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mmu_circMprip_771
Basic information of circular RNA
PanCircBase ID:
mmu_circMprip_771
mm39_Chr. Position ID:
chr11|59639372|59643886|+
Gene name:
Mprip
Transcript:
ENSMUST00000072031.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
12,13,14
Exon/intron offsets:
0,972,3943
Genomic length:
4514
Exon/intron sizes in nt:
9,01,10,571
Flanking exon/introns:
chr11:59635646-59639372|chr11:59643886-59645818
Splice length:
771
Other database IDs:
circAtlas: mmu-Mprip_0046
Human Ortholog IDs:
Mature spliced Sequence:
GCAGCTGACCTAGATGGAGAAATTAACTTGTCCACTTGCTATGATGTCACTGAGTATCCAGTCCAGAGAAACTATGGCTTTCAGATACATACCAAGGAGGGTGAGTTCACCCTCTCAGCCATGACATCTGGCATACGACGGAACTGGATCCAGACCATTATGAAACATGTGCTCCCAGCATCTGCCCCAGATGTGACCAGCTCATTGCCTGAGGGAAAGAATAAAAGCACATCTTTTGAGACCTGCTCAAGGTCAACTGAGAAGCAAGAAGCTGAGCCAGGAGAGCCAGATCCTGAACAAAAGAAGAGCCGTGCTCGAGAGCGGAGGCGGGAGGGTCGCTCCAAGACCTTTGACTGGGCTGAATTCCGCCCTATACAACAGGCCCTGGCTCAAGAAAGGGCCAGCGCTGTTGGGTCTTCTGATTCTGGTGACCCTGGGTGTCTTGAAGCCGAGCCAGGTGAGCTGGAGCGTGAACGAGCTCGACGTCGAGAAGAACGCCGCAAGCGCTTTGGGATGCTGGACACCATTGATGGACCAGGAATGGAGGACACAGCCCTACGTATGGACATCGACCGGAGCCCAGGGCTGCTGGGAACCCCTGACCTCAAGACACAGAATGTCCACGTGGAAATTGAGCAGCGTTGGCATCAAGTGGAGACCACCCCTCTCAGGGAGGAGAAACAGGTGCCCATTGCCCCTCTGCACCTATCCTTGGAGGACAGAAGTGAGCGGCTCTCCACACATGAGCTCACCTCTCTGCTAGAGAAGGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CATTGCCCCTCTGCACCTAT
59.82
GCCATAGTTTCTCTGGACTGGA
59.5
160
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CATTGCCCCTCTGCACCTAT
59.82
AGCCATAGTTTCTCTGGACTGG
59.5
161
PCR template sequence:
GGAGGAGAAACAGGTGCCCATTGCCCCTCTGCACCTATCCTTGGAGGACAGAAGTGAGCGGCTCTCCACACATGAGCTCACCTCTCTGCTAGAGAAGGAGGCAGCTGACCTAGATGGAGAAATTAACTTGTCCACTTGCTATGATGTCACTGAGTATCCAGTCCAGAGAAACTATGGCTTTCAGATACATACCAAGGAGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-149-5p(2), mmu-miR-328-3p(1), mmu-miR-324-5p(1), mmu-miR-136-5p(1), mmu-miR-3087-3p(1), mmu-miR-670-5p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GCTAGAGAAGGAGGCAGCTGACCTAG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMPRIP_771_ORF_1
1.0468
0.373644345
0.999787595
1422
ORF sequence:
ATGACATCTGGCATACGACGGAACTGGATCCAGACCATTATGAAACATGTGCTCCCAGCATCTGCCCCAGATGTGACCAGCTCATTGCCTGAGGGAAAGAATAAAAGCACATCTTTTGAGACCTGCTCAAGGTCAACTGAGAAGCAAGAAGCTGAGCCAGGAGAGCCAGATCCTGAACAAAAGAAGAGCCGTGCTCGAGAGCGGAGGCGGGAGGGTCGCTCCAAGACCTTTGACTGGGCTGAATTCCGCCCTATACAACAGGCCCTGGCTCAAGAAAGGGCCAGCGCTGTTGGGTCTTCTGATTCTGGTGACCCTGGGTGTCTTGAAGCCGAGCCAGGTGAGCTGGAGCGTGAACGAGCTCGACGTCGAGAAGAACGCCGCAAGCGCTTTGGGATGCTGGACACCATTGATGGACCAGGAATGGAGGACACAGCCCTACGTATGGACATCGACCGGAGCCCAGGGCTGCTGGGAACCCCTGACCTCAAGACACAGAATGTCCACGTGGAAATTGAGCAGCGTTGGCATCAAGTGGAGACCACCCCTCTCAGGGAGGAGAAACAGGTGCCCATTGCCCCTCTGCACCTATCCTTGGAGGACAGAAGTGAGCGGCTCTCCACACATGAGCTCACCTCTCTGCTAGAGAAGGAGGCAGCTGACCTAGATGGAGAAATTAACTTGTCCACTTGCTATGATGTCACTGAGTATCCAGTCCAGAGAAACTATGGCTTTCAGATACATACCAAGGAGGGTGAGTTCACCCTCTCAGCCATGACATCTGGCATACGACGGAACTGGATCCAGACCATTATGAAACATGTGCTCCCAGCATCTGCCCCAGATGTGACCAGCTCATTGCCTGAGGGAAAGAATAAAAGCACATCTTTTGAGACCTGCTCAAGGTCAACTGAGAAGCAAGAAGCTGAGCCAGGAGAGCCAGATCCTGAACAAAAGAAGAGCCGTGCTCGAGAGCGGAGGCGGGAGGGTCGCTCCAAGACCTTTGACTGGGCTGAATTCCGCCCTATACAACAGGCCCTGGCTCAAGAAAGGGCCAGCGCTGTTGGGTCTTCTGATTCTGGTGACCCTGGGTGTCTTGAAGCCGAGCCAGGTGAGCTGGAGCGTGAACGAGCTCGACGTCGAGAAGAACGCCGCAAGCGCTTTGGGATGCTGGACACCATTGATGGACCAGGAATGGAGGACACAGCCCTACGTATGGACATCGACCGGAGCCCAGGGCTGCTGGGAACCCCTGACCTCAAGACACAGAATGTCCACGTGGAAATTGAGCAGCGTTGGCATCAAGTGGAGACCACCCCTCTCAGGGAGGAGAAACAGGTGCCCATTGCCCCTCTGCACCTATCCTTGGAGGACAGAAGTGAGCGGCTCTCCACACATGAGCTCACCTCTCTGCTAGAGAAGGAG
Peptide sequence:
MTSGIRRNWIQTIMKHVLPASAPDVTSSLPEGKNKSTSFETCSRSTEKQEAEPGEPDPEQKKSRARERRREGRSKTFDWAEFRPIQQALAQERASAVGSSDSGDPGCLEAEPGELERERARRREERRKRFGMLDTIDGPGMEDTALRMDIDRSPGLLGTPDLKTQNVHVEIEQRWHQVETTPLREEKQVPIAPLHLSLEDRSERLSTHELTSLLEKEAADLDGEINLSTCYDVTEYPVQRNYGFQIHTKEGEFTLSAMTSGIRRNWIQTIMKHVLPASAPDVTSSLPEGKNKSTSFETCSRSTEKQEAEPGEPDPEQKKSRARERRREGRSKTFDWAEFRPIQQALAQERASAVGSSDSGDPGCLEAEPGELERERARRREERRKRFGMLDTIDGPGMEDTALRMDIDRSPGLLGTPDLKTQNVHVEIEQRWHQVETTPLREEKQVPIAPLHLSLEDRSERLSTHELTSLLEKE
IRESfinder result
IRESfinder score
0.337943092
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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