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mmu_circMllt10_308
Basic information of circular RNA
PanCircBase ID:
mmu_circMllt10_308
mm39_Chr. Position ID:
chr2|18114592|18128623|+
Gene name:
Mllt10
Transcript:
ENSMUST00000028076.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
5,6,7
Exon/intron offsets:
0,12463,13937
Genomic length:
14031
Exon/intron sizes in nt:
110,104,94
Flanking exon/introns:
chr2:18106323-18114592|chr2:18128623-18130944
Splice length:
308
Other database IDs:
circAtlas: mmu-Mllt10_0054
CIRCpedia: HSA_CIRCpedia_348102
Human Ortholog IDs:
Mature spliced Sequence:
GTTGGGCTCATGTGGTTTGTGCCCTCTATATTCCAGAGGTACAATTTGCCAATGTTTCTACAATGGAGCCAATCGTTTTACAGTCTGTCCCACATGATCGTTATAATAAGACTTGCTATATATGTGATGAACAAGGACGAGAAAGCAAAGCAGCCACTGGTGCTTGCATGACATGTAATAAACATGGATGTCGACAGGCTTTCCATGTAACATGTGCTCAGTTTGCCGGGCTGCTTTGTGAAGAAGAAGGGAATGGCGCAGACAATGTTCAATACTGTGGCTACTGTAAATACCACTTTAGTAAGCTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AAGAAGAAGGGAATGGCGCA
59.96
CGATCATGTGGGACAGACTGT
59.79
168
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAAGAAGGGAATGGCGCAGA
60.39
CGATCATGTGGGACAGACTGT
59.79
166
PCR template sequence:
AACATGTGCTCAGTTTGCCGGGCTGCTTTGTGAAGAAGAAGGGAATGGCGCAGACAATGTTCAATACTGTGGCTACTGTAAATACCACTTTAGTAAGCTGGTTGGGCTCATGTGGTTTGTGCCCTCTATATTCCAGAGGTACAATTTGCCAATGTTTCTACAATGGAGCCAATCGTTTTACAGTCTGTCCCACATGATCG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-300-3p(1), mmu-miR-350-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CTTTAGTAAGCTGGTTGGGCTCATGT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMLLT10_308_ORF_1
0.5301
-0.031922973
0.198919344
417
ORF sequence:
ATGGAGCCAATCGTTTTACAGTCTGTCCCACATGATCGTTATAATAAGACTTGCTATATATGTGATGAACAAGGACGAGAAAGCAAAGCAGCCACTGGTGCTTGCATGACATGTAATAAACATGGATGTCGACAGGCTTTCCATGTAACATGTGCTCAGTTTGCCGGGCTGCTTTGTGAAGAAGAAGGGAATGGCGCAGACAATGTTCAATACTGTGGCTACTGTAAATACCACTTTAGTAAGCTGGTTGGGCTCATGTGGTTTGTGCCCTCTATATTCCAGAGGTACAATTTGCCAATGTTTCTACAATGGAGCCAATCGTTTTACAGTCTGTCCCACATGATCGTTATAATAAGACTTGCTATATATGTGATGAACAAGGACGAGAAAGCAAAGCAGCCACTGGTGCTTGCATGA
Peptide sequence:
MEPIVLQSVPHDRYNKTCYICDEQGRESKAATGACMTCNKHGCRQAFHVTCAQFAGLLCEEEGNGADNVQYCGYCKYHFSKLVGLMWFVPSIFQRYNLPMFLQWSQSFYSLSHMIVIIRLAIYVMNKDEKAKQPLVLA
IRESfinder result
IRESfinder score
0.465888697
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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