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mmu_circNaa25_672
Basic information of circular RNA
PanCircBase ID:
mmu_circNaa25_672
mm39_Chr. Position ID:
chr5|121552551|121559466|+
Gene name:
Naa25
Transcript:
ENSMUST00000042163.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
6,7,8,9,10,11
Exon/intron offsets:
0,313,2888,3438,5974,6802
Genomic length:
6915
Exon/intron sizes in nt:
1,08,79,11,29,01,70,110
Flanking exon/introns:
chr5:121551144-121552551|chr5:121559466-121561597
Splice length:
672
Other database IDs:
circBase: hsa_circ_0028333, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_6787
Human Ortholog IDs:
Mature spliced Sequence:
TCTATATCCGCACGGGATGAGAACCTCTCCAAAACCATGTTTCTGCCCCTGGCGGAGAGAATGGTAGAGAAGATGGTGAAGGAGGACAAGATAGAGGCTGAAGCTGAGGTTGAACTTTATTACATGATTCTGGAGCGCTTGGGAAAGTACCAGGAAGCCCTGGATGTCATTAGAGGAAAATTAGGAGAGAAGTTGACAAGTGAAATTCAGAGTCGGGAAAACAAATGCATGGCCATGTACAAGAAGCTGAGCAAGTGGCCGGAGTGCAACGCCCTTTCCCGACGCCTCCTTCTGAAAAACTCAGATGACTGGCAGTTCTATCTGACTTATTTCGATTCTGTCTTTCGACTGATTGAGGAAGCCTGGACGCCGCCTGCTGAGGGGGAACACTCCTTAGAAGGGGAAGTACACTGCTCTGCAGAAGATGCTGTGAAGTTTATAGAGGACAGGATAACAGAGGCATCACAAAGCTCTCGACATGTCCGAGGCCCACACCTAGCTAAGCTGGAGCTCATCAGGCGCTTGCGGAGCCAAGGCTGTAATGACGAATACAAGCTGGGTGACCCTGAAGAGTTAATGTTCCAGTATTTCAAGAAGTTCGGGGATAAGCCATGCTGCTTCACAGACCTGAAGGTGTTCGTTGACCTCTTGCCTGCCGCCCAGTGCACACAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTTCGGGGATAAGCCATGCT
60.18
CTACCATTCTCTCCGCCAGG
59.61
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGTTCGGGGATAAGCCATGC
60.18
CTACCATTCTCTCCGCCAGG
59.61
144
PCR template sequence:
GTTAATGTTCCAGTATTTCAAGAAGTTCGGGGATAAGCCATGCTGCTTCACAGACCTGAAGGTGTTCGTTGACCTCTTGCCTGCCGCCCAGTGCACACAATCTATATCCGCACGGGATGAGAACCTCTCCAAAACCATGTTTCTGCCCCTGGCGGAGAGAATGGTAGAGAAGATGGTGAAGGAGGACAAGATAGAGGCTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-339-5p(1), mmu-miR-214-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CCAGTGCACACAATCTATATCCGCAC
siRNA sets
ATATAGATTGTGTGCACTG, GATATAGATTGTGTGCACT, TATAGATTGTGTGCACTGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCNAA25_672_ORF_1
1.098
0.331666163
0.999480622
1308
ORF sequence:
ATGTTTCTGCCCCTGGCGGAGAGAATGGTAGAGAAGATGGTGAAGGAGGACAAGATAGAGGCTGAAGCTGAGGTTGAACTTTATTACATGATTCTGGAGCGCTTGGGAAAGTACCAGGAAGCCCTGGATGTCATTAGAGGAAAATTAGGAGAGAAGTTGACAAGTGAAATTCAGAGTCGGGAAAACAAATGCATGGCCATGTACAAGAAGCTGAGCAAGTGGCCGGAGTGCAACGCCCTTTCCCGACGCCTCCTTCTGAAAAACTCAGATGACTGGCAGTTCTATCTGACTTATTTCGATTCTGTCTTTCGACTGATTGAGGAAGCCTGGACGCCGCCTGCTGAGGGGGAACACTCCTTAGAAGGGGAAGTACACTGCTCTGCAGAAGATGCTGTGAAGTTTATAGAGGACAGGATAACAGAGGCATCACAAAGCTCTCGACATGTCCGAGGCCCACACCTAGCTAAGCTGGAGCTCATCAGGCGCTTGCGGAGCCAAGGCTGTAATGACGAATACAAGCTGGGTGACCCTGAAGAGTTAATGTTCCAGTATTTCAAGAAGTTCGGGGATAAGCCATGCTGCTTCACAGACCTGAAGGTGTTCGTTGACCTCTTGCCTGCCGCCCAGTGCACACAATCTATATCCGCACGGGATGAGAACCTCTCCAAAACCATGTTTCTGCCCCTGGCGGAGAGAATGGTAGAGAAGATGGTGAAGGAGGACAAGATAGAGGCTGAAGCTGAGGTTGAACTTTATTACATGATTCTGGAGCGCTTGGGAAAGTACCAGGAAGCCCTGGATGTCATTAGAGGAAAATTAGGAGAGAAGTTGACAAGTGAAATTCAGAGTCGGGAAAACAAATGCATGGCCATGTACAAGAAGCTGAGCAAGTGGCCGGAGTGCAACGCCCTTTCCCGACGCCTCCTTCTGAAAAACTCAGATGACTGGCAGTTCTATCTGACTTATTTCGATTCTGTCTTTCGACTGATTGAGGAAGCCTGGACGCCGCCTGCTGAGGGGGAACACTCCTTAGAAGGGGAAGTACACTGCTCTGCAGAAGATGCTGTGAAGTTTATAGAGGACAGGATAACAGAGGCATCACAAAGCTCTCGACATGTCCGAGGCCCACACCTAGCTAAGCTGGAGCTCATCAGGCGCTTGCGGAGCCAAGGCTGTAATGACGAATACAAGCTGGGTGACCCTGAAGAGTTAATGTTCCAGTATTTCAAGAAGTTCGGGGATAAGCCATGCTGCTTCACAGACCTGAAGGTGTTCGTTGACCTCTTGCCTGCCGCCCAGTGCACACAA
Peptide sequence:
MFLPLAERMVEKMVKEDKIEAEAEVELYYMILERLGKYQEALDVIRGKLGEKLTSEIQSRENKCMAMYKKLSKWPECNALSRRLLLKNSDDWQFYLTYFDSVFRLIEEAWTPPAEGEHSLEGEVHCSAEDAVKFIEDRITEASQSSRHVRGPHLAKLELIRRLRSQGCNDEYKLGDPEELMFQYFKKFGDKPCCFTDLKVFVDLLPAAQCTQSISARDENLSKTMFLPLAERMVEKMVKEDKIEAEAEVELYYMILERLGKYQEALDVIRGKLGEKLTSEIQSRENKCMAMYKKLSKWPECNALSRRLLLKNSDDWQFYLTYFDSVFRLIEEAWTPPAEGEHSLEGEVHCSAEDAVKFIEDRITEASQSSRHVRGPHLAKLELIRRLRSQGCNDEYKLGDPEELMFQYFKKFGDKPCCFTDLKVFVDLLPAAQCTQ
IRESfinder result
IRESfinder score
0.37458457
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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