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mmu_circMyo3a_417_1
Basic information of circular RNA
PanCircBase ID:
mmu_circMyo3a_417_1
mm39_Chr. Position ID:
chr2|22250326|22287474|+
Gene name:
Myo3a
Transcript:
ENSMUST00000044749.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
3,4,5,6
Exon/intron offsets:
0,20541,21588,37071
Genomic length:
37148
Exon/intron sizes in nt:
135,105,100,77
Flanking exon/introns:
chr2:22246046-22250326|chr2:22287474-22296616
Splice length:
417
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GATATCGATGAAGAGATTGAAGCAGAGTATAACATCTTAAGAACACTTTCTGACCACCCAAATGTGGTCAGATTCTACGGGATTTACTTTAAAAAGGACAAGATAAATGGAGATAAGCTATGGCTGGTTCTGGAGCTGTGCAATGGAGGATCAGTGACTGATCTTGTGAAAGGATTTCTGAAGAGGGGAGAAAGAATGAGCGAGCCTGTAATTGCATATATTTTACACGAAGCACTAATGGGACTTCAGCATCTTCATAGCAACAAAACCATCCACCGTGATGTCAAAGGAAATAACATCCTGTTGACTACTGAAGGTGGAGTAAAACTAGTAGATTTTGGTGTGTCTGCACAGCTCAGCAGCACCCGGCACCGCCTGAACACCTCCGTAGGAACACCATTTTGGATGGCTCCTGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTGTCTGCACAGCTCAGCA
60.6
TCCCGTAGAATCTGACCACA
57.77
157
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTGTCTGCACAGCTCAGCA
60.6
TCCCGTAGAATCTGACCACAT
58.25
157
PCR template sequence:
TGGAGTAAAACTAGTAGATTTTGGTGTGTCTGCACAGCTCAGCAGCACCCGGCACCGCCTGAACACCTCCGTAGGAACACCATTTTGGATGGCTCCTGAGGATATCGATGAAGAGATTGAAGCAGAGTATAACATCTTAAGAACACTTTCTGACCACCCAAATGTGGTCAGATTCTACGGGATTTACTTTAAAAAGGACA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-32-5p(1), mmu-miR-92a-3p(1), mmu-miR-136-5p(1), mmu-miR-363-3p(1), mmu-miR-199b-3p(1), mmu-miR-199a-3p(1), mmu-miR-540-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GATGGCTCCTGAGGATATCGATGAAG
siRNA sets
CTTCATCGATATCCTCAGG, TTCATCGATATCCTCAGGA, TCATCGATATCCTCAGGAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMYO3A_417_1_ORF_1
0.9887
0.155530469
0.860943511
639
ORF sequence:
ATGAGCGAGCCTGTAATTGCATATATTTTACACGAAGCACTAATGGGACTTCAGCATCTTCATAGCAACAAAACCATCCACCGTGATGTCAAAGGAAATAACATCCTGTTGACTACTGAAGGTGGAGTAAAACTAGTAGATTTTGGTGTGTCTGCACAGCTCAGCAGCACCCGGCACCGCCTGAACACCTCCGTAGGAACACCATTTTGGATGGCTCCTGAGGATATCGATGAAGAGATTGAAGCAGAGTATAACATCTTAAGAACACTTTCTGACCACCCAAATGTGGTCAGATTCTACGGGATTTACTTTAAAAAGGACAAGATAAATGGAGATAAGCTATGGCTGGTTCTGGAGCTGTGCAATGGAGGATCAGTGACTGATCTTGTGAAAGGATTTCTGAAGAGGGGAGAAAGAATGAGCGAGCCTGTAATTGCATATATTTTACACGAAGCACTAATGGGACTTCAGCATCTTCATAGCAACAAAACCATCCACCGTGATGTCAAAGGAAATAACATCCTGTTGACTACTGAAGGTGGAGTAAAACTAGTAGATTTTGGTGTGTCTGCACAGCTCAGCAGCACCCGGCACCGCCTGAACACCTCCGTAGGAACACCATTTTGGATGGCTCCTGAG
Peptide sequence:
MSEPVIAYILHEALMGLQHLHSNKTIHRDVKGNNILLTTEGGVKLVDFGVSAQLSSTRHRLNTSVGTPFWMAPEDIDEEIEAEYNILRTLSDHPNVVRFYGIYFKKDKINGDKLWLVLELCNGGSVTDLVKGFLKRGERMSEPVIAYILHEALMGLQHLHSNKTIHRDVKGNNILLTTEGGVKLVDFGVSAQLSSTRHRLNTSVGTPFWMAPE
IRESfinder result
IRESfinder score
0.483447799
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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