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mmu_circMyo1f_366
Basic information of circular RNA
PanCircBase ID:
mmu_circMyo1f_366
mm39_Chr. Position ID:
chr17|33823230|33823693|+
Gene name:
Myo1f
Transcript:
ENSMUST00000087605.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
26,27
Exon/intron offsets:
0,293
Genomic length:
463
Exon/intron sizes in nt:
1,96,170
Flanking exon/introns:
chr17:33821583-33823230|chr17:33823693-33826272
Splice length:
366
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTTTGAATCGAAATGGGGTACCACCCTCTTCCCAGGTAAGGTCCCTACCCCTGGAGATCACATCTGGTAGGAGTTCCCAGAGGCCTCCACGGGGCCCTCCATCCTCAACCCTAGGAGCCAGCAGACGTCCTCGAGCACGGCCACCTTCAGAGCACAGTACAGAATTCCTTAATGTGCCTGACCAGGGTGTGGCAGGCATGCAAAGGAAGCGAAGTATAGGGCAGAGACCTGTGCCAGGAGTGGGCCGGCCTAAGCCCCAACCACGGACACATGGCCCGAGGTGCCGGGCACTGTACCAATACATAGGCCAGGATGTGGATGAGCTGAGCTTTAACGTGAACGAAGTCATTGAGATCCTCATAGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCGGGCACTGTACCAATACA
59.75
TGTGATCTCCAGGGGTAGGG
60.03
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCGGGCACTGTACCAATACA
59.75
GAGGCCTCTGGGAACTCCTA
60.03
170
PCR template sequence:
ACACATGGCCCGAGGTGCCGGGCACTGTACCAATACATAGGCCAGGATGTGGATGAGCTGAGCTTTAACGTGAACGAAGTCATTGAGATCCTCATAGAAGGTTTGAATCGAAATGGGGTACCACCCTCTTCCCAGGTAAGGTCCCTACCCCTGGAGATCACATCTGGTAGGAGTTCCCAGAGGCCTCCACGGGGCCCTCC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-326-3p(1), mmu-miR-149-5p(1), mmu-miR-1198-5p(1), mmu-miR-697(1), mmu-miR-296-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ATCCTCATAGAAGGTTTGAATCGAAA
siRNA sets
TTTCGATTCAAACCTTCTA, GATTCAAACCTTCTATGAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMYO1F_366_ORF_1
0.7946
0.216378214
0.722243894
534
ORF sequence:
ATGCAAAGGAAGCGAAGTATAGGGCAGAGACCTGTGCCAGGAGTGGGCCGGCCTAAGCCCCAACCACGGACACATGGCCCGAGGTGCCGGGCACTGTACCAATACATAGGCCAGGATGTGGATGAGCTGAGCTTTAACGTGAACGAAGTCATTGAGATCCTCATAGAAGGTTTGAATCGAAATGGGGTACCACCCTCTTCCCAGGTAAGGTCCCTACCCCTGGAGATCACATCTGGTAGGAGTTCCCAGAGGCCTCCACGGGGCCCTCCATCCTCAACCCTAGGAGCCAGCAGACGTCCTCGAGCACGGCCACCTTCAGAGCACAGTACAGAATTCCTTAATGTGCCTGACCAGGGTGTGGCAGGCATGCAAAGGAAGCGAAGTATAGGGCAGAGACCTGTGCCAGGAGTGGGCCGGCCTAAGCCCCAACCACGGACACATGGCCCGAGGTGCCGGGCACTGTACCAATACATAGGCCAGGATGTGGATGAGCTGAGCTTTAACGTGAACGAAGTCATTGAGATCCTCATAGAA
Peptide sequence:
MQRKRSIGQRPVPGVGRPKPQPRTHGPRCRALYQYIGQDVDELSFNVNEVIEILIEGLNRNGVPPSSQVRSLPLEITSGRSSQRPPRGPPSSTLGASRRPRARPPSEHSTEFLNVPDQGVAGMQRKRSIGQRPVPGVGRPKPQPRTHGPRCRALYQYIGQDVDELSFNVNEVIEILIE
IRESfinder result
IRESfinder score
0.456091352
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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