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mmu_circMphosph9_833
Basic information of circular RNA
PanCircBase ID:
mmu_circMphosph9_833
mm39_Chr. Position ID:
chr5|124403379|124430470|-
Gene name:
Mphosph9
Transcript:
ENSMUST00000031344.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
16,15,14,13,12,11,10
Exon/intron offsets:
0,1722,18317,20152,21060,25529,27003
Genomic length:
27091
Exon/intron sizes in nt:
179,135,102,65,48,216,88
Flanking exon/introns:
chr5:124401098-124403379|chr5:124430470-124435617
Splice length:
833
Other database IDs:
circAtlas: mmu-Mphosph9_0029
Human Ortholog IDs:
Mature spliced Sequence:
GTGTGGCCAGCTGGATAGTGCATTGAATGAAGCTACTAGTCGAGTGCGCACACTTGAGAAGAACAACAACTTGCTAGAAATAGAAGTGAGTGACTTGAGAGAACGCTTCAATGCAGCCAGCAGTGCCTCCAAAGTTTTGCAGGAACGAATTGAAGAAATGAGGACAAGCAATAAGGAGAAAGATAACACCATCACTCGGCTAAAGTGTCGCCTGCAGGATCTGGAGGAAGCCTTCGAGAATGCTTACAAGCTCTCAGATGATAAGGAAGCGAGGCTGAGACAAGAAAACAAGATGTTTCAGGATCTCTTAGGAGAGTACGAATCCCTCGGCAAAGAACACGGGCGAGTAAAGGATACATTAAACACAACTGAGAACAAATTGCTTGATGCACACACTCAGATTTCTGACTTGAAAAGGACAATCTCAAAGCTTGAGGCTCAGGTGAAGCAGGCAGAGCACGAGAGTATGCTGAGTCTTCGCAATGGCGCTAAAGTTCCCGAGAGGCCGTCCCGGTCCAACTCCGTAGCCACCTCGGATGTCAGCAGGAGGAAGTGGCTGATTCCAGGAGCGGAGTACTCCATCTTTACTGGGCAGCCTCTGGACCCCAGGGACAGGAAGCTAGATAAGCAGCTGGAGGAAGCCCTTGTTCCTGGATACCATTCTCCTCCAGAAAAGGACTCTTCACTTGGAAGTTCACCAGCGAGCCTCCTGGTTAAAAAGAAGAGGGACACTCCGGACACACCACCGATCATCAAGGCTTTAAAGGAACTTGATGAAGAAAGAGTATTTAAAAGTTGGGGGACACAGACAGAGAAAGAGGATTCTTCAAGTA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CACCACCGATCATCAAGGCT
60.11
GCGCACTCGACTAGTAGCTT
59.9
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CACCACCGATCATCAAGGCT
60.11
GTGTGCGCACTCGACTAGTA
59.83
146
PCR template sequence:
CCGGACACACCACCGATCATCAAGGCTTTAAAGGAACTTGATGAAGAAAGAGTATTTAAAAGTTGGGGGACACAGACAGAGAAAGAGGATTCTTCAAGTAGTGTGGCCAGCTGGATAGTGCATTGAATGAAGCTACTAGTCGAGTGCGCACACTTGAGAAGAACAACAACTTGCTAGAAATAGAAGTGAGTGACTTGAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-434-5p(1), mmu-miR-541-5p(1), mmu-miR-3105-3p(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GATTCTTCAAGTAGTGTGGCCAGCTG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMPHOSPH9_833_ORF_1
1.0818
0.251064427
0.943856898
702
ORF sequence:
ATGAGGACAAGCAATAAGGAGAAAGATAACACCATCACTCGGCTAAAGTGTCGCCTGCAGGATCTGGAGGAAGCCTTCGAGAATGCTTACAAGCTCTCAGATGATAAGGAAGCGAGGCTGAGACAAGAAAACAAGATGTTTCAGGATCTCTTAGGAGAGTACGAATCCCTCGGCAAAGAACACGGGCGAGTAAAGGATACATTAAACACAACTGAGAACAAATTGCTTGATGCACACACTCAGATTTCTGACTTGAAAAGGACAATCTCAAAGCTTGAGGCTCAGGTGAAGCAGGCAGAGCACGAGAGTATGCTGAGTCTTCGCAATGGCGCTAAAGTTCCCGAGAGGCCGTCCCGGTCCAACTCCGTAGCCACCTCGGATGTCAGCAGGAGGAAGTGGCTGATTCCAGGAGCGGAGTACTCCATCTTTACTGGGCAGCCTCTGGACCCCAGGGACAGGAAGCTAGATAAGCAGCTGGAGGAAGCCCTTGTTCCTGGATACCATTCTCCTCCAGAAAAGGACTCTTCACTTGGAAGTTCACCAGCGAGCCTCCTGGTTAAAAAGAAGAGGGACACTCCGGACACACCACCGATCATCAAGGCTTTAAAGGAACTTGATGAAGAAAGAGTATTTAAAAGTTGGGGGACACAGACAGAGAAAGAGGATTCTTCAAGTAGTGTGGCCAGCTGGATAGTGCATTGA
Peptide sequence:
MRTSNKEKDNTITRLKCRLQDLEEAFENAYKLSDDKEARLRQENKMFQDLLGEYESLGKEHGRVKDTLNTTENKLLDAHTQISDLKRTISKLEAQVKQAEHESMLSLRNGAKVPERPSRSNSVATSDVSRRKWLIPGAEYSIFTGQPLDPRDRKLDKQLEEALVPGYHSPPEKDSSLGSSPASLLVKKKRDTPDTPPIIKALKELDEERVFKSWGTQTEKEDSSSSVASWIVH
IRESfinder result
IRESfinder score
0.501754515
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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