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mmu_circMorc1_1015
Basic information of circular RNA
PanCircBase ID:
mmu_circMorc1_1015
mm39_Chr. Position ID:
chr16|48300190|48386079|+
Gene name:
Morc1
Transcript:
ENSMUST00000023330.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
9,10,11,12,13,14,15,16,17
Exon/intron offsets:
0,18919,20266,22477,30369,34758,78541,81707,85750
Genomic length:
85889
Exon/intron sizes in nt:
1,26,80,71,65,14,41,55,00,00,00,000
Flanking exon/introns:
chr16:48281148-48300190|chr16:48386079-48402779
Splice length:
1015
Other database IDs:
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
AATTCCAGAGAGACGGTCATTCAGAGCCTACACAGCTGTTCTGTATTTTGAACCCCGGATGAAAATATTTATTCAGGCCAAAAGAGTTCAAACAAAGCATCTGTGTTATTCCCTCTACAAACCCAGAAAATACCAATATACTACATCTTCTTTCAAAGGGAAGTTTAAAACTGAAGTTCAAAAGGCAGAAGAAGCAGTAAAGAGGGCTGAACTCCTGTTTAAAGAGGTGCAAGCCAAAGTAAACCAGCCGGACAGAATTGCTTTGTCTTCTACCCAGGATGCATTACAGAAAGCTCTGCAAGACGTGGACACAAAGCATAAAAGTCTTCGCCAGAAACAGAGGGCCCTAAGGAAAGCAAGAACTCTCTCTCTGTTCTTTGGAGTGAACACAGAAGACCAACACCAAGCTGGAATGTTCATTTACAGTAATAACCGATTGATCAAAATGTACGAGAAGGTTGGTCCCCAGCTGAAAATGAAGTCATTACTTGGTGCAGGTATAATTGGAATTGTGAACATACCTTTGGAGACCATGGAACCATCCCATAATAAACAAGAATTCCTCAATGTCCAAGAATACAATCATCTACTAAAAGTCATGGGACAGTACTTGATCCAGTACTGTAAGGACATTGGGATCAGTAATAGAAACCTAACACTGTTTTGGGACGAATTTAAATATCAGCATAGCAAAGACACAGACAGCTCTTTGGAATCTCTCCAATGGCGAAGAAGACAAGCCATGGGTATCCCATTCATCCTACAATGCGATCTTTGTCTCAAATGGAGAGTCCTGCCTTCCTCTTCCAATTACCAGGAAAAAGGATTACCTGACCTATGGATTTGTGCCAGTAATCCCAACAACCTGGAAAACAGCTGTAACCAGATAGAGCGCCTGCCTTCTATCCCACTGGGCACCGTGAACAGAAGACCACCATCAAAAGATGAGAGAGAGAGGCAACTTCAAGAGTCAGTCCAGAGATATCAGGACAAGCTGGTGGAAGCGCAGCCGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACCGTGAACAGAAGACCACC
59.89
GAACAGCTGTGTAGGCTCTGA
59.73
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCGTGAACAGAAGACCACCA
59.89
AGAACAGCTGTGTAGGCTCTG
59.73
140
PCR template sequence:
CACCGTGAACAGAAGACCACCATCAAAAGATGAGAGAGAGAGGCAACTTCAAGAGTCAGTCCAGAGATATCAGGACAAGCTGGTGGAAGCGCAGCCGCAGAATTCCAGAGAGACGGTCATTCAGAGCCTACACAGCTGTTCTGTATTTTGAACCCCGGATGAAAATATTTATTCAGGCCAAAAGAGTTCAAACAAAGCAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-135a-5p(1), mmu-miR-101a-3p(1), mmu-miR-101b-3p(1), mmu-miR-7a-5p(1), mmu-miR-136-5p(1), mmu-miR-431-5p(1), mmu-miR-29b-1-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AGCGCAGCCGCAGAATTCCAGAGAGA
siRNA sets
TCTCTCTGGAATTCTGCGG, CTCTCTGGAATTCTGCGGC, CTGGAATTCTGCGGCTGCG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMORC1_1015_ORF_1
0.6303
0.086315203
0.98323226
1086
ORF sequence:
ATGAGAGAGAGAGGCAACTTCAAGAGTCAGTCCAGAGATATCAGGACAAGCTGGTGGAAGCGCAGCCGCAGAATTCCAGAGAGACGGTCATTCAGAGCCTACACAGCTGTTCTGTATTTTGAACCCCGGATGAAAATATTTATTCAGGCCAAAAGAGTTCAAACAAAGCATCTGTGTTATTCCCTCTACAAACCCAGAAAATACCAATATACTACATCTTCTTTCAAAGGGAAGTTTAAAACTGAAGTTCAAAAGGCAGAAGAAGCAGTAAAGAGGGCTGAACTCCTGTTTAAAGAGGTGCAAGCCAAAGTAAACCAGCCGGACAGAATTGCTTTGTCTTCTACCCAGGATGCATTACAGAAAGCTCTGCAAGACGTGGACACAAAGCATAAAAGTCTTCGCCAGAAACAGAGGGCCCTAAGGAAAGCAAGAACTCTCTCTCTGTTCTTTGGAGTGAACACAGAAGACCAACACCAAGCTGGAATGTTCATTTACAGTAATAACCGATTGATCAAAATGTACGAGAAGGTTGGTCCCCAGCTGAAAATGAAGTCATTACTTGGTGCAGGTATAATTGGAATTGTGAACATACCTTTGGAGACCATGGAACCATCCCATAATAAACAAGAATTCCTCAATGTCCAAGAATACAATCATCTACTAAAAGTCATGGGACAGTACTTGATCCAGTACTGTAAGGACATTGGGATCAGTAATAGAAACCTAACACTGTTTTGGGACGAATTTAAATATCAGCATAGCAAAGACACAGACAGCTCTTTGGAATCTCTCCAATGGCGAAGAAGACAAGCCATGGGTATCCCATTCATCCTACAATGCGATCTTTGTCTCAAATGGAGAGTCCTGCCTTCCTCTTCCAATTACCAGGAAAAAGGATTACCTGACCTATGGATTTGTGCCAGTAATCCCAACAACCTGGAAAACAGCTGTAACCAGATAGAGCGCCTGCCTTCTATCCCACTGGGCACCGTGAACAGAAGACCACCATCAAAAGATGAGAGAGAGAGGCAACTTCAAGAGTCAGTCCAGAGATATCAGGACAAGCTGGTGGAAGCGCAGCCGCAG
Peptide sequence:
MRERGNFKSQSRDIRTSWWKRSRRIPERRSFRAYTAVLYFEPRMKIFIQAKRVQTKHLCYSLYKPRKYQYTTSSFKGKFKTEVQKAEEAVKRAELLFKEVQAKVNQPDRIALSSTQDALQKALQDVDTKHKSLRQKQRALRKARTLSLFFGVNTEDQHQAGMFIYSNNRLIKMYEKVGPQLKMKSLLGAGIIGIVNIPLETMEPSHNKQEFLNVQEYNHLLKVMGQYLIQYCKDIGISNRNLTLFWDEFKYQHSKDTDSSLESLQWRRRQAMGIPFILQCDLCLKWRVLPSSSNYQEKGLPDLWICASNPNNLENSCNQIERLPSIPLGTVNRRPPSKDERERQLQESVQRYQDKLVEAQPQ
IRESfinder result
IRESfinder score
0.706361259
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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