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mmu_circMtx2_132
Basic information of circular RNA
PanCircBase ID:
mmu_circMtx2_132
mm39_Chr. Position ID:
chr2|74698663|74699265|+
Gene name:
Mtx2
Transcript:
ENSMUST00000028511.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
6,7
Exon/intron offsets:
0,563
Genomic length:
602
Exon/intron sizes in nt:
93,39
Flanking exon/introns:
chr2:74697291-74698663|chr2:74699265-74699647
Splice length:
132
Other database IDs:
CIRCpedia: HSA_CIRCpedia_363954
Human Ortholog IDs:
Mature spliced Sequence:
GGCCATTCTCTTAGTGATGGGTTGGATGAAGTCCAAAAAGCAGAAATGAAAGCCTACATGGAATTAGTCAACAATATGCTGCTGACTGCAGAGCTGTATCTCCAGTGGTGTGATGAAGCTACAGTAGGGGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCAACAATATGCTGCTGACTGC
59.58
TCCATGTAGGCTTTCATTTCTGC
59.3
127
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCAACAATATGCTGCTGACTGC
59.58
CCATGTAGGCTTTCATTTCTGCT
59.3
126
PCR template sequence:
GTCAACAATATGCTGCTGACTGCAGAGCTGTATCTCCAGTGGTGTGATGAAGCTACAGTAGGGGAGGGCCATTCTCTTAGTGATGGGTTGGATGAAGTCCAAAAAGCAGAAATGAAAGCCTACATGGAATTA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-15a-5p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-129-5p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
TACAGTAGGGGAGGGCCATTCTCTTA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMTX2_132_ORF_1
1.0677
0.259314719
0.349065251
219
ORF sequence:
ATGAAAGCCTACATGGAATTAGTCAACAATATGCTGCTGACTGCAGAGCTGTATCTCCAGTGGTGTGATGAAGCTACAGTAGGGGAGGGCCATTCTCTTAGTGATGGGTTGGATGAAGTCCAAAAAGCAGAAATGAAAGCCTACATGGAATTAGTCAACAATATGCTGCTGACTGCAGAGCTGTATCTCCAGTGGTGTGATGAAGCTACAGTAGGGGAG
Peptide sequence:
MKAYMELVNNMLLTAELYLQWCDEATVGEGHSLSDGLDEVQKAEMKAYMELVNNMLLTAELYLQWCDEATVGE
IRESfinder result
IRESfinder score
0.322866865
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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