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mmu_circMia3_291
Basic information of circular RNA
PanCircBase ID:
mmu_circMia3_291
mm39_Chr. Position ID:
chr1|183115436|183116607|-
Gene name:
Mia3
Transcript:
ENSMUST00000194543.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
16,15,14
Exon/intron offsets:
0,858,1048
Genomic length:
1171
Exon/intron sizes in nt:
5,61,12,123
Flanking exon/introns:
chr1:183115348-183115436|chr1:183116607-183117373
Splice length:
291
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TTGCAGCAGCAGGTAGAAGAGTGGAGTAAATCGCATGCCGAGCTCACTGAACAGATCAAGTCATTTGAGAAGTCCCAGGAAGACTTGGAAATAGCTCTGACTCACAAAGACGACAATATTAGTGCTTTGACTAATTGCATCACACAGCTGAATCGGTTAGAATGTGAACTTGAATCTGAGGATCCAGATAAAGGAGGAAATGAGTCAGATGATCTGGCCAATGGAGAAACGGGAGGTGACCGGAGCGAGAAGATAAGAAATAGAATTAAGCAGATGATGGACGTCTCTCGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAGATGATCTGGCCAATGGAGA
59.89
ATCTGTTCAGTGAGCTCGGC
60.11
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCAGATGATCTGGCCAATGGAG
59.89
ATCTGTTCAGTGAGCTCGGC
60.11
143
PCR template sequence:
AGGAGGAAATGAGTCAGATGATCTGGCCAATGGAGAAACGGGAGGTGACCGGAGCGAGAAGATAAGAAATAGAATTAAGCAGATGATGGACGTCTCTCGGTTGCAGCAGCAGGTAGAAGAGTGGAGTAAATCGCATGCCGAGCTCACTGAACAGATCAAGTCATTTGAGAAGTCCCAGGAAGACTTGGAAATAGCTCTGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-23b-3p(1), mmu-miR-23a-3p(1), mmu-miR-136-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGACGTCTCTCGGTTGCAGCAGCAGG
siRNA sets
CTGCTGCAACCGAGAGACG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMIA3_291_ORF_1
1.0212
0.362378075
0.587535659
309
ORF sequence:
ATGATGGACGTCTCTCGGTTGCAGCAGCAGGTAGAAGAGTGGAGTAAATCGCATGCCGAGCTCACTGAACAGATCAAGTCATTTGAGAAGTCCCAGGAAGACTTGGAAATAGCTCTGACTCACAAAGACGACAATATTAGTGCTTTGACTAATTGCATCACACAGCTGAATCGGTTAGAATGTGAACTTGAATCTGAGGATCCAGATAAAGGAGGAAATGAGTCAGATGATCTGGCCAATGGAGAAACGGGAGGTGACCGGAGCGAGAAGATAAGAAATAGAATTAAGCAGATGATGGACGTCTCTCGG
Peptide sequence:
MMDVSRLQQQVEEWSKSHAELTEQIKSFEKSQEDLEIALTHKDDNISALTNCITQLNRLECELESEDPDKGGNESDDLANGETGGDRSEKIRNRIKQMMDVSR
IRESfinder result
IRESfinder score
0.460935255
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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