPAVLENLVFFVELSSDPICCIPGSVCCCLPVL
IRESfinder result
IRESfinder score
0.663045316
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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