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mmu_circMyef2_72
Basic information of circular RNA
PanCircBase ID:
mmu_circMyef2_72
mm39_Chr. Position ID:
chr2|124939482|124939554|-
Gene name:
Myef2
Transcript:
ENSMUST00000110501.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
13
Exon/intron offsets:
0
Genomic length:
72
Exon/intron sizes in nt:
72
Flanking exon/introns:
chr2:124938040-124939482|chr2:124939554-124939896
Splice length:
72
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GCAGTGCAATGATTGGAGGGTTTGCAGGAAGAATAGGAGCTTCTAACATGGGTCCAGTAGGAACTGGAATAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAGCTTCTAACATGGGTCCAGT
59.76
TCCTGCAAACCCTCCAATCA
59.22
65
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGCTTCTAACATGGGTCCAGT
58.73
TCCTGCAAACCCTCCAATCA
59.22
64
PCR template sequence:
GAGCTTCTAACATGGGTCCAGTAGGAACTGGAATAAGCAGTGCAATGATTGGAGGGTTTGCAGGAAGAATAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
GGAACTGGAATAAGCAGTGCAATGAT
siRNA sets
TCATTGCACTGCTTATTCC, ATCATTGCACTGCTTATTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMYEF2_72_ORF_1
1.2266
-0.122839811
0.076318677
135
ORF sequence:
ATGATTGGAGGGTTTGCAGGAAGAATAGGAGCTTCTAACATGGGTCCAGTAGGAACTGGAATAAGCAGTGCAATGATTGGAGGGTTTGCAGGAAGAATAGGAGCTTCTAACATGGGTCCAGTAGGAACTGGAATA
Peptide sequence:
MIGGFAGRIGASNMGPVGTGISSAMIGGFAGRIGASNMGPVGTGI
IRESfinder result
IRESfinder score
0.568706241
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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