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mmu_circMllt10_295
Basic information of circular RNA
PanCircBase ID:
mmu_circMllt10_295
mm39_Chr. Position ID:
chr2|18075935|18087536|+
Gene name:
Mllt10
Transcript:
ENSMUST00000153279.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
3,4
Exon/intron offsets:
0,11386
Genomic length:
11601
Exon/intron sizes in nt:
80,215
Flanking exon/introns:
chr2:18070005-18075935|chr2:18087536-18106268
Splice length:
295
Other database IDs:
CIRCpedia: MMU_CIRCpedia_12300, circAtlas: mmu-Mllt10_0142
Human Ortholog IDs:
Mature spliced Sequence:
CTTGCTATGGCATTGTTCAAGTACCTACTGGACCGTGGTTTTGTAGGAAATGTGAATCTCAGGAGAGAGCAGCCAGAGTGACTCTCTACTACATGAGGCTGGAACCATGACTGCTGGCAGCTCTTGGCTTTCATCTTCCCAGCCCCTTCTACCAGGAAAAGGGGCTCTTTTGAAGAATCCTGAGAAGGACTCTGATTGACCTGTCTTGGATCATGTACCCATCATCCCTCTGGCTGGAGTGGGGAGGGCTCTATGAATTGCAACCCTCACTAGAACTACATAGTTGGAGTCAGGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATCATCCCTCTGGCTGGAGT
60.03
TGGCTGCTCTCTCCTGAGAT
60.03
150
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATCATCCCTCTGGCTGGAGT
60.03
GTCACTCTGGCTGCTCTCTC
59.83
157
PCR template sequence:
TTGACCTGTCTTGGATCATGTACCCATCATCCCTCTGGCTGGAGTGGGGAGGGCTCTATGAATTGCAACCCTCACTAGAACTACATAGTTGGAGTCAGGGCTTGCTATGGCATTGTTCAAGTACCTACTGGACCGTGGTTTTGTAGGAAATGTGAATCTCAGGAGAGAGCAGCCAGAGTGACTCTCTACTACATGAGGCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-22-3p(1), mmu-miR-452-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GTTGGAGTCAGGGCTTGCTATGGCAT
siRNA sets
ATGCCATAGCAAGCCCTGA, TAGCAAGCCCTGACTCCAA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMLLT10_295_ORF_1
1.0384
-0.230699619
0.034285168
129
ORF sequence:
ATGTACCCATCATCCCTCTGGCTGGAGTGGGGAGGGCTCTATGAATTGCAACCCTCACTAGAACTACATAGTTGGAGTCAGGGCTTGCTATGGCATTGTTCAAGTACCTACTGGACCGTGGTTTTGTAG
Peptide sequence:
MYPSSLWLEWGGLYELQPSLELHSWSQGLLWHCSSTYWTVVL
IRESfinder result
IRESfinder score
0.71239803
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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