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mmu_circMlxip_262
Basic information of circular RNA
PanCircBase ID:
mmu_circMlxip_262
mm39_Chr. Position ID:
chr5|123581092|123582028|+
Gene name:
Mlxip
Transcript:
ENSMUST00000111596.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
6,7
Exon/intron offsets:
0,846
Genomic length:
936
Exon/intron sizes in nt:
172,90
Flanking exon/introns:
chr5:123580832-123581092|chr5:123582028-123582507
Splice length:
262
Other database IDs:
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_161339
Human Ortholog IDs:
Mature spliced Sequence:
GACGATGACATGCTGTATTGGCATAAACATGGGGATGGCTGGAAAACCCCAGTCCCCATGGAGGAGGACTCACTGCTGGACACAGACATGCTCATGTCAGAATTCAGCGACACTCTCTTCTCCACACTCTCTTCACACCAACCCGTGGCCTGGCCCAACCCACGAGAAATAGCACATCTGGGAAACGCAGACATGATCCAGCCAGGCCTGATCCCTTTGCAACCCAACCTGGACTTCATGGACACCTTTGAGCCTTTCCAGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTGCAACCCAACCTGGACTT
60.03
TGAGCATGTCTGTGTCCAGC
60.32
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTTGCAACCCAACCTGGACT
60.03
GAGCATGTCTGTGTCCAGCA
60.32
140
PCR template sequence:
CGAGAAATAGCACATCTGGGAAACGCAGACATGATCCAGCCAGGCCTGATCCCTTTGCAACCCAACCTGGACTTCATGGACACCTTTGAGCCTTTCCAGGGACGATGACATGCTGTATTGGCATAAACATGGGGATGGCTGGAAAACCCCAGTCCCCATGGAGGAGGACTCACTGCTGGACACAGACATGCTCATGTCAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GAGCCTTTCCAGGGACGATGACATGC
siRNA sets
GTCATCGTCCCTGGAAAGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMLXIP_262_ORF_1
1.0328
0.28401428
0.427989236
261
ORF sequence:
ATGCTGTATTGGCATAAACATGGGGATGGCTGGAAAACCCCAGTCCCCATGGAGGAGGACTCACTGCTGGACACAGACATGCTCATGTCAGAATTCAGCGACACTCTCTTCTCCACACTCTCTTCACACCAACCCGTGGCCTGGCCCAACCCACGAGAAATAGCACATCTGGGAAACGCAGACATGATCCAGCCAGGCCTGATCCCTTTGCAACCCAACCTGGACTTCATGGACACCTTTGAGCCTTTCCAGGGACGATGA
Peptide sequence:
MLYWHKHGDGWKTPVPMEEDSLLDTDMLMSEFSDTLFSTLSSHQPVAWPNPREIAHLGNADMIQPGLIPLQPNLDFMDTFEPFQGR
IRESfinder result
IRESfinder score
0.438621656
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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