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mmu_circMios_1917
Basic information of circular RNA
PanCircBase ID:
mmu_circMios_1917
mm39_Chr. Position ID:
chr6|8211579|8224724|+
Gene name:
Mios
Transcript:
ENSMUST00000040017.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
2,3,4,5,6
Exon/intron offsets:
0,3186,4792,10881,12975
Genomic length:
13145
Exon/intron sizes in nt:
59,1334,99,255,170
Flanking exon/introns:
chr6:8209411-8211579|chr6:8224724-8226843
Splice length:
1917
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GGACATAGCAAGAATCTCAAGACATGTTTCAGAAGCTCCCTCTATTTGTGAGAATCTAGTGAATGGACCTGACTGGACTTTTTAAGCCCATCAATAAACATGAGCGGTACCAAGCCCGATATTTTGTGGGCACCACACCAAGTTGATAGATTTGTTGTATGTGACTCAGAATTGAGCCTTTATCATGTGGAATCGGCTGTGAATTCAGAACTCAAAGCTGGATCGTTACGTTTATCTGAAGACTCTGCAGCGACATTGCTGTCAATAAACTCAGATACACCCTATATGAAATGTGTTGCGTGGTATCTCAATTATGATCCTGAATGTCTCCTAGCAGTTGGACAAGCAAATGGTCGAGTTGTACTTACAAGTCTTGGTCAAGATCATAACTCTAAGTTCAAAGATTTGATAGGAAAAGAATTTGTTCCAAAGCATGCTCGCCAATGCAATACCCTTGCATGGAATCCACTGGATAGTAACTGGCTTGCTGCTGGTCTCGACAAACACAGAGCTGACTTTTCAGTTCTAATATGGGATATCTGCAGCAAATATACTCCTGATATTGTTCCCATGGAAAAAGTGAGACTTTCAGCAGGTGAAGCTGAAACAACATTACTAGTGACAAAACCGCTGTATGAGTTAGGGCAGAATGACGCTTCTCTATCTCTGTGTTGGCTTCCACGAGACCAGAAACTTCTTCTTGCTGGCATGCATCGTAATCTAGCAATATTTGATCTCCGGAACACAAGCCAGAAGATGTTTGTAAATACAAAAGCTGTTCAAGGAGTGACAGTAGACCCTTACTTCCACGATCGTGTTGCTTCCTTCTATGAAGGTCAGGTTGCAATATGGGATCTTAGAAAATTTGAGAAGCCAGTTTTGACTTTGACTGAACAACCAAAGCCCTTGACAAAGGTCGCATGGTGTCCAACCAGGACAGGCCTGCTTGCTACTTTAACAAGGGATAGTAATATTATTAGGCTGTATGATATGCAGCATACACCCACTCCCATCGGGGATGAAACCGAACCCACAATAATTGAAAGAAGTGTGCAGCCTTGTGACAATTACATTGCTTCCTTTGCATGGCATCCAACGAGTCAAAATCGAATGATAGTTGTGACTCCCAACCGAACAATGTCTGACTTCACTGTATTTGAAAGGATATCTCTTGCCTGGAGCCCAATTACATCTTTAATGTGGGCTTGTGGTCGCCATTTGTATGAGTGTGCAGAAGAAGAAAGTGATAACTCCTTAGAAAAAGATATAGCAACAAAAATGCGCCTTCGGGCTTTATCAAGGTATGGACTTGATACAGAACAGGTTTGGAGAAACCACATTTTAGCTGGCAATGAAGACCCACAGCTCAAGTCACTCTGGTATACTCTGCACTTTATGAAGCAATATACAGAAGATAATGATCAAAAATCTCCAGGCAACAAAGGATCATTGGTTTATGCAGGAATTAAATCAATAGTAAAATCATCTTTGGGAATGGTAGAAAGCAGCAGACATAATTGGAGTGGCTTGGATAAACAAACTGATATTCAGAACTTAAATGAAGAAAGGATTTTAGCATTACAGCTTTGTGGGTGGATAAAGAAAGGAACGGATGTAGATGTAGGGCCATTTTTGAACTCCCTTGTGCAAGAAGGAGAATGGGAAAGAGCTGCTGCTGTGGCTTTGTTCAACTTGGATATTCGGCGAGCAATCCAAATCCTGAATGAAGGGGCGTCTTCAGAAAAAGGAGATCTGAATCTCAATGTGGTGGCAATGGCTTTATCGGGGTATACGGACGAGAAGAACTCGCTGTGGCGGGAGATGTGCAGCACCCTGCGCCTGCAGCTCAACAACCCCTATCTGTGTGTCATGTTTGCGTTCCTGACGAGTGAAGCGGGGGCATATGATGGAGTGTTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTGCAGCTCAACAACCCCTA
59.96
GTCCAGTCAGGTCCATTCACT
59.37
156
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTGCAGCTCAACAACCCCTA
59.96
AGTCCAGTCAGGTCCATTCAC
59.37
157
PCR template sequence:
GGAGATGTGCAGCACCCTGCGCCTGCAGCTCAACAACCCCTATCTGTGTGTCATGTTTGCGTTCCTGACGAGTGAAGCGGGGGCATATGATGGAGTGTTGGGACATAGCAAGAATCTCAAGACATGTTTCAGAAGCTCCCTCTATTTGTGAGAATCTAGTGAATGGACCTGACTGGACTTTTTAAGCCCATCAATAAACA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
16
Interacting miRNAs (#binding sites)
mmu-miR-137-3p(2), mmu-miR-322-5p(2), mmu-miR-9-5p(1), mmu-miR-155-5p(1), mmu-miR-16-5p(1), mmu-miR-375-3p(1), mmu-miR-205-5p(1), mmu-miR-106b-5p(1), mmu-miR-33-5p(1), mmu-miR-328-3p(1), mmu-miR-181b-5p(1), mmu-miR-297a-5p(1), mmu-miR-300-3p(1), mmu-miR-544-5p(1), mmu-miR-181d-5p(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TGATGGAGTGTTGGGACATAGCAAGA
siRNA sets
TCTTGCTATGTCCCAACAC, CTTGCTATGTCCCAACACT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMIOS_1917_ORF_1
0.8364
-0.000384449
0.999939236
1869
ORF sequence:
ATGAGCGGTACCAAGCCCGATATTTTGTGGGCACCACACCAAGTTGATAGATTTGTTGTATGTGACTCAGAATTGAGCCTTTATCATGTGGAATCGGCTGTGAATTCAGAACTCAAAGCTGGATCGTTACGTTTATCTGAAGACTCTGCAGCGACATTGCTGTCAATAAACTCAGATACACCCTATATGAAATGTGTTGCGTGGTATCTCAATTATGATCCTGAATGTCTCCTAGCAGTTGGACAAGCAAATGGTCGAGTTGTACTTACAAGTCTTGGTCAAGATCATAACTCTAAGTTCAAAGATTTGATAGGAAAAGAATTTGTTCCAAAGCATGCTCGCCAATGCAATACCCTTGCATGGAATCCACTGGATAGTAACTGGCTTGCTGCTGGTCTCGACAAACACAGAGCTGACTTTTCAGTTCTAATATGGGATATCTGCAGCAAATATACTCCTGATATTGTTCCCATGGAAAAAGTGAGACTTTCAGCAGGTGAAGCTGAAACAACATTACTAGTGACAAAACCGCTGTATGAGTTAGGGCAGAATGACGCTTCTCTATCTCTGTGTTGGCTTCCACGAGACCAGAAACTTCTTCTTGCTGGCATGCATCGTAATCTAGCAATATTTGATCTCCGGAACACAAGCCAGAAGATGTTTGTAAATACAAAAGCTGTTCAAGGAGTGACAGTAGACCCTTACTTCCACGATCGTGTTGCTTCCTTCTATGAAGGTCAGGTTGCAATATGGGATCTTAGAAAATTTGAGAAGCCAGTTTTGACTTTGACTGAACAACCAAAGCCCTTGACAAAGGTCGCATGGTGTCCAACCAGGACAGGCCTGCTTGCTACTTTAACAAGGGATAGTAATATTATTAGGCTGTATGATATGCAGCATACACCCACTCCCATCGGGGATGAAACCGAACCCACAATAATTGAAAGAAGTGTGCAGCCTTGTGACAATTACATTGCTTCCTTTGCATGGCATCCAACGAGTCAAAATCGAATGATAGTTGTGACTCCCAACCGAACAATGTCTGACTTCACTGTATTTGAAAGGATATCTCTTGCCTGGAGCCCAATTACATCTTTAATGTGGGCTTGTGGTCGCCATTTGTATGAGTGTGCAGAAGAAGAAAGTGATAACTCCTTAGAAAAAGATATAGCAACAAAAATGCGCCTTCGGGCTTTATCAAGGTATGGACTTGATACAGAACAGGTTTGGAGAAACCACATTTTAGCTGGCAATGAAGACCCACAGCTCAAGTCACTCTGGTATACTCTGCACTTTATGAAGCAATATACAGAAGATAATGATCAAAAATCTCCAGGCAACAAAGGATCATTGGTTTATGCAGGAATTAAATCAATAGTAAAATCATCTTTGGGAATGGTAGAAAGCAGCAGACATAATTGGAGTGGCTTGGATAAACAAACTGATATTCAGAACTTAAATGAAGAAAGGATTTTAGCATTACAGCTTTGTGGGTGGATAAAGAAAGGAACGGATGTAGATGTAGGGCCATTTTTGAACTCCCTTGTGCAAGAAGGAGAATGGGAAAGAGCTGCTGCTGTGGCTTTGTTCAACTTGGATATTCGGCGAGCAATCCAAATCCTGAATGAAGGGGCGTCTTCAGAAAAAGGAGATCTGAATCTCAATGTGGTGGCAATGGCTTTATCGGGGTATACGGACGAGAAGAACTCGCTGTGGCGGGAGATGTGCAGCACCCTGCGCCTGCAGCTCAACAACCCCTATCTGTGTGTCATGTTTGCGTTCCTGACGAGTGAAGCGGGGGCATATGATGGAGTGTTGGGACATAGCAAGAATCTCAAGACATGTTTCAGAAGCTCCCTCTATTTGTGA
Peptide sequence:
MSGTKPDILWAPHQVDRFVVCDSELSLYHVESAVNSELKAGSLRLSEDSAATLLSINSDTPYMKCVAWYLNYDPECLLAVGQANGRVVLTSLGQDHNSKFKDLIGKEFVPKHARQCNTLAWNPLDSNWLAAGLDKHRADFSVLIWDICSKYTPDIVPMEKVRLSAGEAETTLLVTKPLYELGQNDASLSLCWLPRDQKLLLAGMHRNLAIFDLRNTSQKMFVNTKAVQGVTVDPYFHDRVASFYEGQVAIWDLRKFEKPVLTLTEQPKPLTKVAWCPTRTGLLATLTRDSNIIRLYDMQHTPTPIGDETEPTIIERSVQPCDNYIASFAWHPTSQNRMIVVTPNRTMSDFTVFERISLAWSPITSLMWACGRHLYECAEEESDNSLEKDIATKMRLRALSRYGLDTEQVWRNHILAGNEDPQLKSLWYTLHFMKQYTEDNDQKSPGNKGSLVYAGIKSIVKSSLGMVESSRHNWSGLDKQTDIQNLNEERILALQLCGWIKKGTDVDVGPFLNSLVQEGEWERAAAVALFNLDIRRAIQILNEGASSEKGDLNLNVVAMALSGYTDEKNSLWREMCSTLRLQLNNPYLCVMFAFLTSEAGAYDGVLGHSKNLKTCFRSSLYL
IRESfinder result
IRESfinder score
0.659063112
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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