top of page
mmu_circMyt1_829
Basic information of circular RNA
PanCircBase ID:
mmu_circMyt1_829
mm39_Chr. Position ID:
chr2|181448308|181457406|+
Gene name:
Myt1
Transcript:
ENSMUST00000081125.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
12,13,14,15,16,17,18
Exon/intron offsets:
0,875,3729,4649,4839,8533,9014
Genomic length:
9098
Exon/intron sizes in nt:
125,270,155,63,69,63,84
Flanking exon/introns:
chr2:181446934-181448308|chr2:181457406-181461799
Splice length:
829
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GCTTCGATTACTCTCATGATGCTGAGGCTGCACACATGGCTGCCACAGCCATCCTGAACCTCTCCACTCGATGTTGGGAGATGCCAGAGAACCTCAGCACAAAGCCACAGGACCTCCCCAGCAAGGCTGTGGACATTGAGGTAGATGAAAATGGAACTCTGGACTTGAGCATGCATAAACATCGCAAGCGGGAAAACACTTTCCCCAGCAGTAGCAGCTGCAGTAGCAGCCCTGGTGTCAAGTCTCCTGATGTCTCTCAACGTCAGAGCAGTACCAGTGCCCCCAGCAGCTCTATGACCTCACCCCAGTCCAGCCAGGCCTCTCGCCAGGATGAGTGGGACCGTCCTCTAGACTACACCAAACCTAGTCGGCTTCGAGAGGAAGAGCCTGAGGAGTCAGAGCCAGCAGCACACTCTTTTGCTTCTTCTGAAGCAGATGATCAGGAGGTGTCAGAAGAGAACTTTGAGGAGCGGAAGTATCCAGGGGAAGTCACCCTAACCAACTTTAAGCTGAAGTTTCTTTCCAAGGACATAAAGAAGGAACTTCTCACCTGTCCCACCCCCGGCTGTGACGGCAGTGGCCATATCACCGGGAACTATGCCTCCCATCGCAGCCTCTCTGGTTGCCCTCTTGCTGACAAGAGCCTCAGAAACCTCATGGCTGCCCACTCTGCTGACCTCAAGTGCCCCACACCTGGCTGTGATGGCTCTGGCCACATAACAGGGAACTATGCTTCACACCGGAGTTTGTCAGGCTGCCCACGTGCCAAGAAGAGTGGACTCAAGGTGGCGCCTACCAAGGATGACAAGGAGGACCCCGAGTTGATGAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGTGCCAAGAAGAGTGGACT
59.97
GTGCTGAGGTTCTCTGGCAT
60.04
168
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CGTGCCAAGAAGAGTGGACT
59.97
CCCAACATCGAGTGGAGAGG
59.82
146
PCR template sequence:
ATGCTTCACACCGGAGTTTGTCAGGCTGCCCACGTGCCAAGAAGAGTGGACTCAAGGTGGCGCCTACCAAGGATGACAAGGAGGACCCCGAGTTGATGAAGCTTCGATTACTCTCATGATGCTGAGGCTGCACACATGGCTGCCACAGCCATCCTGAACCTCTCCACTCGATGTTGGGAGATGCCAGAGAACCTCAGCAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-149-5p(1), mmu-miR-362-5p(1), mmu-miR-680(1), mmu-miR-484(1), mmu-miR-3061-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CCGAGTTGATGAAGCTTCGATTACTC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMYT1_829_ORF_1
0.9355
0.343105515
0.976512112
813
ORF sequence:
ATGGCTGCCACAGCCATCCTGAACCTCTCCACTCGATGTTGGGAGATGCCAGAGAACCTCAGCACAAAGCCACAGGACCTCCCCAGCAAGGCTGTGGACATTGAGGTAGATGAAAATGGAACTCTGGACTTGAGCATGCATAAACATCGCAAGCGGGAAAACACTTTCCCCAGCAGTAGCAGCTGCAGTAGCAGCCCTGGTGTCAAGTCTCCTGATGTCTCTCAACGTCAGAGCAGTACCAGTGCCCCCAGCAGCTCTATGACCTCACCCCAGTCCAGCCAGGCCTCTCGCCAGGATGAGTGGGACCGTCCTCTAGACTACACCAAACCTAGTCGGCTTCGAGAGGAAGAGCCTGAGGAGTCAGAGCCAGCAGCACACTCTTTTGCTTCTTCTGAAGCAGATGATCAGGAGGTGTCAGAAGAGAACTTTGAGGAGCGGAAGTATCCAGGGGAAGTCACCCTAACCAACTTTAAGCTGAAGTTTCTTTCCAAGGACATAAAGAAGGAACTTCTCACCTGTCCCACCCCCGGCTGTGACGGCAGTGGCCATATCACCGGGAACTATGCCTCCCATCGCAGCCTCTCTGGTTGCCCTCTTGCTGACAAGAGCCTCAGAAACCTCATGGCTGCCCACTCTGCTGACCTCAAGTGCCCCACACCTGGCTGTGATGGCTCTGGCCACATAACAGGGAACTATGCTTCACACCGGAGTTTGTCAGGCTGCCCACGTGCCAAGAAGAGTGGACTCAAGGTGGCGCCTACCAAGGATGACAAGGAGGACCCCGAGTTGATGAAGCTTCGATTACTCTCATGA
Peptide sequence:
MAATAILNLSTRCWEMPENLSTKPQDLPSKAVDIEVDENGTLDLSMHKHRKRENTFPSSSSCSSSPGVKSPDVSQRQSSTSAPSSSMTSPQSSQASRQDEWDRPLDYTKPSRLREEEPEESEPAAHSFASSEADDQEVSEENFEERKYPGEVTLTNFKLKFLSKDIKKELLTCPTPGCDGSGHITGNYASHRSLSGCPLADKSLRNLMAAHSADLKCPTPGCDGSGHITGNYASHRSLSGCPRAKKSGLKVAPTKDDKEDPELMKLRLLS
IRESfinder result
IRESfinder score
0.367050275
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page