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mmu_circMical2_1863
Basic information of circular RNA
PanCircBase ID:
mmu_circMical2_1863
mm39_Chr. Position ID:
chr7|111964169|111981444|+
Gene name:
Mical2
Transcript:
ENSMUST00000215412.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
4,5,6,7,8
Exon/intron offsets:
0,3317,11052,15320,15993
Genomic length:
17275
Exon/intron sizes in nt:
181,179,75,146,1282
Flanking exon/introns:
chr7:111953161-111964169|chr7:111981444-111990472
Splice length:
1863
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATGAGCCCATCTCTCCCAAGAAGCCAAAGAGTGTCCCTGAGCCCAACCCCAGAGACGTGGAGGCTGAAGCCACCTCTCCTCGGCCGTCTGAATGGACTTCAGTGAGGATCAGCCCTGGGGAGGATACAGTCAGGCAGGACGTGCTGGCTGTACGTGTCCTAGTCACCAGTGAGGACAGCAGCTCTGATACAGAATCCGACTACGGCAGCATCACAGCCCCCTGTGCAGGGGCCTATGAAGAAAGACCTCAGCTTCCAGAATCCCCACCTCTATCAAAGCCCCTAACCCGGCACATCTCCCTACGGGAGAACCTGACCCAACCAGTCTCTCTGCTACACGAAGAGCCACAAGCTCTGCCTGCTCTGCAGCGGGCCCATAGCCTCCAGTCTCCAACTCCAAGCAAATACCAGAACTGGAGAAGAAGATTCCAGTCCAATTCAACACCCATGAACCAAAGAGCACCATCGCCTCCAAAGGAGCCGCCTCCTCCTCCATCTTTGTCTTCTTCATCTTCCCTACCATCTTCATTTTCTTCAGCCAGTGTACCTGGGCACACTGCAGACGATTCCTCCTCTCCCCAGGTGCCAGCTTACAACCTCCACTCCCCACAGATCTCCAGAGGCGATGTCAGCCCCACTCCAATCTACCTCAGACGTGCCAGGGCTCAAGGGATTGTGAAGGAAATTCCCCTCTACCTGCCGCATAGCCCCATGCTAGAGAGCACAGAAGACTGCCTGGTCGAGCCCGGTAGAGAAAGCCTCAGAAGTCCTGAGGAGATCTCCTCTTCAGAGGGCTGCCAGGAAGCCCGGGCTCTTGGAAACACCAGAAGCATACAGCACCCCATTTTAGGGAAGGACCAGTATTTGCCCAATCAAAACCTAGCTCTTGGTGCTGCAGGGAACCCTGGAGACCCGAGGGAGGAATCCAGGATGGGACAACCAGGTGGACCAGAGCTGTCCAAAGAAAGGAAGTTGGGCTTGAAGAAATTAGTCCTCACTGAGGAGCAGAAGAACAAGTTGCTAGACTGGAGTGACTGCACCCAGGAGCACAAGACTGGGGAGCAGCTCTCTCAGGAAAGTGCTGAGAACATCAGAGGTGGTAGCCTGAAGCCAACCTGCTCTTCTACCCTCTCTCAGGCGGTGAAAGAGAAGCTGCTATCCCAGAAAAAGGCCCTGGGGGGGATGCGTACCCCAGCTGTCAAAGCACCTCAGGAGCGAGAGGTGCCCCCACCCAAGTCCCCCCTGAAGCTCATAGCAAATGCCATCCTTAGGTCTCTGCTCCACAACTCCGAAGCTGGAAAGAAAACCAGCCCTAAGCCAGAGTCCAAAACTTTGCCTAGGGGCCAGCCACATGCTCGTTCGTTCAGCCTCCGGAAACTGGGTTCCAGTAAAGATGGGGACCAGCAGTCTCCAGGGAGACACATGGCCAAGAAGGCTTCAGCATTCTTCTCTCTGGCTTCTCCAACATCTAAGGTTGCCCAAGCCTCAGACCTTAGCCTTCCCAACTCCATCCTCCGCAGCCGGAGTTTACCGAGTCGTCCATCCAAGATGTTCTTTTCAACCACACCTCACAGCAAGGTTGAAGATGTCCCCACACTCCTGGAGAAAGTGAGTTTGCAGGATGCCACTCACAGTCCAAAGACAGGAGCCTCACATATTTCTTCCCTTGGCCTCAAAGATAAATCCTTTGAGAGTTTTCTCCAGGAATGCAAACAAAGAAAGGACATTGGGGACTTCTTTAATAGCCCCAAGGAGGAGGGGCCACCAGGGAACAGAGTCCCATCCTTGGAGAAGTTGGTACAGCCTGTTGGCAGCACCTCCATGGGACAGGTGGCACATCCTTCTTCCACAGGGCAAGATGCAC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGAACAGAGTCCCATCCTTGG
60.07
CTTCAGCCTCCACGTCTCTG
60.11
165
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACAGAGTCCCATCCTTGGAGA
59.92
CTTCAGCCTCCACGTCTCTG
60.11
162
PCR template sequence:
CCAGGGAACAGAGTCCCATCCTTGGAGAAGTTGGTACAGCCTGTTGGCAGCACCTCCATGGGACAGGTGGCACATCCTTCTTCCACAGGGCAAGATGCACATGAGCCCATCTCTCCCAAGAAGCCAAAGAGTGTCCCTGAGCCCAACCCCAGAGACGTGGAGGCTGAAGCCACCTCTCCTCGGCCGTCTGAATGGACTTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-let-7b-5p(1), mmu-miR-324-5p(1), mmu-miR-125b-1-3p(1), mmu-miR-342-5p(1), mmu-miR-690(1), mmu-miR-207(1), mmu-miR-5101(1), mmu-miR-3087-3p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
GGGCAAGATGCACATGAGCCCATCTC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMICAL2_1863_ORF_1
0.7213
0.207286833
0.999999999
3279
ORF sequence:
ATGAACCAAAGAGCACCATCGCCTCCAAAGGAGCCGCCTCCTCCTCCATCTTTGTCTTCTTCATCTTCCCTACCATCTTCATTTTCTTCAGCCAGTGTACCTGGGCACACTGCAGACGATTCCTCCTCTCCCCAGGTGCCAGCTTACAACCTCCACTCCCCACAGATCTCCAGAGGCGATGTCAGCCCCACTCCAATCTACCTCAGACGTGCCAGGGCTCAAGGGATTGTGAAGGAAATTCCCCTCTACCTGCCGCATAGCCCCATGCTAGAGAGCACAGAAGACTGCCTGGTCGAGCCCGGTAGAGAAAGCCTCAGAAGTCCTGAGGAGATCTCCTCTTCAGAGGGCTGCCAGGAAGCCCGGGCTCTTGGAAACACCAGAAGCATACAGCACCCCATTTTAGGGAAGGACCAGTATTTGCCCAATCAAAACCTAGCTCTTGGTGCTGCAGGGAACCCTGGAGACCCGAGGGAGGAATCCAGGATGGGACAACCAGGTGGACCAGAGCTGTCCAAAGAAAGGAAGTTGGGCTTGAAGAAATTAGTCCTCACTGAGGAGCAGAAGAACAAGTTGCTAGACTGGAGTGACTGCACCCAGGAGCACAAGACTGGGGAGCAGCTCTCTCAGGAAAGTGCTGAGAACATCAGAGGTGGTAGCCTGAAGCCAACCTGCTCTTCTACCCTCTCTCAGGCGGTGAAAGAGAAGCTGCTATCCCAGAAAAAGGCCCTGGGGGGGATGCGTACCCCAGCTGTCAAAGCACCTCAGGAGCGAGAGGTGCCCCCACCCAAGTCCCCCCTGAAGCTCATAGCAAATGCCATCCTTAGGTCTCTGCTCCACAACTCCGAAGCTGGAAAGAAAACCAGCCCTAAGCCAGAGTCCAAAACTTTGCCTAGGGGCCAGCCACATGCTCGTTCGTTCAGCCTCCGGAAACTGGGTTCCAGTAAAGATGGGGACCAGCAGTCTCCAGGGAGACACATGGCCAAGAAGGCTTCAGCATTCTTCTCTCTGGCTTCTCCAACATCTAAGGTTGCCCAAGCCTCAGACCTTAGCCTTCCCAACTCCATCCTCCGCAGCCGGAGTTTACCGAGTCGTCCATCCAAGATGTTCTTTTCAACCACACCTCACAGCAAGGTTGAAGATGTCCCCACACTCCTGGAGAAAGTGAGTTTGCAGGATGCCACTCACAGTCCAAAGACAGGAGCCTCACATATTTCTTCCCTTGGCCTCAAAGATAAATCCTTTGAGAGTTTTCTCCAGGAATGCAAACAAAGAAAGGACATTGGGGACTTCTTTAATAGCCCCAAGGAGGAGGGGCCACCAGGGAACAGAGTCCCATCCTTGGAGAAGTTGGTACAGCCTGTTGGCAGCACCTCCATGGGACAGGTGGCACATCCTTCTTCCACAGGGCAAGATGCACATGAGCCCATCTCTCCCAAGAAGCCAAAGAGTGTCCCTGAGCCCAACCCCAGAGACGTGGAGGCTGAAGCCACCTCTCCTCGGCCGTCTGAATGGACTTCAGTGAGGATCAGCCCTGGGGAGGATACAGTCAGGCAGGACGTGCTGGCTGTACGTGTCCTAGTCACCAGTGAGGACAGCAGCTCTGATACAGAATCCGACTACGGCAGCATCACAGCCCCCTGTGCAGGGGCCTATGAAGAAAGACCTCAGCTTCCAGAATCCCCACCTCTATCAAAGCCCCTAACCCGGCACATCTCCCTACGGGAGAACCTGACCCAACCAGTCTCTCTGCTACACGAAGAGCCACAAGCTCTGCCTGCTCTGCAGCGGGCCCATAGCCTCCAGTCTCCAACTCCAAGCAAATACCAGAACTGGAGAAGAAGATTCCAGTCCAATTCAACACCCATGAACCAAAGAGCACCATCGCCTCCAAAGGAGCCGCCTCCTCCTCCATCTTTGTCTTCTTCATCTTCCCTACCATCTTCATTTTCTTCAGCCAGTGTACCTGGGCACACTGCAGACGATTCCTCCTCTCCCCAGGTGCCAGCTTACAACCTCCACTCCCCACAGATCTCCAGAGGCGATGTCAGCCCCACTCCAATCTACCTCAGACGTGCCAGGGCTCAAGGGATTGTGAAGGAAATTCCCCTCTACCTGCCGCATAGCCCCATGCTAGAGAGCACAGAAGACTGCCTGGTCGAGCCCGGTAGAGAAAGCCTCAGAAGTCCTGAGGAGATCTCCTCTTCAGAGGGCTGCCAGGAAGCCCGGGCTCTTGGAAACACCAGAAGCATACAGCACCCCATTTTAGGGAAGGACCAGTATTTGCCCAATCAAAACCTAGCTCTTGGTGCTGCAGGGAACCCTGGAGACCCGAGGGAGGAATCCAGGATGGGACAACCAGGTGGACCAGAGCTGTCCAAAGAAAGGAAGTTGGGCTTGAAGAAATTAGTCCTCACTGAGGAGCAGAAGAACAAGTTGCTAGACTGGAGTGACTGCACCCAGGAGCACAAGACTGGGGAGCAGCTCTCTCAGGAAAGTGCTGAGAACATCAGAGGTGGTAGCCTGAAGCCAACCTGCTCTTCTACCCTCTCTCAGGCGGTGAAAGAGAAGCTGCTATCCCAGAAAAAGGCCCTGGGGGGGATGCGTACCCCAGCTGTCAAAGCACCTCAGGAGCGAGAGGTGCCCCCACCCAAGTCCCCCCTGAAGCTCATAGCAAATGCCATCCTTAGGTCTCTGCTCCACAACTCCGAAGCTGGAAAGAAAACCAGCCCTAAGCCAGAGTCCAAAACTTTGCCTAGGGGCCAGCCACATGCTCGTTCGTTCAGCCTCCGGAAACTGGGTTCCAGTAAAGATGGGGACCAGCAGTCTCCAGGGAGACACATGGCCAAGAAGGCTTCAGCATTCTTCTCTCTGGCTTCTCCAACATCTAAGGTTGCCCAAGCCTCAGACCTTAGCCTTCCCAACTCCATCCTCCGCAGCCGGAGTTTACCGAGTCGTCCATCCAAGATGTTCTTTTCAACCACACCTCACAGCAAGGTTGAAGATGTCCCCACACTCCTGGAGAAAGTGAGTTTGCAGGATGCCACTCACAGTCCAAAGACAGGAGCCTCACATATTTCTTCCCTTGGCCTCAAAGATAAATCCTTTGAGAGTTTTCTCCAGGAATGCAAACAAAGAAAGGACATTGGGGACTTCTTTAATAGCCCCAAGGAGGAGGGGCCACCAGGGAACAGAGTCCCATCCTTGGAGAAGTTGGTACAGCCTGTTGGCAGCACCTCCATGGGACAGGTGGCACATCCTTCTTCCACAGGGCAAGATGCA
Peptide sequence:
MNQRAPSPPKEPPPPPSLSSSSSLPSSFSSASVPGHTADDSSSPQVPAYNLHSPQISRGDVSPTPIYLRRARAQGIVKEIPLYLPHSPMLESTEDCLVEPGRESLRSPEEISSSEGCQEARALGNTRSIQHPILGKDQYLPNQNLALGAAGNPGDPREESRMGQPGGPELSKERKLGLKKLVLTEEQKNKLLDWSDCTQEHKTGEQLSQESAENIRGGSLKPTCSSTLSQAVKEKLLSQKKALGGMRTPAVKAPQEREVPPPKSPLKLIANAILRSLLHNSEAGKKTSPKPESKTLPRGQPHARSFSLRKLGSSKDGDQQSPGRHMAKKASAFFSLASPTSKVAQASDLSLPNSILRSRSLPSRPSKMFFSTTPHSKVEDVPTLLEKVSLQDATHSPKTGASHISSLGLKDKSFESFLQECKQRKDIGDFFNSPKEEGPPGNRVPSLEKLVQPVGSTSMGQVAHPSSTGQDAHEPISPKKPKSVPEPNPRDVEAEATSPRPSEWTSVRISPGEDTVRQDVLAVRVLVTSEDSSSDTESDYGSITAPCAGAYEERPQLPESPPLSKPLTRHISLRENLTQPVSLLHEEPQALPALQRAHSLQSPTPSKYQNWRRRFQSNSTPMNQRAPSPPKEPPPPPSLSSSSSLPSSFSSASVPGHTADDSSSPQVPAYNLHSPQISRGDVSPTPIYLRRARAQGIVKEIPLYLPHSPMLESTEDCLVEPGRESLRSPEEISSSEGCQEARALGNTRSIQHPILGKDQYLPNQNLALGAAGNPGDPREESRMGQPGGPELSKERKLGLKKLVLTEEQKNKLLDWSDCTQEHKTGEQLSQESAENIRGGSLKPTCSSTLSQAVKEKLLSQKKALGGMRTPAVKAPQEREVPPPKSPLKLIANAILRSLLHNSEAGKKTSPKPESKTLPRGQPHARSFSLRKLGSSKDGDQQSPGRHMAKKASAFFSLASPTSKVAQASDLSLPNSILRSRSLPSRPSKMFFSTTPHSKVEDVPTLLEKVSLQDATHSPKTGASHISSLGLKDKSFESFLQECKQRKDIGDFFNSPKEEGPPGNRVPSLEKLVQPVGSTSMGQVAHPSSTGQDA
IRESfinder result
IRESfinder score
0.575874115
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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