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mmu_circMyo9a_946
Basic information of circular RNA
PanCircBase ID:
mmu_circMyo9a_946
mm39_Chr. Position ID:
chr9|59791762|59807744|+
Gene name:
Myo9a
Transcript:
ENSMUST00000128341.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
27,28,29,30,31,32,33
Exon/intron offsets:
0,4933,9661,10281,10713,12010,15870
Genomic length:
15982
Exon/intron sizes in nt:
1,13,14,32,24,11,71,89,00,000
Flanking exon/introns:
chr9:59781966-59791762|chr9:59807744-59810189
Splice length:
946
Other database IDs:
circAtlas: mmu-Myo9a_0016
circBase: hsa_circ_0000624, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_156514
Human Ortholog IDs:
Mature spliced Sequence:
ACATCACAACGATTTTCTTCAGTTGATGAGCAAGCAAAGCTCCATAAGGCTATGTCTCAAGGAGAGATTACAAAATTGGCAGTAAGACAGAAGGCTTCAGATTTGGACATAAGACCCCAGAGAGCGAAGATGAGATTCTGGGCCAAAGGGAAACAAGGAGAGAAGAAGACAACTAGAGTAAAACCTGCTTCCCAGTCGGAGATCTCATCATTCTTTCCTGGCCCAGATGTGACACCTGCTCATCCATTTTCTGACGAGTTAACTCAGTACCACCCAACACCTCCTTTGAGTCCAGAACTGCCTGGGAGTTGTCGAAAAGAGTTCAAAGAGAATAAGGAACCTTCTCCAAAGGCAAAGCGCAAGAGAGGTGTAAAAATCAGCAGTGTGGCCTTGGACTCTATGCATTGGCAAAATGACTCTGTCCAAATCATAGCAAGTGCCAGTGATTTAAAAAGTATGGATGAGTTTCTTCTGAAAAAGATGAATGACCTAGATAATGAAGACAGCAAGAAGGATACATTAGTGGATGTTGTATTTAAAAAAGCCCTGAAAGAATTTCGGCAGAATATCTTCAGCTCTTATTCATCTGCATTGGCGATGGATGATGGGAAAAGCATCCGCTATAAAGACCTATATGCGCTATTTGAACAGATTCTGGAAAAAACCATGAGATTGGAGCAACGTGATTGGAATGAATCTCCAGTGAGAGTTTGGGTTAACACTTTTAAGGTGTTTTTAGATGAATACATGAATGAATTCAAGACTTTGGATAGCACAGCCCCAAAGGTGCTTAAAACAGAAAGAAAGAAAAGGAGGAAAAAGGAAACCGATTTGGTGGAAGAGCACAATGGCCACATCTTTAAGGCCACCCAGTATAGCATCCCTACATACTGTGAATACTGTTCTTCCTTGATATGGATAATGGACAGAGCCTCTGTTTGCAAAT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCCACCCAGTATAGCATCCC
59.96
AGCCTTCTGTCTTACTGCCA
58.65
178
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGCCACCCAGTATAGCATCC
59.96
AGCCTTCTGTCTTACTGCCA
58.65
179
PCR template sequence:
AATGGCCACATCTTTAAGGCCACCCAGTATAGCATCCCTACATACTGTGAATACTGTTCTTCCTTGATATGGATAATGGACAGAGCCTCTGTTTGCAAATACATCACAACGATTTTCTTCAGTTGATGAGCAAGCAAAGCTCCATAAGGCTATGTCTCAAGGAGAGATTACAAAATTGGCAGTAAGACAGAAGGCTTCAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-326-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TCTGTTTGCAAATACATCACAACGAT
siRNA sets
ATCGTTGTGATGTATTTGC, TGATGTATTTGCAAACAGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMYO9A_946_ORF_1
0.7257
0.11412215
0.959883564
921
ORF sequence:
ATGTCTCAAGGAGAGATTACAAAATTGGCAGTAAGACAGAAGGCTTCAGATTTGGACATAAGACCCCAGAGAGCGAAGATGAGATTCTGGGCCAAAGGGAAACAAGGAGAGAAGAAGACAACTAGAGTAAAACCTGCTTCCCAGTCGGAGATCTCATCATTCTTTCCTGGCCCAGATGTGACACCTGCTCATCCATTTTCTGACGAGTTAACTCAGTACCACCCAACACCTCCTTTGAGTCCAGAACTGCCTGGGAGTTGTCGAAAAGAGTTCAAAGAGAATAAGGAACCTTCTCCAAAGGCAAAGCGCAAGAGAGGTGTAAAAATCAGCAGTGTGGCCTTGGACTCTATGCATTGGCAAAATGACTCTGTCCAAATCATAGCAAGTGCCAGTGATTTAAAAAGTATGGATGAGTTTCTTCTGAAAAAGATGAATGACCTAGATAATGAAGACAGCAAGAAGGATACATTAGTGGATGTTGTATTTAAAAAAGCCCTGAAAGAATTTCGGCAGAATATCTTCAGCTCTTATTCATCTGCATTGGCGATGGATGATGGGAAAAGCATCCGCTATAAAGACCTATATGCGCTATTTGAACAGATTCTGGAAAAAACCATGAGATTGGAGCAACGTGATTGGAATGAATCTCCAGTGAGAGTTTGGGTTAACACTTTTAAGGTGTTTTTAGATGAATACATGAATGAATTCAAGACTTTGGATAGCACAGCCCCAAAGGTGCTTAAAACAGAAAGAAAGAAAAGGAGGAAAAAGGAAACCGATTTGGTGGAAGAGCACAATGGCCACATCTTTAAGGCCACCCAGTATAGCATCCCTACATACTGTGAATACTGTTCTTCCTTGATATGGATAATGGACAGAGCCTCTGTTTGCAAATACATCACAACGATTTTCTTCAGTTGA
Peptide sequence:
MSQGEITKLAVRQKASDLDIRPQRAKMRFWAKGKQGEKKTTRVKPASQSEISSFFPGPDVTPAHPFSDELTQYHPTPPLSPELPGSCRKEFKENKEPSPKAKRKRGVKISSVALDSMHWQNDSVQIIASASDLKSMDEFLLKKMNDLDNEDSKKDTLVDVVFKKALKEFRQNIFSSYSSALAMDDGKSIRYKDLYALFEQILEKTMRLEQRDWNESPVRVWVNTFKVFLDEYMNEFKTLDSTAPKVLKTERKKRRKKETDLVEEHNGHIFKATQYSIPTYCEYCSSLIWIMDRASVCKYITTIFFS
IRESfinder result
IRESfinder score
0.589617998
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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