top of page
mmu_circMib1_836
Basic information of circular RNA
PanCircBase ID:
mmu_circMib1_836
mm39_Chr. Position ID:
chr18|10792894|10802339|+
Gene name:
Mib1
Transcript:
ENSMUST00000052838.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
13,14,15,16,17,18
Exon/intron offsets:
0,1583,2795,5457,7161,9366
Genomic length:
9445
Exon/intron sizes in nt:
133,87,162,182,193,79
Flanking exon/introns:
chr18:10778300-10792894|chr18:10802339-10804686
Splice length:
836
Other database IDs:
circAtlas: mmu-Mib1_0029
CIRCpedia: HSA_CIRCpedia_88278
Human Ortholog IDs:
Mature spliced Sequence:
TGCAATGCGTGTTTTACTCTCAAAATTACCAAGACCATGGATTGTGGATGAGAAGAAAGATGATGGTTATACTGCCTTGCACCTGGCTGCCCTGAATAATCATGTGGAAGTGGCTGAGCTGTTGGTGCATCAGGGAAATGCAAACCTGGATATCCAGAATGTGAACCAGCAAACAGCCCTACACCTTGCTGTTGAACGGCAACACACCCAGATTGTTAGGCTTTTGGTCCGTGCAGGTGCTAAGCTAGACATTCAGGATAAGGATGGAGATACTCCTCTGCATGAAGCTTTGAGACATCATACTTTGTCTCAACTACGTCAGCTACAAGACATGCAAGATGTGGGCAAGGTAGACGCTGCCTGGGAACCTTCAAAAAACACATTAATAATGGGACTTGGTACCCAGGGGGCTGAGAAGAAGAGTGCAGCATCCATTGCGTGTTTCTTGGCAGCCAATGGTGCTGATCTCAGCATTCGAAATAAGAAGGGTCAGTCACCACTGGATCTCTGTCCTGACCCAAGTCTCTGCAAAGCTTTGGCAAAATGTCATAAGGAAAAAGTCAGTGGCCAAGTGGGTTCTCGGAGTCCTTCTATGATTAGCAACGATTCTGAAACTTTAGAGGAATGTATGGTGTGCTCAGACATGAAGAGAGATACTCTTTTTGGTCCGTGTGGACACATTGCAACCTGTTCTTTGTGTTCCCCTCGTGTCAAGAAATGCCTCATTTGTAAAGAGCAGGTTCAGTCCAGGACAAAGATTGAAGAATGTGTGGTGTGCTCCGACAAGAAAGCAGCTGTTCTCTTTCAACCTTGTGGGCACATGTGTGCTTGTGAGA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCCGACAAGAAAGCAGCTGT
59.89
TTATTCAGGGCAGCCAGGTG
60.03
157
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGTGCTCCGACAAGAAAGCA
60.18
TTATTCAGGGCAGCCAGGTG
60.03
162
PCR template sequence:
CAGGTTCAGTCCAGGACAAAGATTGAAGAATGTGTGGTGTGCTCCGACAAGAAAGCAGCTGTTCTCTTTCAACCTTGTGGGCACATGTGTGCTTGTGAGATGCAATGCGTGTTTTACTCTCAAAATTACCAAGACCATGGATTGTGGATGAGAAGAAAGATGATGGTTATACTGCCTTGCACCTGGCTGCCCTGAATAAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-29b-3p(1), mmu-miR-29c-3p(1), mmu-miR-298-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGTGCTTGTGAGATGCAATGCGTGTT
siRNA sets
AACACGCATTGCATCTCAC, ACACGCATTGCATCTCACA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMIB1_836_ORF_1
1.0734
0.068483802
0.975481435
927
ORF sequence:
ATGCGTGTTTTACTCTCAAAATTACCAAGACCATGGATTGTGGATGAGAAGAAAGATGATGGTTATACTGCCTTGCACCTGGCTGCCCTGAATAATCATGTGGAAGTGGCTGAGCTGTTGGTGCATCAGGGAAATGCAAACCTGGATATCCAGAATGTGAACCAGCAAACAGCCCTACACCTTGCTGTTGAACGGCAACACACCCAGATTGTTAGGCTTTTGGTCCGTGCAGGTGCTAAGCTAGACATTCAGGATAAGGATGGAGATACTCCTCTGCATGAAGCTTTGAGACATCATACTTTGTCTCAACTACGTCAGCTACAAGACATGCAAGATGTGGGCAAGGTAGACGCTGCCTGGGAACCTTCAAAAAACACATTAATAATGGGACTTGGTACCCAGGGGGCTGAGAAGAAGAGTGCAGCATCCATTGCGTGTTTCTTGGCAGCCAATGGTGCTGATCTCAGCATTCGAAATAAGAAGGGTCAGTCACCACTGGATCTCTGTCCTGACCCAAGTCTCTGCAAAGCTTTGGCAAAATGTCATAAGGAAAAAGTCAGTGGCCAAGTGGGTTCTCGGAGTCCTTCTATGATTAGCAACGATTCTGAAACTTTAGAGGAATGTATGGTGTGCTCAGACATGAAGAGAGATACTCTTTTTGGTCCGTGTGGACACATTGCAACCTGTTCTTTGTGTTCCCCTCGTGTCAAGAAATGCCTCATTTGTAAAGAGCAGGTTCAGTCCAGGACAAAGATTGAAGAATGTGTGGTGTGCTCCGACAAGAAAGCAGCTGTTCTCTTTCAACCTTGTGGGCACATGTGTGCTTGTGAGATGCAATGCGTGTTTTACTCTCAAAATTACCAAGACCATGGATTGTGGATGAGAAGAAAGATGATGGTTATACTGCCTTGCACCTGGCTGCCCTGA
Peptide sequence:
MRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSLCKALAKCHKEKVSGQVGSRSPSMISNDSETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACEMQCVFYSQNYQDHGLWMRRKMMVILPCTWLP
IRESfinder result
IRESfinder score
0.589978114
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page