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mmu_circMprip_796
Basic information of circular RNA
PanCircBase ID:
mmu_circMprip_796
mm39_Chr. Position ID:
chr11|59655205|59660872|+
Gene name:
Mprip
Transcript:
ENSMUST00000072031.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
16,17,18,19,20,21
Exon/intron offsets:
0,1028,2348,3575,4833,5504
Genomic length:
5667
Exon/intron sizes in nt:
14,81,18,16,01,20,87,100
Flanking exon/introns:
chr11:59645935-59655205|chr11:59660872-59662442
Splice length:
796
Other database IDs:
CIRCpedia: MMU_CIRCpedia_229807, circAtlas: mmu-Mprip_0008
circBase: hsa_circ_0042237, CIRCpedia: HSA_CIRCpedia_19458
Human Ortholog IDs:
Mature spliced Sequence:
GCTACATGTGAGCGAGGCTTTGCCGCCATGGAGGAGACACATCAGAAGAAGATTGAAGACCTACAGAGGCAACACCAGCGGGAGCTAGAGAAGCTGCGGGAGGAAAAAGACCGCCTCCTGGCTGAGGAGACAGCTGCCACCATATCAGCCATTGAAGCCATGAAGAATGCTCACCGAGAGGAGATGGAACGAGAGTTAGAGAAGAGCCAGCGGTCTCAGATCAGCAGCATCAATTCAGACATAGAAGCCCTGAGGCGACAGTACCTGGAGGAGCTGCAGTCAGTGCAGCGGGAGCTGGAAGTCCTTTCAGAGCAGTACTCCCAAAAGTGCCTGGAGAATGCACACCTAGCCCAGGCGCTGGAGGCCGAGCGTCAGGCCCTGCGGCAATGCCAGCGTGAGAACCAGGAGCTCAACGCCCACAACCAGGAGCTGAATAACCGCCTGGCTGCGGAGATCACACGGTTGCGGACACTACTGACTGGAGACGGTGGTGGGGAGTCCACTGGGTTGCCTCTCACACAGGGCAAGGATGCCTATGAGTTAGAGGTCTTGTTGCGGGTCAAAGAGTCAGAAATCCAGTACCTGAAACAGGAGATCAGCTCCCTCAAGGATGAACTCCAGACAGCCCTGCGGGATAAGAAGTATGCTAGTGACAAGTACAAAGACATCTACACTGAGCTCAGCATTGCCAAGGCCAAGGCTGACTGCGACATCAGCAGGCTGAAGGAGCAGCTGAAAGCAGCCACAGAGGCACTGGGCGAGAAATCTCCTGAAGGCACTACTGTGTCAGGATATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AAGGCTGACTGCGACATCAG
60.39
GATGTGTCTCCTCCATGGCG
60.53
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAGGCTGACTGCGACATCAG
60.39
TGATGTGTCTCCTCCATGGC
59.46
144
PCR template sequence:
AAGGCTGACTGCGACATCAGCAGGCTGAAGGAGCAGCTGAAAGCAGCCACAGAGGCACTGGGCGAGAAATCTCCTGAAGGCACTACTGTGTCAGGATATGGCTACATGTGAGCGAGGCTTTGCCGCCATGGAGGAGACACATCAGAAGAAGATTGAAGACCTACAGAGGCAACACCAGCGGGAGCTAGAGAAGCTGCGGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-138-5p(2), mmu-miR-135a-5p(1), mmu-miR-149-5p(1), mmu-miR-96-5p(1), mmu-miR-199b-3p(1), mmu-miR-182-5p(1), mmu-miR-199a-3p(1), mmu-miR-370-3p(1), mmu-miR-362-5p(1), mmu-miR-697(1)
siRNAs for circular RNA silencing
Junction sequence
GTGTCAGGATATGGCTACATGTGAGC
siRNA sets
CTCACATGTAGCCATATCC, TCACATGTAGCCATATCCT, CACATGTAGCCATATCCTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMPRIP_796_ORF_1
1.1964
0.580593263
0.993799927
798
ORF sequence:
ATGGCTACATGTGAGCGAGGCTTTGCCGCCATGGAGGAGACACATCAGAAGAAGATTGAAGACCTACAGAGGCAACACCAGCGGGAGCTAGAGAAGCTGCGGGAGGAAAAAGACCGCCTCCTGGCTGAGGAGACAGCTGCCACCATATCAGCCATTGAAGCCATGAAGAATGCTCACCGAGAGGAGATGGAACGAGAGTTAGAGAAGAGCCAGCGGTCTCAGATCAGCAGCATCAATTCAGACATAGAAGCCCTGAGGCGACAGTACCTGGAGGAGCTGCAGTCAGTGCAGCGGGAGCTGGAAGTCCTTTCAGAGCAGTACTCCCAAAAGTGCCTGGAGAATGCACACCTAGCCCAGGCGCTGGAGGCCGAGCGTCAGGCCCTGCGGCAATGCCAGCGTGAGAACCAGGAGCTCAACGCCCACAACCAGGAGCTGAATAACCGCCTGGCTGCGGAGATCACACGGTTGCGGACACTACTGACTGGAGACGGTGGTGGGGAGTCCACTGGGTTGCCTCTCACACAGGGCAAGGATGCCTATGAGTTAGAGGTCTTGTTGCGGGTCAAAGAGTCAGAAATCCAGTACCTGAAACAGGAGATCAGCTCCCTCAAGGATGAACTCCAGACAGCCCTGCGGGATAAGAAGTATGCTAGTGACAAGTACAAAGACATCTACACTGAGCTCAGCATTGCCAAGGCCAAGGCTGACTGCGACATCAGCAGGCTGAAGGAGCAGCTGAAAGCAGCCACAGAGGCACTGGGCGAGAAATCTCCTGAAGGCACTACTGTGTCAGGATAT
Peptide sequence:
MATCERGFAAMEETHQKKIEDLQRQHQRELEKLREEKDRLLAEETAATISAIEAMKNAHREEMERELEKSQRSQISSINSDIEALRRQYLEELQSVQRELEVLSEQYSQKCLENAHLAQALEAERQALRQCQRENQELNAHNQELNNRLAAEITRLRTLLTGDGGGESTGLPLTQGKDAYELEVLLRVKESEIQYLKQEISSLKDELQTALRDKKYASDKYKDIYTELSIAKAKADCDISRLKEQLKAATEALGEKSPEGTTVSGY
IRESfinder result
IRESfinder score
0.248066842
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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