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mmu_circMyo5b_297
Basic information of circular RNA
PanCircBase ID:
mmu_circMyo5b_297
mm39_Chr. Position ID:
chr18|74810491|74828554|+
Gene name:
Myo5b
Transcript:
ENSMUST00000074157.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
16,17,18
Exon/intron offsets:
0,5128,17951
Genomic length:
18063
Exon/intron sizes in nt:
98,87,112
Flanking exon/introns:
chr18:74809827-74810491|chr18:74828554-74831256
Splice length:
297
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TTTCGTACTTCCCTAAACCTGCTTATGGAGACCCTGAATGCCACAACGCCACACTATGTCCGGTGCATCAAGCCCAACGATGAAAAGCTTCCCTTCCACTTTGACCCCAAGAGAGCTGTACAGCAACTCCGAGCCTGCGGTGTGCTGGAGACCATTCGGATCAGCGCGGCAGGCTACCCATCCAGGTGGACCTACCATGACTTCTTCAACCGGTATCGGGTGTTGATGAAGAAGAGAGAGCTCACCAACACCGACAAGAAGAATATCTGCAAGTCTGTCCTGGAGAGTCTCATCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAGAGAGCTCACCAACACCG
60.39
TCATCGTTGGGCTTGATGCA
60.32
147
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCTCACCAACACCGACAAGA
60.53
TCATCGTTGGGCTTGATGCA
60.32
141
PCR template sequence:
TGACTTCTTCAACCGGTATCGGGTGTTGATGAAGAAGAGAGAGCTCACCAACACCGACAAGAAGAATATCTGCAAGTCTGTCCTGGAGAGTCTCATCAAGTTTCGTACTTCCCTAAACCTGCTTATGGAGACCCTGAATGCCACAACGCCACACTATGTCCGGTGCATCAAGCCCAACGATGAAAAGCTTCCCTTCCACT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-501-3p(1), mmu-miR-335-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GAGTCTCATCAAGTTTCGTACTTCCC
siRNA sets
GAAGTACGAAACTTGATGA, ACGAAACTTGATGAGACTC, GTACGAAACTTGATGAGAC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMYO5B_297_ORF_1
1.17
0.481145578
0.952541568
570
ORF sequence:
ATGGAGACCCTGAATGCCACAACGCCACACTATGTCCGGTGCATCAAGCCCAACGATGAAAAGCTTCCCTTCCACTTTGACCCCAAGAGAGCTGTACAGCAACTCCGAGCCTGCGGTGTGCTGGAGACCATTCGGATCAGCGCGGCAGGCTACCCATCCAGGTGGACCTACCATGACTTCTTCAACCGGTATCGGGTGTTGATGAAGAAGAGAGAGCTCACCAACACCGACAAGAAGAATATCTGCAAGTCTGTCCTGGAGAGTCTCATCAAGTTTCGTACTTCCCTAAACCTGCTTATGGAGACCCTGAATGCCACAACGCCACACTATGTCCGGTGCATCAAGCCCAACGATGAAAAGCTTCCCTTCCACTTTGACCCCAAGAGAGCTGTACAGCAACTCCGAGCCTGCGGTGTGCTGGAGACCATTCGGATCAGCGCGGCAGGCTACCCATCCAGGTGGACCTACCATGACTTCTTCAACCGGTATCGGGTGTTGATGAAGAAGAGAGAGCTCACCAACACCGACAAGAAGAATATCTGCAAGTCTGTCCTGGAGAGTCTCATCAAG
Peptide sequence:
METLNATTPHYVRCIKPNDEKLPFHFDPKRAVQQLRACGVLETIRISAAGYPSRWTYHDFFNRYRVLMKKRELTNTDKKNICKSVLESLIKFRTSLNLLMETLNATTPHYVRCIKPNDEKLPFHFDPKRAVQQLRACGVLETIRISAAGYPSRWTYHDFFNRYRVLMKKRELTNTDKKNICKSVLESLIK
IRESfinder result
IRESfinder score
0.190180686
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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