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mmu_circMia3_665
Basic information of circular RNA
PanCircBase ID:
mmu_circMia3_665
mm39_Chr. Position ID:
chr1|183112158|183116406|-
Gene name:
Mia3
Transcript:
ENSMUST00000194543.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
22,21,20,19,18,17,16,15
Exon/intron offsets:
0,172,329,1521,2805,3099,3278,4136
Genomic length:
4248
Exon/intron sizes in nt:
68,36,61,10,21,39,91,50,000
Flanking exon/introns:
chr1:183112047-183112158|chr1:183116406-183116484
Splice length:
665
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GCTTTGACTAATTGCATCACACAGCTGAATCGGTTAGAATGTGAACTTGAATCTGAGGATCCAGATAAAGGAGGAAATGAGTCAGATGATCTGGCCAATGGAGAAACGGGAGGTGACCGGAGCGAGAAGATAAGAAATAGAATTAAGCAGATGATGGACGTCTCTCGGACACAAACTGCAGTCTCAATAGTTGAAGAGGATCTAAAACTTTTACAGCTTAAGCTAAGAGCATCGATGTCCACTAAATGTAACCTGGAAGACCAAATCAAGAAATTAGAAGATGACCGCAGCTCGCTGCAGACTGCTAAAGCTGGGTTGGAAGACGAGTGCAAAACTCTGAGGCAGAAAGTGGAGATCCTGAATGAGCTGTACCAGCAGAAGGAGATGGCGCTGCAGAAGAAACTGAGTCAAGAAGAGTATGAGCGGCAAGACAGAGAGCAGAGGCTCACGGCGGCCGATGAGAAGGTGGTTCTGGCTGCAGAGGAAGTGAAAACGTACAAGCGGCGAATCGAAGAAATGGAAGAAGAATTACAGAAAACAGAACGTTCATTTAAAAACCAGATTGCTGCTCATGAGAAGAAAGCTCATGATAATTGGCTCAAAGCTCGTGCTGCAGAGAGAGCCATGGCAGAGGAGAAGAGAGAAGCTGCTAACTTAAGGCACAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AATTGGCTCAAAGCTCGTGC
59.76
TCCTCCTTTATCTGGATCCTCAGA
59.83
149
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AATTGGCTCAAAGCTCGTGC
59.76
CCTCCTTTATCTGGATCCTCAGA
58.51
148
PCR template sequence:
CTGCTCATGAGAAGAAAGCTCATGATAATTGGCTCAAAGCTCGTGCTGCAGAGAGAGCCATGGCAGAGGAGAAGAGAGAAGCTGCTAACTTAAGGCACAAGCTTTGACTAATTGCATCACACAGCTGAATCGGTTAGAATGTGAACTTGAATCTGAGGATCCAGATAAAGGAGGAAATGAGTCAGATGATCTGGCCAATG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-23b-3p(1), mmu-miR-15a-5p(1), mmu-miR-92a-3p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-23a-3p(1), mmu-miR-136-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
ACTTAAGGCACAAGCTTTGACTAATT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMIA3_665_ORF_1
1.1449
0.370471823
0.894403257
522
ORF sequence:
ATGATGGACGTCTCTCGGACACAAACTGCAGTCTCAATAGTTGAAGAGGATCTAAAACTTTTACAGCTTAAGCTAAGAGCATCGATGTCCACTAAATGTAACCTGGAAGACCAAATCAAGAAATTAGAAGATGACCGCAGCTCGCTGCAGACTGCTAAAGCTGGGTTGGAAGACGAGTGCAAAACTCTGAGGCAGAAAGTGGAGATCCTGAATGAGCTGTACCAGCAGAAGGAGATGGCGCTGCAGAAGAAACTGAGTCAAGAAGAGTATGAGCGGCAAGACAGAGAGCAGAGGCTCACGGCGGCCGATGAGAAGGTGGTTCTGGCTGCAGAGGAAGTGAAAACGTACAAGCGGCGAATCGAAGAAATGGAAGAAGAATTACAGAAAACAGAACGTTCATTTAAAAACCAGATTGCTGCTCATGAGAAGAAAGCTCATGATAATTGGCTCAAAGCTCGTGCTGCAGAGAGAGCCATGGCAGAGGAGAAGAGAGAAGCTGCTAACTTAAGGCACAAGCTTTGA
Peptide sequence:
MMDVSRTQTAVSIVEEDLKLLQLKLRASMSTKCNLEDQIKKLEDDRSSLQTAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQDREQRLTAADEKVVLAAEEVKTYKRRIEEMEEELQKTERSFKNQIAAHEKKAHDNWLKARAAERAMAEEKREAANLRHKL
IRESfinder result
IRESfinder score
0.257422703
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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