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mmu_circMyh10_561
Basic information of circular RNA
PanCircBase ID:
mmu_circMyh10_561
mm39_Chr. Position ID:
chr11|68590035|68625241|+
Gene name:
Myh10
Transcript:
ENSMUST00000102611.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
2,3,4
Exon/intron offsets:
0,22110,35178
Genomic length:
35206
Exon/intron sizes in nt:
376,157,28
Flanking exon/introns:
chr11:68583008-68590035|chr11:68625241-68636142
Splice length:
561
Other database IDs:
circAtlas: mmu-Myh10_0006
Human Ortholog IDs:
Mature spliced Sequence:
AGGCAATTACTTTTGGGTCCTTCTGTTTACAATGGCCCAGAGAACTGGACTGGAGGATCCCGAGAGGTATCTCTTTGTGGACAGGGCTGTCATCTACAACCCTGCCACTCAAGCTGACTGGACAGCTAAAAAGCTGGTGTGGATTCCATCGGAACGCCATGGTTTTGAGGCAGCTAGTATTAAAGAAGAGCGGGGCGATGAGGTTATGGTGGAGCTGGCAGAGAATGGGAAGAAAGCAATGGTCAACAAAGATGACATTCAGAAGATGAACCCACCAAAGTTCTCCAAGGTGGAGGATATGGCAGAGCTGACATGCTTGAACGAAGCCTCTGTCTTACATAATTTGAAGGACCGCTACTATTCAGGACTTATCTATACTTACTCTGGACTCTTCTGTGTGGTGATAAATCCTTACAAGAACCTTCCAATTTACTCTGAGAATATTATTGAAATGTATAGAGGGAAGAAACGCCATGAGATGCCACCACACATCTACGCCATATCAGAGTCTGCTTACAGATGCATGCTTCAAGATCGTGAGGACCAGTCAATTCTATGCAC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCACCACACATCTACGCCAT
60.11
ACAGCCCTGTCCACAAAGAG
59.89
170
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCATGAGATGCCACCACACA
60.32
ACAGCCCTGTCCACAAAGAG
59.89
180
PCR template sequence:
GGAAGAAACGCCATGAGATGCCACCACACATCTACGCCATATCAGAGTCTGCTTACAGATGCATGCTTCAAGATCGTGAGGACCAGTCAATTCTATGCACAGGCAATTACTTTTGGGTCCTTCTGTTTACAATGGCCCAGAGAACTGGACTGGAGGATCCCGAGAGGTATCTCTTTGTGGACAGGGCTGTCATCTACAAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-326-3p(1), mmu-miR-339-5p(1), mmu-miR-191-5p(1), mmu-miR-1187(1), mmu-miR-296-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CAATTCTATGCACAGGCAATTACTTT
siRNA sets
AAAGTAATTGCCTGTGCAT, ATTGCCTGTGCATAGAATT, GTAATTGCCTGTGCATAGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMYH10_561_ORF_1
1.0091
0.272664724
0.996243902
1089
ORF sequence:
ATGGCCCAGAGAACTGGACTGGAGGATCCCGAGAGGTATCTCTTTGTGGACAGGGCTGTCATCTACAACCCTGCCACTCAAGCTGACTGGACAGCTAAAAAGCTGGTGTGGATTCCATCGGAACGCCATGGTTTTGAGGCAGCTAGTATTAAAGAAGAGCGGGGCGATGAGGTTATGGTGGAGCTGGCAGAGAATGGGAAGAAAGCAATGGTCAACAAAGATGACATTCAGAAGATGAACCCACCAAAGTTCTCCAAGGTGGAGGATATGGCAGAGCTGACATGCTTGAACGAAGCCTCTGTCTTACATAATTTGAAGGACCGCTACTATTCAGGACTTATCTATACTTACTCTGGACTCTTCTGTGTGGTGATAAATCCTTACAAGAACCTTCCAATTTACTCTGAGAATATTATTGAAATGTATAGAGGGAAGAAACGCCATGAGATGCCACCACACATCTACGCCATATCAGAGTCTGCTTACAGATGCATGCTTCAAGATCGTGAGGACCAGTCAATTCTATGCACAGGCAATTACTTTTGGGTCCTTCTGTTTACAATGGCCCAGAGAACTGGACTGGAGGATCCCGAGAGGTATCTCTTTGTGGACAGGGCTGTCATCTACAACCCTGCCACTCAAGCTGACTGGACAGCTAAAAAGCTGGTGTGGATTCCATCGGAACGCCATGGTTTTGAGGCAGCTAGTATTAAAGAAGAGCGGGGCGATGAGGTTATGGTGGAGCTGGCAGAGAATGGGAAGAAAGCAATGGTCAACAAAGATGACATTCAGAAGATGAACCCACCAAAGTTCTCCAAGGTGGAGGATATGGCAGAGCTGACATGCTTGAACGAAGCCTCTGTCTTACATAATTTGAAGGACCGCTACTATTCAGGACTTATCTATACTTACTCTGGACTCTTCTGTGTGGTGATAAATCCTTACAAGAACCTTCCAATTTACTCTGAGAATATTATTGAAATGTATAGAGGGAAGAAACGCCATGAGATGCCACCACACATCTACGCCATATCAGAGTCTGCTTACAGATGCATGCTTCAAGATCGTGAGGACCAGTCAATTCTATGC
Peptide sequence:
MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVMVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGNYFWVLLFTMAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVMVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILC
IRESfinder result
IRESfinder score
0.473563237
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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