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mmu_circMyo3a_545
Basic information of circular RNA
PanCircBase ID:
mmu_circMyo3a_545
mm39_Chr. Position ID:
chr2|22287397|22328484|+
Gene name:
Myo3a
Transcript:
ENSMUST00000044749.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
6,7,8,9,10
Exon/intron offsets:
0,9219,11886,14976,40987
Genomic length:
41087
Exon/intron sizes in nt:
77,14,66,61,56,100
Flanking exon/introns:
chr2:22272014-22287397|chr2:22328484-22337181
Splice length:
545
Other database IDs:
circAtlas: mmu-Myo3a_0010
Human Ortholog IDs:
Mature spliced Sequence:
GTGTGTCTGCACAGCTCAGCAGCACCCGGCACCGCCTGAACACCTCCGTAGGAACACCATTTTGGATGGCTCCTGAGGTGATTGCTTGTGAACAGCAACTAGATACCACTTACGATGCTAGATGTGACACATGGTCCCTGGGTATCACGGCTATTGAGCTTGGTGATGGAGATCCTCCACTAGCTGAGCTGCATCCCATGAGAGCACTCTTCAAAATACCAAGGAATCCACCCCCTAAACTCCGGCAGCCTGAGCTGTGGTCCGCAGAGTTCAATGACTTCATTAGCAAATGCTTGACCAAAGATTATGAAAAGCGTCCAACAGTGTCAGATCTTTTGAAGCATAAATTTATTACTCAGATCGAGGGCAAAGATGTGATACTACAAAAACAACTAATGGAATTCATTGATATCCATCAATGCCTGGGAAGCACAGAGAAGGCCAGGCATGAACGAATTCATACCAAGAAAGGTAATTTAAATAGATCTCTCATTTCCAGTCTGAAGGATGTAGACGATTTGGCAACCCTAGATGTTCTGGATGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTTCCAGTCTGAAGGATGTAGACG
60.08
AAGCAATCACCTCAGGAGCC
60.03
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCAGTCTGAAGGATGTAGACGA
58.98
TGCTGTTCACAAGCAATCACC
59.66
147
PCR template sequence:
GCATGAACGAATTCATACCAAGAAAGGTAATTTAAATAGATCTCTCATTTCCAGTCTGAAGGATGTAGACGATTTGGCAACCCTAGATGTTCTGGATGAGGTGTGTCTGCACAGCTCAGCAGCACCCGGCACCGCCTGAACACCTCCGTAGGAACACCATTTTGGATGGCTCCTGAGGTGATTGCTTGTGAACAGCAACT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-328-3p(1), mmu-miR-3470b(1)
siRNAs for circular RNA silencing
Junction sequence
TGTTCTGGATGAGGTGTGTCTGCACA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMYO3A_545_ORF_1
1.0072
0.175329146
0.743942124
519
ORF sequence:
ATGGCTCCTGAGGTGATTGCTTGTGAACAGCAACTAGATACCACTTACGATGCTAGATGTGACACATGGTCCCTGGGTATCACGGCTATTGAGCTTGGTGATGGAGATCCTCCACTAGCTGAGCTGCATCCCATGAGAGCACTCTTCAAAATACCAAGGAATCCACCCCCTAAACTCCGGCAGCCTGAGCTGTGGTCCGCAGAGTTCAATGACTTCATTAGCAAATGCTTGACCAAAGATTATGAAAAGCGTCCAACAGTGTCAGATCTTTTGAAGCATAAATTTATTACTCAGATCGAGGGCAAAGATGTGATACTACAAAAACAACTAATGGAATTCATTGATATCCATCAATGCCTGGGAAGCACAGAGAAGGCCAGGCATGAACGAATTCATACCAAGAAAGGTAATTTAAATAGATCTCTCATTTCCAGTCTGAAGGATGTAGACGATTTGGCAACCCTAGATGTTCTGGATGAGGTGTGTCTGCACAGCTCAGCAGCACCCGGCACCGCCTGA
Peptide sequence:
MAPEVIACEQQLDTTYDARCDTWSLGITAIELGDGDPPLAELHPMRALFKIPRNPPPKLRQPELWSAEFNDFISKCLTKDYEKRPTVSDLLKHKFITQIEGKDVILQKQLMEFIDIHQCLGSTEKARHERIHTKKGNLNRSLISSLKDVDDLATLDVLDEVCLHSSAAPGTA
IRESfinder result
IRESfinder score
0.520434733
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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