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mmu_circMorc2a_1005
Basic information of circular RNA
PanCircBase ID:
mmu_circMorc2a_1005
mm39_Chr. Position ID:
chr11|3608671|3628621|+
Gene name:
Morc2a
Transcript:
ENSMUST00000096441.5
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
11
Exon/intron indexes:
2,3,4,5,6,7,8,9,10,11,12
Exon/intron offsets:
0,3157,10099,10657,13594,17156,17432,17970,18731,19555,19864
Genomic length:
19950
Exon/intron sizes in nt:
54,35,69,91,109,160,112,126,80,83,86
Flanking exon/introns:
chr11:3600447-3608671|chr11:3628621-3629655
Splice length:
1005
Other database IDs:
circBase: hsa_circ_0062865, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_90438
Human Ortholog IDs:
Mature spliced Sequence:
AACCACTCATGAATTCTTATTTGGTGCTCTTGCTGAACTGGTTGATAATGCAAGAGATGCTGATGCTACCAGAATAGACATTTATGCTGAGAGGCGGGAGGACCTTCGAGGAGGATTTATGCTTTGCTTTTTGGATGATGGAGCAGGAATGGATCCAAGTGATGCTGCTAGTGTGATCCAGTTTGGGAAGTCAGCCAAAAGAACTCCTGAGTCCACTCAAATTGGGCAGTATGGCAATGGATTAAAATCGGGCTCAATGCGGATTGGGAAGGATTTTATCCTTTTCACTAAGAAGGAAGATACCATGACCTGCCTCTTCTTGTCTCGCACCTTTCATGAGGAAGAAGGCATTGATGAAGTAATTGTCCCATTGCCCACCTGGAATGCTCGAACACGGGAACCTATCACAGACAATGTGGAGAAGTTTGCCATTGAGACAGAGCTTGTCTACAAATACTCTCCCTTCCACACTGAGGAGCAAGTGATGAACCAGTTCATGAAGATTCCTGGGAATAGTGGAACACTGGTGATCATCTTTAATCTCAAGCTCATGGATAATGGAGAGCCGGAACTAGACATAATCTCAAATCCAAAAGATATCCAGATGGCAGAAACCTCGCCAGAGGGCACGAAGCCAGAGCGACGCTCCTTCCGAGCCTATGCTGCTGTACTCTATATTGATCCCCGGATGAGGATCTTCATCCATGGACACAAGGTGCAGACCAAGAGACTCTCCTGCTGCCTATATAAGCCCAGGATGTACAAGTATACATCAAGTCGTTTCAAGACTCGGGCAGAACAGGAAGTGAAGAAAGCAGAGCATGTAGCACGGATTGCTGAAGAGAAGGCCCGGGAGGCAGAGAGCAAAGCTCGTACTTTAGAAGTACGCATGGGTGGAGACCTGACACGGGACTCCAGGGTGATGTTGCGGCAGGTCCAGAATACAGCCATCACCCTTCGTAGAGAAGCTGATGTCAAGAAAAGGATCAAGGATGCCAAGCAGCG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCAGGTCCAGAATACAGCCA
59.75
CTCCCGCCTCTCAGCATAAA
59.53
175
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTGCGGCAGGTCCAGAATAC
60.39
CTCCCGCCTCTCAGCATAAA
59.53
180
PCR template sequence:
CACGGGACTCCAGGGTGATGTTGCGGCAGGTCCAGAATACAGCCATCACCCTTCGTAGAGAAGCTGATGTCAAGAAAAGGATCAAGGATGCCAAGCAGCGAACCACTCATGAATTCTTATTTGGTGCTCTTGCTGAACTGGTTGATAATGCAAGAGATGCTGATGCTACCAGAATAGACATTTATGCTGAGAGGCGGGAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
12
Interacting miRNAs (#binding sites)
mmu-miR-199a-5p(1), mmu-miR-103-3p(1), mmu-miR-221-3p(1), mmu-miR-15b-5p(1), mmu-miR-29b-3p(1), mmu-miR-29a-3p(1), mmu-miR-335-5p(1), mmu-miR-29c-3p(1), mmu-miR-362-5p(1), mmu-miR-107-3p(1), mmu-miR-669d-5p(1), mmu-miR-697(1)
siRNAs for circular RNA silencing
Junction sequence
ATGCCAAGCAGCGAACCACTCATGAA
siRNA sets
TTCATGAGTGGTTCGCTGC, AGTGGTTCGCTGCTTGGCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMORC2A_1005_ORF_1
1.0783
0.33990623
0.999992061
1890
ORF sequence:
ATGCTTTGCTTTTTGGATGATGGAGCAGGAATGGATCCAAGTGATGCTGCTAGTGTGATCCAGTTTGGGAAGTCAGCCAAAAGAACTCCTGAGTCCACTCAAATTGGGCAGTATGGCAATGGATTAAAATCGGGCTCAATGCGGATTGGGAAGGATTTTATCCTTTTCACTAAGAAGGAAGATACCATGACCTGCCTCTTCTTGTCTCGCACCTTTCATGAGGAAGAAGGCATTGATGAAGTAATTGTCCCATTGCCCACCTGGAATGCTCGAACACGGGAACCTATCACAGACAATGTGGAGAAGTTTGCCATTGAGACAGAGCTTGTCTACAAATACTCTCCCTTCCACACTGAGGAGCAAGTGATGAACCAGTTCATGAAGATTCCTGGGAATAGTGGAACACTGGTGATCATCTTTAATCTCAAGCTCATGGATAATGGAGAGCCGGAACTAGACATAATCTCAAATCCAAAAGATATCCAGATGGCAGAAACCTCGCCAGAGGGCACGAAGCCAGAGCGACGCTCCTTCCGAGCCTATGCTGCTGTACTCTATATTGATCCCCGGATGAGGATCTTCATCCATGGACACAAGGTGCAGACCAAGAGACTCTCCTGCTGCCTATATAAGCCCAGGATGTACAAGTATACATCAAGTCGTTTCAAGACTCGGGCAGAACAGGAAGTGAAGAAAGCAGAGCATGTAGCACGGATTGCTGAAGAGAAGGCCCGGGAGGCAGAGAGCAAAGCTCGTACTTTAGAAGTACGCATGGGTGGAGACCTGACACGGGACTCCAGGGTGATGTTGCGGCAGGTCCAGAATACAGCCATCACCCTTCGTAGAGAAGCTGATGTCAAGAAAAGGATCAAGGATGCCAAGCAGCGAACCACTCATGAATTCTTATTTGGTGCTCTTGCTGAACTGGTTGATAATGCAAGAGATGCTGATGCTACCAGAATAGACATTTATGCTGAGAGGCGGGAGGACCTTCGAGGAGGATTTATGCTTTGCTTTTTGGATGATGGAGCAGGAATGGATCCAAGTGATGCTGCTAGTGTGATCCAGTTTGGGAAGTCAGCCAAAAGAACTCCTGAGTCCACTCAAATTGGGCAGTATGGCAATGGATTAAAATCGGGCTCAATGCGGATTGGGAAGGATTTTATCCTTTTCACTAAGAAGGAAGATACCATGACCTGCCTCTTCTTGTCTCGCACCTTTCATGAGGAAGAAGGCATTGATGAAGTAATTGTCCCATTGCCCACCTGGAATGCTCGAACACGGGAACCTATCACAGACAATGTGGAGAAGTTTGCCATTGAGACAGAGCTTGTCTACAAATACTCTCCCTTCCACACTGAGGAGCAAGTGATGAACCAGTTCATGAAGATTCCTGGGAATAGTGGAACACTGGTGATCATCTTTAATCTCAAGCTCATGGATAATGGAGAGCCGGAACTAGACATAATCTCAAATCCAAAAGATATCCAGATGGCAGAAACCTCGCCAGAGGGCACGAAGCCAGAGCGACGCTCCTTCCGAGCCTATGCTGCTGTACTCTATATTGATCCCCGGATGAGGATCTTCATCCATGGACACAAGGTGCAGACCAAGAGACTCTCCTGCTGCCTATATAAGCCCAGGATGTACAAGTATACATCAAGTCGTTTCAAGACTCGGGCAGAACAGGAAGTGAAGAAAGCAGAGCATGTAGCACGGATTGCTGAAGAGAAGGCCCGGGAGGCAGAGAGCAAAGCTCGTACTTTAGAAGTACGCATGGGTGGAGACCTGACACGGGACTCCAGGGTGATGTTGCGGCAGGTCCAGAATACAGCCATCACCCTTCGTAGAGAAGCTGATGTCAAGAAAAGGATCAAGGATGCCAAGCAG
Peptide sequence:
MLCFLDDGAGMDPSDAASVIQFGKSAKRTPESTQIGQYGNGLKSGSMRIGKDFILFTKKEDTMTCLFLSRTFHEEEGIDEVIVPLPTWNARTREPITDNVEKFAIETELVYKYSPFHTEEQVMNQFMKIPGNSGTLVIIFNLKLMDNGEPELDIISNPKDIQMAETSPEGTKPERRSFRAYAAVLYIDPRMRIFIHGHKVQTKRLSCCLYKPRMYKYTSSRFKTRAEQEVKKAEHVARIAEEKAREAESKARTLEVRMGGDLTRDSRVMLRQVQNTAITLRREADVKKRIKDAKQRTTHEFLFGALAELVDNARDADATRIDIYAERREDLRGGFMLCFLDDGAGMDPSDAASVIQFGKSAKRTPESTQIGQYGNGLKSGSMRIGKDFILFTKKEDTMTCLFLSRTFHEEEGIDEVIVPLPTWNARTREPITDNVEKFAIETELVYKYSPFHTEEQVMNQFMKIPGNSGTLVIIFNLKLMDNGEPELDIISNPKDIQMAETSPEGTKPERRSFRAYAAVLYIDPRMRIFIHGHKVQTKRLSCCLYKPRMYKYTSSRFKTRAEQEVKKAEHVARIAEEKAREAESKARTLEVRMGGDLTRDSRVMLRQVQNTAITLRREADVKKRIKDAKQ
IRESfinder result
IRESfinder score
0.425599166
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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