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mmu_circMyh10_431
Basic information of circular RNA
PanCircBase ID:
mmu_circMyh10_431
mm39_Chr. Position ID:
chr11|68662697|68674330|+
Gene name:
Myh10
Transcript:
ENSMUST00000102611.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
15,16,17
Exon/intron offsets:
0,11234,11511
Genomic length:
11633
Exon/intron sizes in nt:
11,51,94,122
Flanking exon/introns:
chr11:68661883-68662697|chr11:68674330-68675752
Splice length:
431
Other database IDs:
circAtlas: mmu-Myh10_0020
Human Ortholog IDs:
Mature spliced Sequence:
GTGGACTATAAGGCAGATGAGTGGCTGATGAAGAACATGGACCCGCTGAATGACAACGTGGCCACCCTCCTGCACCAGTCCTCGGACAGATTTGTGGCTGAGCTTTGGAAGGACGTGGACCGAATTGTAGGTCTGGATCAAGTCACTGGGATGACTGAGACCGCGTTTGGCTCTGCATACAAAACCAAGAAGGGCATGTTCCGAACCGTCGGGCAGCTCTACAAGGAGTCTCTCACCAAGCTGATGGCAACTCTCCGCAACACCAACCCCAACTTCGTCCGCTGCATCATTCCAAATCACGAGAAGCGGGCTGGGAAACTGGACCCGCACCTCGTGCTCGATCAGCTTCGCTGTAACGGCGTCCTGGAAGGGATCCGGATCTGTCGCCAGGGGTTCCCCAACCGGATAGTTTTCCAGGAATTCAGACAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAAGGGATCCGGATCTGTCG
59.68
AGCCACAAATCTGTCCGAGG
60.04
164
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAAGGGATCCGGATCTGTCG
59.68
GCCACAAATCTGTCCGAGGA
60.04
163
PCR template sequence:
TCGTGCTCGATCAGCTTCGCTGTAACGGCGTCCTGGAAGGGATCCGGATCTGTCGCCAGGGGTTCCCCAACCGGATAGTTTTCCAGGAATTCAGACAGAGGTGGACTATAAGGCAGATGAGTGGCTGATGAAGAACATGGACCCGCTGAATGACAACGTGGCCACCCTCCTGCACCAGTCCTCGGACAGATTTGTGGCTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
AATTCAGACAGAGGTGGACTATAAGG
siRNA sets
ATAGTCCACCTCTGTCTGA,TAGTCCACCTCTGTCTGAA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMYH10_431_ORF_1
1.2302
0.441220425
0.874997329
432
ORF sequence:
ATGAAGAACATGGACCCGCTGAATGACAACGTGGCCACCCTCCTGCACCAGTCCTCGGACAGATTTGTGGCTGAGCTTTGGAAGGACGTGGACCGAATTGTAGGTCTGGATCAAGTCACTGGGATGACTGAGACCGCGTTTGGCTCTGCATACAAAACCAAGAAGGGCATGTTCCGAACCGTCGGGCAGCTCTACAAGGAGTCTCTCACCAAGCTGATGGCAACTCTCCGCAACACCAACCCCAACTTCGTCCGCTGCATCATTCCAAATCACGAGAAGCGGGCTGGGAAACTGGACCCGCACCTCGTGCTCGATCAGCTTCGCTGTAACGGCGTCCTGGAAGGGATCCGGATCTGTCGCCAGGGGTTCCCCAACCGGATAGTTTTCCAGGAATTCAGACAGAGGTGGACTATAAGGCAGATGAGTGGCTGA
Peptide sequence:
MKNMDPLNDNVATLLHQSSDRFVAELWKDVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRWTIRQMSG
IRESfinder result
IRESfinder score
0.274617915
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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