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mmu_circMyef2_240
Basic information of circular RNA
PanCircBase ID:
mmu_circMyef2_240
mm39_Chr. Position ID:
chr2|124939482|124940438|-
Gene name:
Myef2
Transcript:
ENSMUST00000110501.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
13,12,11
Exon/intron offsets:
0,414,887
Genomic length:
956
Exon/intron sizes in nt:
72,99,69
Flanking exon/introns:
chr2:124938040-124939482|chr2:124940438-124942238
Splice length:
240
Other database IDs:
circAtlas: mmu-Myef2_0008
Human Ortholog IDs:
Mature spliced Sequence:
GAGTTGGGTTTGGTGGTCTGGAAGCAATGAATAGCATGGCAGGCTTTGGTGGAGTTGGCCGAATGGGAGAGCTATACCGTGGTGCAATGACTAGTAGCATGGAGCGAGATTTCGGACGTGGTGATATTGGATTAAGTCGAGGCTTTGGCGATTCCTTTGGTAGACTTGGCAGTGCAATGATTGGAGGGTTTGCAGGAAGAATAGGAGCTTCTAACATGGGTCCAGTAGGAACTGGAATAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGCTTTGGCGATTCCTTTGG
60.11
ACCAAAGCCTGCCATGCTAT
60.03
150
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGCTTTGGCGATTCCTTTGG
60.11
ATTCGGCCAACTCCACCAAA
60.18
164
PCR template sequence:
GGCTTTGGCGATTCCTTTGGTAGACTTGGCAGTGCAATGATTGGAGGGTTTGCAGGAAGAATAGGAGCTTCTAACATGGGTCCAGTAGGAACTGGAATAAGAGTTGGGTTTGGTGGTCTGGAAGCAATGAATAGCATGGCAGGCTTTGGTGGAGTTGGCCGAATGGGAGAGCTATACCGTGGTGCAATGACTAGTAGCAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-25-3p(2), mmu-miR-32-5p(1), mmu-miR-363-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GGAACTGGAATAAGAGTTGGGTTTGG
siRNA sets
CAACTCTTATTCCAGTTCC, CAAACCCAACTCTTATTCC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMYEF2_240_ORF_1
1.1406
0.094433197
0.62758702
453
ORF sequence:
ATGAATAGCATGGCAGGCTTTGGTGGAGTTGGCCGAATGGGAGAGCTATACCGTGGTGCAATGACTAGTAGCATGGAGCGAGATTTCGGACGTGGTGATATTGGATTAAGTCGAGGCTTTGGCGATTCCTTTGGTAGACTTGGCAGTGCAATGATTGGAGGGTTTGCAGGAAGAATAGGAGCTTCTAACATGGGTCCAGTAGGAACTGGAATAAGAGTTGGGTTTGGTGGTCTGGAAGCAATGAATAGCATGGCAGGCTTTGGTGGAGTTGGCCGAATGGGAGAGCTATACCGTGGTGCAATGACTAGTAGCATGGAGCGAGATTTCGGACGTGGTGATATTGGATTAAGTCGAGGCTTTGGCGATTCCTTTGGTAGACTTGGCAGTGCAATGATTGGAGGGTTTGCAGGAAGAATAGGAGCTTCTAACATGGGTCCAGTAGGAACTGGAATA
Peptide sequence:
MNSMAGFGGVGRMGELYRGAMTSSMERDFGRGDIGLSRGFGDSFGRLGSAMIGGFAGRIGASNMGPVGTGIRVGFGGLEAMNSMAGFGGVGRMGELYRGAMTSSMERDFGRGDIGLSRGFGDSFGRLGSAMIGGFAGRIGASNMGPVGTGI
IRESfinder result
IRESfinder score
0.295152252
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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