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mmu_circMtif2_1367
Basic information of circular RNA
PanCircBase ID:
mmu_circMtif2_1367
mm39_Chr. Position ID:
chr11|29483831|29492509|+
Gene name:
Mtif2
Transcript:
ENSMUST00000020749.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
5,6,7,8,9,10,11,12
Exon/intron offsets:
0,1258,3001,4837,6236,6777,7509,8513
Genomic length:
8678
Exon/intron sizes in nt:
1,61,17,71,40,12,52,05,00,00,00,000
Flanking exon/introns:
chr11:29483397-29483831|chr11:29492509-29494269
Splice length:
1367
Other database IDs:
circAtlas: mmu-Mtif2_0019
Human Ortholog IDs:
Mature spliced Sequence:
GCCTGGGACAGATCCAGCTTTATTAAAACCAAGGTCCCCAGTTGTTACTGTAATGGGCCATGTTGATCATGGGAAAACGACCTTACTTGACAAACTTCGAGAAACTCAAGTTGCAGCGATGGAAGTCGGAGGCATCACTCAACACATTGGTGCTTTTCTTGTCTCTCTGCCTTCTGGAGAAAAGATAACTTTTCTTGATACTCCTGGACATGCTGCCTTCTCAGCAATGAGAGCCAGAGGAGCTCAGGTCACCGACATTGTTGTGTTGGTTGTAGCTGCAGATGATGGGGTAATGAAACAAACTGTGGAATCCATTCAGCATGCAAAAGATGCAGAAGTTCCTATTATCCTTGCAATCAATAAGTGCGACAAGACAGATGCTGATCCTGAAAAGGTGAAAAAAGAGCTCCTAGCTTACGATGTGGTGTGTGAAGAGTATGGTGGTGATGTTCAAGCAGTGCACGTCTCTGCACTTACGGGCGATAACCTGATGGCTTTGGCAGAAGCAACAATTGCTCTCGCAGAAATCTTGGAACTGAAAGCAGATCCCACCGGTCCAGTGGAAGGAACAGTAATAGAGTCTTTCACAGACAAAGGAAGAGGTCCTGTTACAACAGCTATAATTCAAAGAGGAACTCTGAGAAAAGGCTCAATTCTAGTTGCTGGGAAGAGTTGGGCAAAAGTTCGACTAATATTTGATGAAAATGGAAAAATACTTAATGAGGCCTATCCCAGCATGCCAGTGGGAATCATAGGCTGGAGAGACCTTCCTTCTGCAGGAGATGAAATTCTTGAAGTAGAATCTGAGCCAAGGGCCCGTGAAGTTATTGAATGGAGGAAGTCTGAGCAAAAAGAAGAAAAAGGCAAAGATGACCTGAAAATAATGGAAGAAAAGCGAAGGGAACACCAAGAAGCGCATCGGAAAGCCCGTGAGAAGTATGGCAGTCTGCACTGGAAAGAGAGATCATATATAAAGTTCCTTGAAAGGAAACAACAGAGACCCTTAAAGCCCAAAGAAAAGGTAGAAAGGCAATCAAATGTACTTCCTATAATTATTAAAGGTGACGTTGATGGGTCTGTGGAGGCCATCTTGAACCTTCTGGATACCTACGATGCTTCCCACGAATGTGAACTCGAATTAGTACATTTTGGATTGGGTGACATCAGTGAAAATGATGTTACCTTTGCTGAGACATTTGATGGTGTTATTTATGGTTTTAATGTGGAGGCAGGCAGTGCTATTCAGCAGTCAGCTGCACAAAAGGGAGTTAAGATTAAACTTCACAAAATCATCTACCATCTTATTGAAGATTTGCAGGAGGAACTAAGCAGCAGATTGCCCCACACACTGGAGGAGTACCCAATAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCAGGAGGAACTAAGCAGCA
60.04
TGTCAAGTAAGGTCGTTTTCCCA
60.12
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGCAGGAGGAACTAAGCAGC
60.04
TGTCAAGTAAGGTCGTTTTCCCA
60.12
146
PCR template sequence:
GTTAAGATTAAACTTCACAAAATCATCTACCATCTTATTGAAGATTTGCAGGAGGAACTAAGCAGCAGATTGCCCCACACACTGGAGGAGTACCCAATAGGCCTGGGACAGATCCAGCTTTATTAAAACCAAGGTCCCCAGTTGTTACTGTAATGGGCCATGTTGATCATGGGAAAACGACCTTACTTGACAAACTTCGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
14
Interacting miRNAs (#binding sites)
mmu-miR-23b-3p(1), mmu-miR-199a-5p(1), mmu-miR-103-3p(1), mmu-miR-29b-3p(1), mmu-miR-152-3p(1), mmu-miR-148a-3p(1), mmu-miR-320-3p(1), mmu-miR-23a-3p(1), mmu-miR-24-3p(1), mmu-miR-194-5p(1), mmu-miR-467b-5p(1), mmu-miR-107-3p(1), mmu-miR-802-5p(1), mmu-miR-129-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GAGTACCCAATAGGCCTGGGACAGAT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMTIF2_1367_ORF_1
1.0546
0.124057201
0.998929435
1341
ORF sequence:
ATGGGCCATGTTGATCATGGGAAAACGACCTTACTTGACAAACTTCGAGAAACTCAAGTTGCAGCGATGGAAGTCGGAGGCATCACTCAACACATTGGTGCTTTTCTTGTCTCTCTGCCTTCTGGAGAAAAGATAACTTTTCTTGATACTCCTGGACATGCTGCCTTCTCAGCAATGAGAGCCAGAGGAGCTCAGGTCACCGACATTGTTGTGTTGGTTGTAGCTGCAGATGATGGGGTAATGAAACAAACTGTGGAATCCATTCAGCATGCAAAAGATGCAGAAGTTCCTATTATCCTTGCAATCAATAAGTGCGACAAGACAGATGCTGATCCTGAAAAGGTGAAAAAAGAGCTCCTAGCTTACGATGTGGTGTGTGAAGAGTATGGTGGTGATGTTCAAGCAGTGCACGTCTCTGCACTTACGGGCGATAACCTGATGGCTTTGGCAGAAGCAACAATTGCTCTCGCAGAAATCTTGGAACTGAAAGCAGATCCCACCGGTCCAGTGGAAGGAACAGTAATAGAGTCTTTCACAGACAAAGGAAGAGGTCCTGTTACAACAGCTATAATTCAAAGAGGAACTCTGAGAAAAGGCTCAATTCTAGTTGCTGGGAAGAGTTGGGCAAAAGTTCGACTAATATTTGATGAAAATGGAAAAATACTTAATGAGGCCTATCCCAGCATGCCAGTGGGAATCATAGGCTGGAGAGACCTTCCTTCTGCAGGAGATGAAATTCTTGAAGTAGAATCTGAGCCAAGGGCCCGTGAAGTTATTGAATGGAGGAAGTCTGAGCAAAAAGAAGAAAAAGGCAAAGATGACCTGAAAATAATGGAAGAAAAGCGAAGGGAACACCAAGAAGCGCATCGGAAAGCCCGTGAGAAGTATGGCAGTCTGCACTGGAAAGAGAGATCATATATAAAGTTCCTTGAAAGGAAACAACAGAGACCCTTAAAGCCCAAAGAAAAGGTAGAAAGGCAATCAAATGTACTTCCTATAATTATTAAAGGTGACGTTGATGGGTCTGTGGAGGCCATCTTGAACCTTCTGGATACCTACGATGCTTCCCACGAATGTGAACTCGAATTAGTACATTTTGGATTGGGTGACATCAGTGAAAATGATGTTACCTTTGCTGAGACATTTGATGGTGTTATTTATGGTTTTAATGTGGAGGCAGGCAGTGCTATTCAGCAGTCAGCTGCACAAAAGGGAGTTAAGATTAAACTTCACAAAATCATCTACCATCTTATTGAAGATTTGCAGGAGGAACTAAGCAGCAGATTGCCCCACACACTGGAGGAGTACCCAATAGGCCTGGGACAGATCCAGCTTTATTAA
Peptide sequence:
MGHVDHGKTTLLDKLRETQVAAMEVGGITQHIGAFLVSLPSGEKITFLDTPGHAAFSAMRARGAQVTDIVVLVVAADDGVMKQTVESIQHAKDAEVPIILAINKCDKTDADPEKVKKELLAYDVVCEEYGGDVQAVHVSALTGDNLMALAEATIALAEILELKADPTGPVEGTVIESFTDKGRGPVTTAIIQRGTLRKGSILVAGKSWAKVRLIFDENGKILNEAYPSMPVGIIGWRDLPSAGDEILEVESEPRAREVIEWRKSEQKEEKGKDDLKIMEEKRREHQEAHRKAREKYGSLHWKERSYIKFLERKQQRPLKPKEKVERQSNVLPIIIKGDVDGSVEAILNLLDTYDASHECELELVHFGLGDISENDVTFAETFDGVIYGFNVEAGSAIQQSAAQKGVKIKLHKIIYHLIEDLQEELSSRLPHTLEEYPIGLGQIQLY
IRESfinder result
IRESfinder score
0.548396353
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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