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mmu_circMtr_2515
Basic information of circular RNA
PanCircBase ID:
mmu_circMtr_2515
mm39_Chr. Position ID:
chr13|12212798|12264800|-
Gene name:
Mtr
Transcript:
ENSMUST00000099856.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
24
Exon/intron indexes:
27,26,25,24,23,22,21,20,19,18,17,16,15,14,13,12,11,10,9,8,7,6,5,4
Exon/intron offsets:
0,1133,2284,6830,7377,14475,14758,17500,18897,23509,24242,27549,29771,33032,34989,37544,40068,41736,43652,46022,46642,49406,49980,51932
Genomic length:
52002
Exon/intron sizes in nt:
76,99,82,121,68,101,108,153,90,141,117,180,186,141,113,80,68,62,101,95,63,107,93,70
Flanking exon/introns:
chr13:12210279-12212798|chr13:12264800-12265487
Splice length:
2515
Other database IDs:
circBase: hsa_circ_0112603, circAtlas: hsa-MTR_0130
Human Ortholog IDs:
Mature spliced Sequence:
GCCTACCGGATGAACAAGTGCTCTGCAGATGTGGCCAGAAAAGCCGCTGAGGAGATAACTCTGCAGACAGGAGTCAAGAGGTTTGTGGCTGGAGCTCTGGGTCCGACTAATAAGACACTTTCTGTCTCCCCATCTGTGGAAAGGCCAGATTATAGGAACATCACATTTGATGAGCTTGTTGACGCATACCAGGAGCAGGCCAAGGGACTGCTGGATGGCAGGGTCGACATCTTACTCATTGAAACAATTTTTGATACAGCTAATGCCAAAGCAGCCTTGTTTGCGATCCAGAACCTCTTTGAAGAGAATTATGCTCCTCCTCGGCCTATCTTTATTTCTGGGACCATTGTTGATAAAAGTGGAAGGACTCTTTCTGGGCAGACGGGAGAGGCTTTTGTCACCAGCGTGTCTCATTCGGACCCCCTGTGCATTGGATTAAATTGTTCTCTGGGTGCAGCTGAAATGAGGCCTTTCATTGAAACAATTGGAAAATGTACAACAGCCTATGTCCTCTGTTACCCAAATGCAGGTCTTCCCAACACTTTTGGCGACTATGATGAAACACCATCCACGATGGCCACGCACCTGAAGGACTTTGCTGTGGATGGCTTGGTGAATATAGTTGGTGGATGCTGTGGTTCAACACCTGATCACATCAGGGAAATTGCGGAAGCTGTGAAAAAGTGTAAGCCAAGAGTCCCCCCTGCTAGTGTTTTTGAAGGACATATGTTACTGTCTGGTCTAGAGCCCTTCAGGATTGGACCATACACCAACTTTGTTAACATTGGAGAGCGCTGTAATGTGGCAGGATCCAGGAAATTTGCTAAACTCATCATGGCAGGAAACTATGAAGAAGCCCTGAGCATTGCCAAAGCACAGGTGGAAATGGGAGCTCAGGTGCTCGATATCAACATGGACGACGGCATGCTGGATGGTCCCAGTGCAATGACCAGATTTTGCAACTCCATTGCTTCTGAGCCTGACATTGCCAAGGTGCCCCTGTGCATTGACTCTTCTAACTTTGCCGTGATTGAAGCTGGGTTGAAGTGCTGCCAAGGGAAGTGCATAGTCAACAGCATCAGCCTCAAGGAGGGGGAGGGGGACTTCCTGGAGAAGGCCCGGAAGATTAAGAAGTTTGGAGCTGCTGTGGTGGTTATGGCTTTTGATGAGGAAGGACAAGCAACAGAAACAGATGTTAAAGTCAATGTGTGCACCAGAGCTTACCATCTACTTGTGGACAAAGTGGGCTTCAATCCAAATGACATCATCTTTGACCCCAACATCCTTACCATTGGTACTGGAATGGAGGAACATAACTTGTATGCTATCAATTTTATCCATGCAACAAGAGTCATTAAGGAAACCTTACCAGGAGTCAGAATAAGTGGAGGTCTTTCCAACTTATCCTTCTCCTTCCGAGGGATGGAAGCCATTCGAGAAGCAATGCATGGTGTGTTCCTTTATCACGCGATCAAGTTTGGTATGGACATGGGGATAGTCAATGCCGGCAACCTCCCTGTGTATGATGCTATCCACAAGGACCTTCTTCAGCTCTGTGAAGACCTCATCTGGAACAAAGACTCTGAGGCTACTGAGAAACTCTTGCGATATGCCCAGACTCATGGCACAGGAGGGAAGAAAGTCATCCAGACAGATGAGTGGAGGAATGGCTCCATTGAAGAGCGCCTGGAGTATGCTCTTGTGAAGGGCATTGAAAAGCACATTGTTGAAGATACTGAAGAAGCCAGGTTAAACGGGGAAAAATACCCTCGGCCTCTGAATATAATTGAAGGGCCCCTAATGAATGGCATGAAAGTGGTTGGTGATCTTTTTGGAGCTGGAAAGATGTTTCTACCTCAGGTTATAAAGTCAGCCCGTGTCATGAAGAAGGCTGTTGGCCACCTTATCCCTTTCATGGAAAAAGAAAGAGAAGAAGCCAGATTGATTAATGGTTCGGTTGAAGAAGAGGACCCTTACCAAGGCACCATTGTGCTGGCCACTGTTAAAGGGGACGTGCATGACATCGGCAAGAACATAGTTGGCGTGGTGCTGGCCTGTAACAATTTCAGAGTTATTGATTTAGGAGTCATGACTCCCTGTGATAAGATACTTCAAGCTGCTCTGGACCACAAAGCAGATATAATTGGCTTGTCAGGACTCATCACTCCATCCTTGGATGAGATGATTTTTGTTGCCAAGGAAATGGAGAGGTTGGCTATAAAGATTCCATTGTTGATAGGAGGAGCAACCACTTCAAGAACCCACACAGCAGTTAAAATTGCACCACGGTACAGCGCGCCTGTGATCCACGTCCTAGATGCATCCAAGAGTGTGGTGGTGTGTTCTCAGCTGTTAGATGAAAATCTGAGAGATGACTACTTTGAAGAAATACTGGAAGAGTATGAAGATATTAGACAGGACCACTATGAGTCTCTCAAAGAGAGAAAATACGTACCCCTAAGTCAAGCCAGAAAACACGGTTTCCACATTGATTGGCTCTCTGAACCTCATCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCCAGAAAACACGGTTTCCA
59.26
GCTCCAGCCACAAACCTCTT
60.54
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CGTACCCCTAAGTCAAGCCA
59.1
GCTCCAGCCACAAACCTCTT
60.54
160
PCR template sequence:
CAGGACCACTATGAGTCTCTCAAAGAGAGAAAATACGTACCCCTAAGTCAAGCCAGAAAACACGGTTTCCACATTGATTGGCTCTCTGAACCTCATCCAGGCCTACCGGATGAACAAGTGCTCTGCAGATGTGGCCAGAAAAGCCGCTGAGGAGATAACTCTGCAGACAGGAGTCAAGAGGTTTGTGGCTGGAGCTCTGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
30
Interacting miRNAs (#binding sites)
mmu-miR-136-5p(3), mmu-miR-182-5p(3), mmu-miR-135a-5p(2), mmu-miR-9-5p(1), mmu-miR-140-5p(1), mmu-miR-7b-5p(1), mmu-let-7e-5p(1), mmu-miR-92a-3p(1), mmu-miR-29b-3p(1), mmu-miR-29a-3p(1), mmu-miR-29c-3p(1), mmu-miR-33-5p(1), mmu-miR-298-5p(1), mmu-miR-7a-5p(1), mmu-miR-25-3p(1), mmu-miR-339-5p(1), mmu-miR-434-3p(1), mmu-miR-363-3p(1), mmu-miR-501-3p(1), mmu-miR-383-5p(1), mmu-miR-98-5p(1), mmu-miR-802-5p(1), mmu-miR-207(1), mmu-miR-673-5p(1), mmu-miR-335-3p(1), mmu-miR-484(1), mmu-miR-3065-5p(1), mmu-miR-670-5p(1), mmu-miR-323-3p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GAACCTCATCCAGGCCTACCGGATGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMTR_2515_ORF_1
1.0876
0.229498243
0.999999892
2556
ORF sequence:
ATGAACAAGTGCTCTGCAGATGTGGCCAGAAAAGCCGCTGAGGAGATAACTCTGCAGACAGGAGTCAAGAGGTTTGTGGCTGGAGCTCTGGGTCCGACTAATAAGACACTTTCTGTCTCCCCATCTGTGGAAAGGCCAGATTATAGGAACATCACATTTGATGAGCTTGTTGACGCATACCAGGAGCAGGCCAAGGGACTGCTGGATGGCAGGGTCGACATCTTACTCATTGAAACAATTTTTGATACAGCTAATGCCAAAGCAGCCTTGTTTGCGATCCAGAACCTCTTTGAAGAGAATTATGCTCCTCCTCGGCCTATCTTTATTTCTGGGACCATTGTTGATAAAAGTGGAAGGACTCTTTCTGGGCAGACGGGAGAGGCTTTTGTCACCAGCGTGTCTCATTCGGACCCCCTGTGCATTGGATTAAATTGTTCTCTGGGTGCAGCTGAAATGAGGCCTTTCATTGAAACAATTGGAAAATGTACAACAGCCTATGTCCTCTGTTACCCAAATGCAGGTCTTCCCAACACTTTTGGCGACTATGATGAAACACCATCCACGATGGCCACGCACCTGAAGGACTTTGCTGTGGATGGCTTGGTGAATATAGTTGGTGGATGCTGTGGTTCAACACCTGATCACATCAGGGAAATTGCGGAAGCTGTGAAAAAGTGTAAGCCAAGAGTCCCCCCTGCTAGTGTTTTTGAAGGACATATGTTACTGTCTGGTCTAGAGCCCTTCAGGATTGGACCATACACCAACTTTGTTAACATTGGAGAGCGCTGTAATGTGGCAGGATCCAGGAAATTTGCTAAACTCATCATGGCAGGAAACTATGAAGAAGCCCTGAGCATTGCCAAAGCACAGGTGGAAATGGGAGCTCAGGTGCTCGATATCAACATGGACGACGGCATGCTGGATGGTCCCAGTGCAATGACCAGATTTTGCAACTCCATTGCTTCTGAGCCTGACATTGCCAAGGTGCCCCTGTGCATTGACTCTTCTAACTTTGCCGTGATTGAAGCTGGGTTGAAGTGCTGCCAAGGGAAGTGCATAGTCAACAGCATCAGCCTCAAGGAGGGGGAGGGGGACTTCCTGGAGAAGGCCCGGAAGATTAAGAAGTTTGGAGCTGCTGTGGTGGTTATGGCTTTTGATGAGGAAGGACAAGCAACAGAAACAGATGTTAAAGTCAATGTGTGCACCAGAGCTTACCATCTACTTGTGGACAAAGTGGGCTTCAATCCAAATGACATCATCTTTGACCCCAACATCCTTACCATTGGTACTGGAATGGAGGAACATAACTTGTATGCTATCAATTTTATCCATGCAACAAGAGTCATTAAGGAAACCTTACCAGGAGTCAGAATAAGTGGAGGTCTTTCCAACTTATCCTTCTCCTTCCGAGGGATGGAAGCCATTCGAGAAGCAATGCATGGTGTGTTCCTTTATCACGCGATCAAGTTTGGTATGGACATGGGGATAGTCAATGCCGGCAACCTCCCTGTGTATGATGCTATCCACAAGGACCTTCTTCAGCTCTGTGAAGACCTCATCTGGAACAAAGACTCTGAGGCTACTGAGAAACTCTTGCGATATGCCCAGACTCATGGCACAGGAGGGAAGAAAGTCATCCAGACAGATGAGTGGAGGAATGGCTCCATTGAAGAGCGCCTGGAGTATGCTCTTGTGAAGGGCATTGAAAAGCACATTGTTGAAGATACTGAAGAAGCCAGGTTAAACGGGGAAAAATACCCTCGGCCTCTGAATATAATTGAAGGGCCCCTAATGAATGGCATGAAAGTGGTTGGTGATCTTTTTGGAGCTGGAAAGATGTTTCTACCTCAGGTTATAAAGTCAGCCCGTGTCATGAAGAAGGCTGTTGGCCACCTTATCCCTTTCATGGAAAAAGAAAGAGAAGAAGCCAGATTGATTAATGGTTCGGTTGAAGAAGAGGACCCTTACCAAGGCACCATTGTGCTGGCCACTGTTAAAGGGGACGTGCATGACATCGGCAAGAACATAGTTGGCGTGGTGCTGGCCTGTAACAATTTCAGAGTTATTGATTTAGGAGTCATGACTCCCTGTGATAAGATACTTCAAGCTGCTCTGGACCACAAAGCAGATATAATTGGCTTGTCAGGACTCATCACTCCATCCTTGGATGAGATGATTTTTGTTGCCAAGGAAATGGAGAGGTTGGCTATAAAGATTCCATTGTTGATAGGAGGAGCAACCACTTCAAGAACCCACACAGCAGTTAAAATTGCACCACGGTACAGCGCGCCTGTGATCCACGTCCTAGATGCATCCAAGAGTGTGGTGGTGTGTTCTCAGCTGTTAGATGAAAATCTGAGAGATGACTACTTTGAAGAAATACTGGAAGAGTATGAAGATATTAGACAGGACCACTATGAGTCTCTCAAAGAGAGAAAATACGTACCCCTAAGTCAAGCCAGAAAACACGGTTTCCACATTGATTGGCTCTCTGAACCTCATCCAGGCCTACCGGATGAACAAGTGCTCTGCAGATGTGGCCAGAAAAGCCGCTGA
Peptide sequence:
MNKCSADVARKAAEEITLQTGVKRFVAGALGPTNKTLSVSPSVERPDYRNITFDELVDAYQEQAKGLLDGRVDILLIETIFDTANAKAALFAIQNLFEENYAPPRPIFISGTIVDKSGRTLSGQTGEAFVTSVSHSDPLCIGLNCSLGAAEMRPFIETIGKCTTAYVLCYPNAGLPNTFGDYDETPSTMATHLKDFAVDGLVNIVGGCCGSTPDHIREIAEAVKKCKPRVPPASVFEGHMLLSGLEPFRIGPYTNFVNIGERCNVAGSRKFAKLIMAGNYEEALSIAKAQVEMGAQVLDINMDDGMLDGPSAMTRFCNSIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEGEGDFLEKARKIKKFGAAVVVMAFDEEGQATETDVKVNVCTRAYHLLVDKVGFNPNDIIFDPNILTIGTGMEEHNLYAINFIHATRVIKETLPGVRISGGLSNLSFSFRGMEAIREAMHGVFLYHAIKFGMDMGIVNAGNLPVYDAIHKDLLQLCEDLIWNKDSEATEKLLRYAQTHGTGGKKVIQTDEWRNGSIEERLEYALVKGIEKHIVEDTEEARLNGEKYPRPLNIIEGPLMNGMKVVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKEREEARLINGSVEEEDPYQGTIVLATVKGDVHDIGKNIVGVVLACNNFRVIDLGVMTPCDKILQAALDHKADIIGLSGLITPSLDEMIFVAKEMERLAIKIPLLIGGATTSRTHTAVKIAPRYSAPVIHVLDASKSVVVCSQLLDENLRDDYFEEILEEYEDIRQDHYESLKERKYVPLSQARKHGFHIDWLSEPHPGLPDEQVLCRCGQKSR
IRESfinder result
IRESfinder score
0.504384234
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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