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mmu_circMib1_235
Basic information of circular RNA
PanCircBase ID:
mmu_circMib1_235
mm39_Chr. Position ID:
chr18|10743268|10747460|+
Gene name:
Mib1
Transcript:
ENSMUST00000052838.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
3,4
Exon/intron offsets:
0,4087
Genomic length:
4192
Exon/intron sizes in nt:
1,30,105
Flanking exon/introns:
chr18:10741154-10743268|chr18:10747460-10749322
Splice length:
235
Other database IDs:
circBase: hsa_circ_0108041, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_113479
Human Ortholog IDs:
Mature spliced Sequence:
GGTTCTGTTAGAGTCTCGTAGAAAATCTAAGAAGATTACAGCCAGAGGAATCTTTGCTGGTGCCAGAGTGGTGCGAGGAGTGGACTGGCAGTGGGAAGATCAAGATGGAGGCAATGGGCGTAGAGGAAAGGTCACGGAGATCCAGGACTGGAGTGCATCCAGCCCACACAGTGCAGCCTATGTTCTCTGGGATAACGGTGCTAAGAACCTTTACAGGGTTGGCTTTGAGGGCATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCACACAGTGCAGCCTATGT
60.32
ACTCTGGCACCAGCAAAGAT
59.6
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCACACAGTGCAGCCTATGT
60.32
CACTCTGGCACCAGCAAAGA
60.54
142
PCR template sequence:
GGAGATCCAGGACTGGAGTGCATCCAGCCCACACAGTGCAGCCTATGTTCTCTGGGATAACGGTGCTAAGAACCTTTACAGGGTTGGCTTTGAGGGCATGGGTTCTGTTAGAGTCTCGTAGAAAATCTAAGAAGATTACAGCCAGAGGAATCTTTGCTGGTGCCAGAGTGGTGCGAGGAGTGGACTGGCAGTGGGAAGAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-10b-5p(1), mmu-miR-10a-5p(1), mmu-miR-96-5p(1), mmu-miR-183-5p(1), mmu-miR-3470b(1)
siRNAs for circular RNA silencing
Junction sequence
CTTTGAGGGCATGGGTTCTGTTAGAG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMIB1_235_ORF_1
0.629
0.117719807
0.276489896
366
ORF sequence:
ATGGAGGCAATGGGCGTAGAGGAAAGGTCACGGAGATCCAGGACTGGAGTGCATCCAGCCCACACAGTGCAGCCTATGTTCTCTGGGATAACGGTGCTAAGAACCTTTACAGGGTTGGCTTTGAGGGCATGGGTTCTGTTAGAGTCTCGTAGAAAATCTAAGAAGATTACAGCCAGAGGAATCTTTGCTGGTGCCAGAGTGGTGCGAGGAGTGGACTGGCAGTGGGAAGATCAAGATGGAGGCAATGGGCGTAGAGGAAAGGTCACGGAGATCCAGGACTGGAGTGCATCCAGCCCACACAGTGCAGCCTATGTTCTCTGGGATAACGGTGCTAAGAACCTTTACAGGGTTGGCTTTGAGGGCATG
Peptide sequence:
MEAMGVEERSRRSRTGVHPAHTVQPMFSGITVLRTFTGLALRAWVLLESRRKSKKITARGIFAGARVVRGVDWQWEDQDGGNGRRGKVTEIQDWSASSPHSAAYVLWDNGAKNLYRVGFEGM
IRESfinder result
IRESfinder score
0.469378526
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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