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mmu_circMyrip_365
Basic information of circular RNA
PanCircBase ID:
mmu_circMyrip_365
mm39_Chr. Position ID:
chr9|120164663|120217399|+
Gene name:
Myrip
Transcript:
ENSMUST00000133173.3
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
2,3
Exon/intron offsets:
0,52514
Genomic length:
52736
Exon/intron sizes in nt:
1,43,222
Flanking exon/introns:
chr9:120130675-120164663|chr9:120217399-120246205
Splice length:
365
Other database IDs:
CIRCpedia: MMU_CIRCpedia_5059, circAtlas: mmu-Myrip_0001
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_45574
Human Ortholog IDs:
Mature spliced Sequence:
ATCTAGTTCAGTTGTCCGTGTTTAACTCTGATCATGGGGAGGAAGCTGGACCTGTCGGGTCTGACCGACGATGAGACCGAGCATGTTCTCCAAGTGGTGCAGAGAGACTTCAATCTTCGCAAAAAGGAGGAGGACCGGCTAAGTGAGATGAAGCAGAGGCTGGCCGAGGAGAACAGCAAGTGCAGCATCCTCTCGAAGCACCAGAAGTTCGTGGAGCGCTGCTGCATGCGGTGCTGCTCGCCCTTCACCTTCCTGGTCAACGCCAGGCGCCGCTGCGGGGAATGCAAGTTCAGCGTCTGCAAGAGCTGCTGCTCCTACCAGAAGCACGAGAAGCTATGGGTTTGCTGTGTCTGCCAGCAAGCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGGAATGCAAGTTCAGCGTC
59.83
GTCTCATCGTCGGTCAGACC
59.9
165
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGGAATGCAAGTTCAGCGTC
59.83
GGTCTCATCGTCGGTCAGAC
59.9
166
PCR template sequence:
GGCGCCGCTGCGGGGAATGCAAGTTCAGCGTCTGCAAGAGCTGCTGCTCCTACCAGAAGCACGAGAAGCTATGGGTTTGCTGTGTCTGCCAGCAAGCCAGATCTAGTTCAGTTGTCCGTGTTTAACTCTGATCATGGGGAGGAAGCTGGACCTGTCGGGTCTGACCGACGATGAGACCGAGCATGTTCTCCAAGTGGTGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-322-5p(2), mmu-miR-15b-5p(2), mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-383-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GCCAGCAAGCCAGATCTAGTTCAGTT
siRNA sets
AACTAGATCTGGCTTGCTG, AACTGAACTAGATCTGGCT, GAACTAGATCTGGCTTGCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMYRIP_365_ORF_1
1.0945
0.392832339
0.722629822
357
ORF sequence:
ATGGGGAGGAAGCTGGACCTGTCGGGTCTGACCGACGATGAGACCGAGCATGTTCTCCAAGTGGTGCAGAGAGACTTCAATCTTCGCAAAAAGGAGGAGGACCGGCTAAGTGAGATGAAGCAGAGGCTGGCCGAGGAGAACAGCAAGTGCAGCATCCTCTCGAAGCACCAGAAGTTCGTGGAGCGCTGCTGCATGCGGTGCTGCTCGCCCTTCACCTTCCTGGTCAACGCCAGGCGCCGCTGCGGGGAATGCAAGTTCAGCGTCTGCAAGAGCTGCTGCTCCTACCAGAAGCACGAGAAGCTATGGGTTTGCTGTGTCTGCCAGCAAGCCAGATCTAGTTCAGTTGTCCGTGTTTAA
Peptide sequence:
MGRKLDLSGLTDDETEHVLQVVQRDFNLRKKEEDRLSEMKQRLAEENSKCSILSKHQKFVERCCMRCCSPFTFLVNARRRCGECKFSVCKSCCSYQKHEKLWVCCVCQQARSSSVVRV
IRESfinder result
IRESfinder score
0.224426451
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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