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mmu_circMyo6_866
Basic information of circular RNA
PanCircBase ID:
mmu_circMyo6_866
mm39_Chr. Position ID:
chr9|80165694|80177449|+
Gene name:
Myo6
Transcript:
ENSMUST00000035889.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
12,13,14,15,16,17,18
Exon/intron offsets:
0,2366,3861,5779,7713,11214,11581
Genomic length:
11755
Exon/intron sizes in nt:
1,45,15,89,27,31,28,96,000
Flanking exon/introns:
chr9:80162342-80165694|chr9:80177449-80181204
Splice length:
866
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGGCTGCAATCTGAAGAACAAATCTGCCCCATCCCTGGAATACTGTGCTGAGCTGCTGGGCTTGGACCAAGATGATCTTCGAGTCAGTTTAACCACTCGAGTCATGCTCACCACAGCAGGGGGCACCAAAGGAACCGTTATAAAGGTCCCCCTGAAAGTGGAGCAGGCAAACAATGCTCGCGATGCTCTGGCAAAGACTGTCTATAGCCATCTCTTTGATCACGTGGTGAACAGGGTAAATCAGTGCTTTCCTTTTGAAACATCTTCTTATTTTATTGGAGTCCTGGATATTGCTGGTTTCGAGTACTTTGAGCACAACAGCTTCGAGCAATTTTGTATCAACTATTGCAATGAGAAACTCCAACAGTTTTTTAATGAAAGGATCCTGAAGGAGGAACAAGAACTGTATCAGAAAGAGGGCCTGGGTGTTAATGAAGTCCATTACGTGGACAATCAGGACTGCATAGATTTAATCGAAGTGAAGTTAGTGGGGATCCTGGATATTCTGGATGAAGAAAATCGTCTTCCCCAGCCGAGTGACCAGCACTTCACGTCTGTGGTTCACCAGAAGCACAAAGACCACTTCCGGCTCACTATTCCCAGGAAATCTAAGCTGGCAGTCCACAGGAACCTCAGAGACGATGAAGGCTTTATCATCAGGCACTTTGCAGGGGCCGTGTGCTACGAGACAACCCAGTTTGTGGAGAAAAATAATGATGCACTACATATGTCTCTTGAATCCTTGATATGTGAGTCCAGGGATAAGTTTATAAGGGCATTATTTGAGTCATCTACAAATAACAACAAAGATACGAAACAGAAGGCTGGAAAACTTAGCTTCATCAGCGTGGGAAACAAGTTCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAAAGATACGAAACAGAAGGCTGG
60.14
AGATCATCTTGGTCCAAGCCC
59.79
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CAACAAAGATACGAAACAGAAGGCT
60.05
TCATCTTGGTCCAAGCCCAG
59.67
140
PCR template sequence:
GTTTATAAGGGCATTATTTGAGTCATCTACAAATAACAACAAAGATACGAAACAGAAGGCTGGAAAACTTAGCTTCATCAGCGTGGGAAACAAGTTCAAGGTGGCTGCAATCTGAAGAACAAATCTGCCCCATCCCTGGAATACTGTGCTGAGCTGCTGGGCTTGGACCAAGATGATCTTCGAGTCAGTTTAACCACTCG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
12
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-20b-5p(1), mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-miR-17-5p(1), mmu-miR-7b-5p(1), mmu-miR-106b-5p(1), mmu-miR-7a-5p(1), mmu-miR-218-5p(1), mmu-miR-370-3p(1), mmu-miR-383-5p(1), mmu-miR-5101(1)
siRNAs for circular RNA silencing
Junction sequence
AAACAAGTTCAAGGTGGCTGCAATCT
siRNA sets
ATTGCAGCCACCTTGAACT,GATTGCAGCCACCTTGAAC,TTGCAGCCACCTTGAACTT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMYO6_866_ORF_1
1.1615
0.227456263
0.968122309
777
ORF sequence:
ATGCTCACCACAGCAGGGGGCACCAAAGGAACCGTTATAAAGGTCCCCCTGAAAGTGGAGCAGGCAAACAATGCTCGCGATGCTCTGGCAAAGACTGTCTATAGCCATCTCTTTGATCACGTGGTGAACAGGGTAAATCAGTGCTTTCCTTTTGAAACATCTTCTTATTTTATTGGAGTCCTGGATATTGCTGGTTTCGAGTACTTTGAGCACAACAGCTTCGAGCAATTTTGTATCAACTATTGCAATGAGAAACTCCAACAGTTTTTTAATGAAAGGATCCTGAAGGAGGAACAAGAACTGTATCAGAAAGAGGGCCTGGGTGTTAATGAAGTCCATTACGTGGACAATCAGGACTGCATAGATTTAATCGAAGTGAAGTTAGTGGGGATCCTGGATATTCTGGATGAAGAAAATCGTCTTCCCCAGCCGAGTGACCAGCACTTCACGTCTGTGGTTCACCAGAAGCACAAAGACCACTTCCGGCTCACTATTCCCAGGAAATCTAAGCTGGCAGTCCACAGGAACCTCAGAGACGATGAAGGCTTTATCATCAGGCACTTTGCAGGGGCCGTGTGCTACGAGACAACCCAGTTTGTGGAGAAAAATAATGATGCACTACATATGTCTCTTGAATCCTTGATATGTGAGTCCAGGGATAAGTTTATAAGGGCATTATTTGAGTCATCTACAAATAACAACAAAGATACGAAACAGAAGGCTGGAAAACTTAGCTTCATCAGCGTGGGAAACAAGTTCAAGGTGGCTGCAATCTGA
Peptide sequence:
MLTTAGGTKGTVIKVPLKVEQANNARDALAKTVYSHLFDHVVNRVNQCFPFETSSYFIGVLDIAGFEYFEHNSFEQFCINYCNEKLQQFFNERILKEEQELYQKEGLGVNEVHYVDNQDCIDLIEVKLVGILDILDEENRLPQPSDQHFTSVVHQKHKDHFRLTIPRKSKLAVHRNLRDDEGFIIRHFAGAVCYETTQFVEKNNDALHMSLESLICESRDKFIRALFESSTNNNKDTKQKAGKLSFISVGNKFKVAAI
IRESfinder result
IRESfinder score
0.694090351
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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