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mmu_circMib1_1815
Basic information of circular RNA
PanCircBase ID:
mmu_circMib1_1815
mm39_Chr. Position ID:
chr18|10763188|10812156|+
Gene name:
Mib1
Transcript:
ENSMUST00000124288.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
13
Exon/intron indexes:
2,3,4,5,6,7,8,9,10,11,12,13,14
Exon/intron offsets:
0,4935,12340,14960,29706,31289,32501,35163,36867,39072,41498,44845,48796
Genomic length:
48968
Exon/intron sizes in nt:
13,41,08,19,81,52,13,30,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr18:10760948-10763188|chr18:10812156-10817818
Splice length:
1815
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AAAGACTCTCCCAGCTTCTGAAGAAATTATTTGAAACCCAAGAATCTGGTGACCTCAATGAAGAGCTGGTTAAGGCTGCTGCCAATGGAGACGTGGCTAAAGTGGAAGATTTGCTGAAAAGACCAGATGTGGATGTAAACGGACAGTGTGCTGGCCACACAGCAATGCAAGCTGCTAGTCAGAACGGACATGTTGATATTTTGAAATTACTTTTGAAGCAAAATGTTGATGTTGAAGCAGAGGATAAAGATGGAGATCGAGCCGTTCACCATGCAGCCTTTGGAGATGAGGGTGCTGTCATAGAAGTCCTACACCGAGGCAGTGCTGACCTGAATGCTCGTAATAAGCGTAGACAGACGCCTCTTCATATTGCTGTCAATAAAGGTCATCTTCAAGTTGTGAAGACTTTGTTGGATTTTGGTTGTCATCCCAGTCTCCAGGATTCTGAAGGTGACACCCCTCTCCATGATGCCATAAGTAAGAAACGGGATGACATTCTGGCAGTTCTTCTGGAAGCTGGAGCAGATGTGACCATCACGAACAATAACGGGTTCAATGCCCTGCACCATGCTGCACTGAGAGGGAACCCCAGTGCAATGCGTGTTTTACTCTCAAAATTACCAAGACCATGGATTGTGGATGAGAAGAAAGATGATGGTTATACTGCCTTGCACCTGGCTGCCCTGAATAATCATGTGGAAGTGGCTGAGCTGTTGGTGCATCAGGGAAATGCAAACCTGGATATCCAGAATGTGAACCAGCAAACAGCCCTACACCTTGCTGTTGAACGGCAACACACCCAGATTGTTAGGCTTTTGGTCCGTGCAGGTGCTAAGCTAGACATTCAGGATAAGGATGGAGATACTCCTCTGCATGAAGCTTTGAGACATCATACTTTGTCTCAACTACGTCAGCTACAAGACATGCAAGATGTGGGCAAGGTAGACGCTGCCTGGGAACCTTCAAAAAACACATTAATAATGGGACTTGGTACCCAGGGGGCTGAGAAGAAGAGTGCAGCATCCATTGCGTGTTTCTTGGCAGCCAATGGTGCTGATCTCAGCATTCGAAATAAGAAGGGTCAGTCACCACTGGATCTCTGTCCTGACCCAAGTCTCTGCAAAGCTTTGGCAAAATGTCATAAGGAAAAAGTCAGTGGCCAAGTGGGTTCTCGGAGTCCTTCTATGATTAGCAACGATTCTGAAACTTTAGAGGAATGTATGGTGTGCTCAGACATGAAGAGAGATACTCTTTTTGGTCCGTGTGGACACATTGCAACCTGTTCTTTGTGTTCCCCTCGTGTCAAGAAATGCCTCATTTGTAAAGAGCAGGTTCAGTCCAGGACAAAGATTGAAGAATGTGTGGTGTGCTCCGACAAGAAAGCAGCTGTTCTCTTTCAACCTTGTGGGCACATGTGTGCTTGTGAGAACTGTGCCAGCCTGATGAAAAAGTGTGTGCAGTGTCGGGCAGTGGTTGAGCGGAGGGTGCCTTTCATCACGTGCTGTGGTGGCAAGAGCTCAGAAGACCCCTCTGATGAAATCTCAAGTGGGAATATCCCAGTGCTGCAGAAGGACAAAGACAACACCAATGTTAACGCAGATGTGCAGAAACTGCAGCAGCAGCTTCAGGACATTAAGGAGCAGACCATGTGCCCTGTGTGCTTGGATCGCCTGAAGAACATGATTTTCCTCTGTGGCCATGGAACATGCCAGCTCTGTGGAGACCGGATGAGTGAATGCCCAATTTGTCGCAAGGCAATTGAACGAAGGATTCTTTTGTACTGATTAAGAAACACAGAGTGTTTTGTTAGCTAAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGACCGGATGAGTGAATGCC
59.82
CAGCAGCCTTAACCAGCTCT
60.04
176
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTCGCAAGGCAATTGAACGA
59.76
CAGCAGCCTTAACCAGCTCT
60.04
150
PCR template sequence:
TGTGGAGACCGGATGAGTGAATGCCCAATTTGTCGCAAGGCAATTGAACGAAGGATTCTTTTGTACTGATTAAGAAACACAGAGTGTTTTGTTAGCTAAAAAAGACTCTCCCAGCTTCTGAAGAAATTATTTGAAACCCAAGAATCTGGTGACCTCAATGAAGAGCTGGTTAAGGCTGCTGCCAATGGAGACGTGGCTAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
12
Interacting miRNAs (#binding sites)
mmu-miR-298-5p(2), mmu-miR-324-3p(2), mmu-miR-375-3p(1), mmu-miR-103-3p(1), mmu-miR-92a-3p(1), mmu-miR-29b-3p(1), mmu-miR-29c-3p(1), mmu-miR-25-3p(1), mmu-miR-363-3p(1), mmu-miR-676-3p(1), mmu-miR-107-3p(1), mmu-miR-29b-2-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TTTGTTAGCTAAAAAAGACTCTCCCA
siRNA sets
TGGGAGAGTCTTTTTTAGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.501635348
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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