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mmu_circMroh1_1295
Basic information of circular RNA
PanCircBase ID:
mmu_circMroh1_1295
mm39_Chr. Position ID:
chr15|76274230|76298532|+
Gene name:
Mroh1
Transcript:
ENSMUST00000161305.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
11
Exon/intron indexes:
2,3,4,5,6,7,8,9,10,11,12
Exon/intron offsets:
0,2350,3772,11545,12102,12452,17861,18357,18852,22871,24188
Genomic length:
24302
Exon/intron sizes in nt:
9,77,91,46,13,21,63,99,00,00,00,00,00,000
Flanking exon/introns:
chr15:76264542-76274230|chr15:76298532-76305339
Splice length:
1295
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATGCTCTCCTGGACGGCGGTTGGGATGCTGTGCTGATGCTGCGGTCCTCAAGGCCCTCTGCCTGCCTTCTGTCCTCTCGGCCACCTGGGACTTCCTGGCTGGGGAGGAAGCTATCCATGCTCACACTGGACAGGCGGAAGCTGAGAGCACAGAAATGACCAGGCCCTACATAAAGAGGCTGTCCTTCACCCTCCTGGACTCCATCACTGACAAGGATCCTATGGTCCAGGAGCAGGTCTGCAGTGCACTCTGTTCCCTTGGAGACGCGCAGCCAGACGAGACACTCCATGCCTGTGAGGAGTACCTACGGCAGCATGACAAGCTGGCTCACCCGTACCGTACGAAGATCCTGAGGGCCATGGAGACAGTACTAAGCAGTCATATCCATGACCTGGACAAGGACACTGCCGGCGCTGTCATCCTCCTGGCTACCAGCGAGATGACAAGGACCAAGGAGCTGGACTGTGACTGGCAGCAGGCTGCAGGCAGTGTCCTGGTGGCTGTTGGCAAGCGATTCACCAACCAGGTGATGGAGGAGGTGCTCAGCCGGTTCCAGCCTGGGATGCTGCCCCACTCCTCCGTGCTGCACACACTCGCCAACCTCTCAGTGTCCAACGCATTTGATATGGTTCCTTTTCTGCCGTCCATCCTGAGCACCATGCTGCCTATGCTGAGCATGGCCAAGCAGGACGCGCTGAAGGTGGTGTTCTGCGGCGCTCTCCAGCACTTCAGCGAAAGCATCTTGGAATACCTGGCCAACCTGGACCAGGCCCCAGACCCCACAGTGAGGAAGGACACCTTTGGGGCTGACATCTTTGGCGCCTATGATGTTCTCTTCCACCACTGGCTGCAGAGCCGAGATGCCAAGCTCCGGCTTGCCGTGGTGGCGGCTCTGGGTCCCATGAGCCACCTGCTTCCCAGTGAGAGGCTGGAAGAACAGCTCCCTAAGCTCCTCCCTGCAGTCCTCGGCCTCTACAAGAAGCATGCGGAGGCCTTTCAGATATCGAAGAGCCTGGGCCAGATTCTCGAGGCAGCTGTGAACGTGAGCAGCCGTACCCTGGAGGTTCAGCTTGATGCCCTCCTGGTGGCTCTTCATGCTCAGATTTGTGTGCCTGTGGAGTCCTCGAGCCCCCTGGTGATGAACAGCCAGAAGGAGGTGCTTCGCTGCTTCACAGTGCTGGCCTGCTGCTCTCCTGACCGTCTGCTGGCCTTCTTACTGCCCAGGCTGGACACCAGCAACGAGAGGCTCCGCGTGGGTACCCTGCAGATCCTGAGACACATCATCAACTCGGCTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCTGCTGGCCTTCTTACTGC
60.04
GACAGAAGGCAGGCAGAGG
60.08
168
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCTGCTGGCCTTCTTACTGC
60.04
GGACAGAAGGCAGGCAGAG
60.08
169
PCR template sequence:
GACCGTCTGCTGGCCTTCTTACTGCCCAGGCTGGACACCAGCAACGAGAGGCTCCGCGTGGGTACCCTGCAGATCCTGAGACACATCATCAACTCGGCTGATGCTCTCCTGGACGGCGGTTGGGATGCTGTGCTGATGCTGCGGTCCTCAAGGCCCTCTGCCTGCCTTCTGTCCTCTCGGCCACCTGGGACTTCCTGGCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
18
Interacting miRNAs (#binding sites)
mmu-miR-103-3p(2), mmu-miR-107-3p(2), mmu-miR-16-5p(1), mmu-miR-27a-3p(1), mmu-miR-150-5p(1), mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-322-5p(1), mmu-miR-148a-3p(1), mmu-miR-214-3p(1), mmu-miR-503-5p(1), mmu-miR-149-5p(1), mmu-miR-22-3p(1), mmu-miR-212-3p(1), mmu-miR-324-3p(1), mmu-miR-34b-5p(1), mmu-miR-574-5p(1), mmu-miR-339-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ATCAACTCGGCTGATGCTCTCCTGGA
siRNA sets
TCCAGGAGAGCATCAGCCG, CAGGAGAGCATCAGCCGAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMROH1_1295_ORF_1
1.2694
0.430404384
0.999722871
1299
ORF sequence:
ATGACCAGGCCCTACATAAAGAGGCTGTCCTTCACCCTCCTGGACTCCATCACTGACAAGGATCCTATGGTCCAGGAGCAGGTCTGCAGTGCACTCTGTTCCCTTGGAGACGCGCAGCCAGACGAGACACTCCATGCCTGTGAGGAGTACCTACGGCAGCATGACAAGCTGGCTCACCCGTACCGTACGAAGATCCTGAGGGCCATGGAGACAGTACTAAGCAGTCATATCCATGACCTGGACAAGGACACTGCCGGCGCTGTCATCCTCCTGGCTACCAGCGAGATGACAAGGACCAAGGAGCTGGACTGTGACTGGCAGCAGGCTGCAGGCAGTGTCCTGGTGGCTGTTGGCAAGCGATTCACCAACCAGGTGATGGAGGAGGTGCTCAGCCGGTTCCAGCCTGGGATGCTGCCCCACTCCTCCGTGCTGCACACACTCGCCAACCTCTCAGTGTCCAACGCATTTGATATGGTTCCTTTTCTGCCGTCCATCCTGAGCACCATGCTGCCTATGCTGAGCATGGCCAAGCAGGACGCGCTGAAGGTGGTGTTCTGCGGCGCTCTCCAGCACTTCAGCGAAAGCATCTTGGAATACCTGGCCAACCTGGACCAGGCCCCAGACCCCACAGTGAGGAAGGACACCTTTGGGGCTGACATCTTTGGCGCCTATGATGTTCTCTTCCACCACTGGCTGCAGAGCCGAGATGCCAAGCTCCGGCTTGCCGTGGTGGCGGCTCTGGGTCCCATGAGCCACCTGCTTCCCAGTGAGAGGCTGGAAGAACAGCTCCCTAAGCTCCTCCCTGCAGTCCTCGGCCTCTACAAGAAGCATGCGGAGGCCTTTCAGATATCGAAGAGCCTGGGCCAGATTCTCGAGGCAGCTGTGAACGTGAGCAGCCGTACCCTGGAGGTTCAGCTTGATGCCCTCCTGGTGGCTCTTCATGCTCAGATTTGTGTGCCTGTGGAGTCCTCGAGCCCCCTGGTGATGAACAGCCAGAAGGAGGTGCTTCGCTGCTTCACAGTGCTGGCCTGCTGCTCTCCTGACCGTCTGCTGGCCTTCTTACTGCCCAGGCTGGACACCAGCAACGAGAGGCTCCGCGTGGGTACCCTGCAGATCCTGAGACACATCATCAACTCGGCTGATGCTCTCCTGGACGGCGGTTGGGATGCTGTGCTGATGCTGCGGTCCTCAAGGCCCTCTGCCTGCCTTCTGTCCTCTCGGCCACCTGGGACTTCCTGGCTGGGGAGGAAGCTATCCATGCTCACACTGGACAGGCGGAAGCTGAGAGCACAGAAATGA
Peptide sequence:
MTRPYIKRLSFTLLDSITDKDPMVQEQVCSALCSLGDAQPDETLHACEEYLRQHDKLAHPYRTKILRAMETVLSSHIHDLDKDTAGAVILLATSEMTRTKELDCDWQQAAGSVLVAVGKRFTNQVMEEVLSRFQPGMLPHSSVLHTLANLSVSNAFDMVPFLPSILSTMLPMLSMAKQDALKVVFCGALQHFSESILEYLANLDQAPDPTVRKDTFGADIFGAYDVLFHHWLQSRDAKLRLAVVAALGPMSHLLPSERLEEQLPKLLPAVLGLYKKHAEAFQISKSLGQILEAAVNVSSRTLEVQLDALLVALHAQICVPVESSSPLVMNSQKEVLRCFTVLACCSPDRLLAFLLPRLDTSNERLRVGTLQILRHIINSADALLDGGWDAVLMLRSSRPSACLLSSRPPGTSWLGRKLSMLTLDRRKLRAQK
IRESfinder result
IRESfinder score
0.286231624
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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