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mmu_circMorc3_644
Basic information of circular RNA
PanCircBase ID:
mmu_circMorc3_644
mm39_Chr. Position ID:
chr16|93641081|93646165|+
Gene name:
Morc3
Transcript:
ENSMUST00000044068.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
3,4,5,6
Exon/intron offsets:
0,946,3088,4936
Genomic length:
5084
Exon/intron sizes in nt:
1,33,21,41,49,148
Flanking exon/introns:
chr16:93638331-93641081|chr16:93646165-93649976
Splice length:
644
Other database IDs:
circAtlas: mmu-Morc3_0016
circBase: hsa_circ_0061615, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_96564
Human Ortholog IDs:
Mature spliced Sequence:
ATAATGCTTATGATCCTGATGTGAATGCTAAACAGATCTGGATCGATAAAACAGTGATTAGTGACCATATATGCTTGACATTCACTGATAATGGCAACGGGATGACAGCAGATAAGTTACATAAAATGCTAAGCTTTGGCTTCAGTGACAAAGTCACCATGAATGGTCATGTCCCAGTTGGATTGTACGGGAATGGCTTCAAGTCAGGTTCTATGCGACTGGGCAAAGATGCAATGGTTTTTACCAAAAATGGAGAAACCATGAGTGTGGGCTTCTTGTCTCAGACCTACCTGGAAGTCATAAAGGCGGAGCACGTTGTTGTCCCGATCGTGACGTTCAACAAACACCGTCAGATGATTAATTTAACAGAATCAAAAGCAAGCCTAGCAGCAATTCTGGAACATTCTCTGTTTTCTACGGAACAGAAATTACTGGCAGAACTCAATGCTATCATGGGTAAGAAGGGGACAAGAATCATCATTTGGAATCTCAGAAGTTACAAAAATGCAACAGAATTTGATTTTGAAAAGGATAAATACGACATCAGAATTCCTGAGGATTTAGATGAGACTGCAGGGAGGAAAGGGTACAAGAAACAAGAAAGAATGGACCAAATTGCCCCTGAGAGTGACTATTCCTTAAGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACTGCAGGGAGGAAAGGGTA
60.18
CCGTTGCCATTATCAGTGAATGT
59.62
175
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAGACTGCAGGGAGGAAAGG
59.75
CCGTTGCCATTATCAGTGAATGT
59.62
178
PCR template sequence:
CAGAATTCCTGAGGATTTAGATGAGACTGCAGGGAGGAAAGGGTACAAGAAACAAGAAAGAATGGACCAAATTGCCCCTGAGAGTGACTATTCCTTAAGGATAATGCTTATGATCCTGATGTGAATGCTAAACAGATCTGGATCGATAAAACAGTGATTAGTGACCATATATGCTTGACATTCACTGATAATGGCAACGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-136-5p(1), mmu-miR-541-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-452-3p(1), mmu-miR-188-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CTATTCCTTAAGGATAATGCTTATGA
siRNA sets
ATAAGCATTATCCTTAAGG, TAAGCATTATCCTTAAGGA, AAGCATTATCCTTAAGGAA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMORC3_644_ORF_1
1.1006
0.16425722
0.822991603
567
ORF sequence:
ATGACAGCAGATAAGTTACATAAAATGCTAAGCTTTGGCTTCAGTGACAAAGTCACCATGAATGGTCATGTCCCAGTTGGATTGTACGGGAATGGCTTCAAGTCAGGTTCTATGCGACTGGGCAAAGATGCAATGGTTTTTACCAAAAATGGAGAAACCATGAGTGTGGGCTTCTTGTCTCAGACCTACCTGGAAGTCATAAAGGCGGAGCACGTTGTTGTCCCGATCGTGACGTTCAACAAACACCGTCAGATGATTAATTTAACAGAATCAAAAGCAAGCCTAGCAGCAATTCTGGAACATTCTCTGTTTTCTACGGAACAGAAATTACTGGCAGAACTCAATGCTATCATGGGTAAGAAGGGGACAAGAATCATCATTTGGAATCTCAGAAGTTACAAAAATGCAACAGAATTTGATTTTGAAAAGGATAAATACGACATCAGAATTCCTGAGGATTTAGATGAGACTGCAGGGAGGAAAGGGTACAAGAAACAAGAAAGAATGGACCAAATTGCCCCTGAGAGTGACTATTCCTTAAGGATAATGCTTATGATCCTGATGTGA
Peptide sequence:
MTADKLHKMLSFGFSDKVTMNGHVPVGLYGNGFKSGSMRLGKDAMVFTKNGETMSVGFLSQTYLEVIKAEHVVVPIVTFNKHRQMINLTESKASLAAILEHSLFSTEQKLLAELNAIMGKKGTRIIIWNLRSYKNATEFDFEKDKYDIRIPEDLDETAGRKGYKKQERMDQIAPESDYSLRIMLMILM
IRESfinder result
IRESfinder score
0.577746707
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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