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mmu_circMga_1716
Basic information of circular RNA
PanCircBase ID:
mmu_circMga_1716
mm39_Chr. Position ID:
chr2|119751846|119763623|+
Gene name:
Mga
Transcript:
ENSMUST00000046717.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
6,7,8,9,10,11,12
Exon/intron offsets:
0,372,2062,5013,10345,11138,11707
Genomic length:
11777
Exon/intron sizes in nt:
132,105,659,343,224,183,70
Flanking exon/introns:
chr2:119750388-119751846|chr2:119763623-119765629
Splice length:
1716
Other database IDs:
CIRCpedia: MMU_CIRCpedia_4995, circAtlas: mmu-Mga_0013
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
TGGGCTTAAAACTGAATTCAGTGGATCCAACAGTGAGCATTGATCTTAAATACTTGGGAGTACAATTGCCTTTGGCTCCAGCCACCAGCTTTCCCCTATGGAATGTTACAGGTACCAACCCTGCCTCACCTGATGCTGGGTTTCCTTTTGTTTCTAGGACGGGGAAGACCAATGACTTTACCAAAATCAAGGGATGGAGAGGAAAATTTCAAAATGCTTCTGCATCTAGGAATGAAGGTGGAAATTCAGAGGCTTCACTGAAAAACCGTTCTGCTTTCTGTAGTGATAAGCTAGATGAGTACTTGGAAAATGAAGGCAAATTGATGGAAACAAACATAGGTTTCTCTTCAAATGCTCCCACATCTCCAGTAGTGTACCAGCTACCCACCAAGAGTACCAGCTATGTTCGAACTCTTGATAGTGTACTAAAGAAGCAATCTACCATTTCCCCTTCTACCTCTCACTCTGTGAAGCCTCAGTCTGTAACCACTGCCTCTCGAAAAACAAAGGCTCAGAACAAACAGACAACACTCAGTGGCCGAACTAAATCATCTTATAAGTCTATTTTACCATACCCTGTTTCACCAAAGCAGAAAAACTCTCATGTGAGCCAAGGAGATAAAATTACCAAGAATTCCTTGAGTTCGACCTCAGATAATCAGGTGACTAACTTGGTTGTGCCATCTGTAGATGAAAATGCATTTCCAAAGCAGATTAGTTTGCGGCAGGCCCAGCAACAGCACCTTCAGCAGCAAGGAACTCGCCCTCCAGGCTTGTCGAAATCTCAAGTAAAGCTTATGGACCTGGAAGACTGTGCACTCTGGGAAGGAAAACCAAGGACCTATATTACTGAAGAGCGAGCAGATGTCTCCTTGACAACTCTGCTTACTGCTCAGGCATCTCTCAAAACTAAACCTATCCACACAATCATAAGGAAACGAGCTCCTCCATGCAACAATGACTTCTGTCGCCTGGGTTGTGTGTGCTCTAGTCTAGCTTTGGAAAAGCGCCAACCTGCTCACTGCCGTCGACCAGACTGCATGTTTGGTTGCACTTGTTTGAAAAGAAAAGTTGTGTTGGTTAAAGGGGGATCTAAAACAAAGCATTTCCATAAGAAGGCTGCTAATCGAGATCCATTATTTTATGATACGCTGGGAGAGGAAGGAAGGGAAGGAGGAGGAGTCAGAGAAGATGAGGAACAATTGAAGGAGAAAAAGAAGAGAAAGAAGCTCGAATACACTGTATGTGAAGCAGAACCTGAGCAGCCGGTTCGACATTACCCACTGTGGGTGAAGGTAGAAGGTGAAGTAGATCCAGAGCCGGTTTATATTCCAACTCCTTCTGTCATTGAACCTATAAAACCATTGGTGTTGCCTCAGCCGGATTTATCTTCTACTACGAAGGGCAAACTAACCCCTGGAATTAAACCAGCACGAACATATACTCCCAAGCCCAATCCTGTGATACGAGAAGAGGACAAAGATCCAGTCTATTTGTACTTTGAAAGTATGATGACTTGTGCCCGAGTTCGAGTATATGAACGAAAGAAAGAGGAACAGAGACAACTGTCTCCACCCCTGTCTCCATCCTCATCATTTCAGCAGCAGAGTTCCTGTTACTCTAGTCCTGAGAACCGTGTTACAAAGGAACTTGATTCTGAACAGACCTTAAAGCAGCTCATTTGTGACTTGGAGGATGATTCTGATAAATCACAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCAGCTCATTTGTGACTTGGA
59.12
CATAGGGGAAAGCTGGTGGC
60.75
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGCAGCTCATTTGTGACTTGG
59.12
CATAGGGGAAAGCTGGTGGC
60.75
145
PCR template sequence:
TACTCTAGTCCTGAGAACCGTGTTACAAAGGAACTTGATTCTGAACAGACCTTAAAGCAGCTCATTTGTGACTTGGAGGATGATTCTGATAAATCACAAGTGGGCTTAAAACTGAATTCAGTGGATCCAACAGTGAGCATTGATCTTAAATACTTGGGAGTACAATTGCCTTTGGCTCCAGCCACCAGCTTTCCCCTATG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
28
Interacting miRNAs (#binding sites)
mmu-miR-34c-5p(2), mmu-miR-320-3p(2), mmu-let-7d-5p(2), mmu-miR-9-5p(1), mmu-miR-140-5p(1), mmu-let-7a-5p(1), mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-miR-34a-5p(1), mmu-miR-205-5p(1), mmu-let-7c-5p(1), mmu-miR-203-3p(1), mmu-miR-138-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-721(1), mmu-miR-194-5p(1), mmu-miR-34b-5p(1), mmu-miR-224-5p(1), mmu-miR-98-5p(1), mmu-miR-705(1), mmu-miR-1198-5p(1), mmu-miR-207(1), mmu-miR-5101(1), mmu-miR-1904(1), mmu-miR-338-3p(1), mmu-miR-7a-2-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GATAAATCACAAGTGGGCTTAAAACT
siRNA sets
TTTTAAGCCCACTTGTGAT, AGTTTTAAGCCCACTTGTG, TTTAAGCCCACTTGTGATT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMGA_1716_ORF_1
0.9071
0.09667095
0.999999995
3108
ORF sequence:
ATGGAAACAAACATAGGTTTCTCTTCAAATGCTCCCACATCTCCAGTAGTGTACCAGCTACCCACCAAGAGTACCAGCTATGTTCGAACTCTTGATAGTGTACTAAAGAAGCAATCTACCATTTCCCCTTCTACCTCTCACTCTGTGAAGCCTCAGTCTGTAACCACTGCCTCTCGAAAAACAAAGGCTCAGAACAAACAGACAACACTCAGTGGCCGAACTAAATCATCTTATAAGTCTATTTTACCATACCCTGTTTCACCAAAGCAGAAAAACTCTCATGTGAGCCAAGGAGATAAAATTACCAAGAATTCCTTGAGTTCGACCTCAGATAATCAGGTGACTAACTTGGTTGTGCCATCTGTAGATGAAAATGCATTTCCAAAGCAGATTAGTTTGCGGCAGGCCCAGCAACAGCACCTTCAGCAGCAAGGAACTCGCCCTCCAGGCTTGTCGAAATCTCAAGTAAAGCTTATGGACCTGGAAGACTGTGCACTCTGGGAAGGAAAACCAAGGACCTATATTACTGAAGAGCGAGCAGATGTCTCCTTGACAACTCTGCTTACTGCTCAGGCATCTCTCAAAACTAAACCTATCCACACAATCATAAGGAAACGAGCTCCTCCATGCAACAATGACTTCTGTCGCCTGGGTTGTGTGTGCTCTAGTCTAGCTTTGGAAAAGCGCCAACCTGCTCACTGCCGTCGACCAGACTGCATGTTTGGTTGCACTTGTTTGAAAAGAAAAGTTGTGTTGGTTAAAGGGGGATCTAAAACAAAGCATTTCCATAAGAAGGCTGCTAATCGAGATCCATTATTTTATGATACGCTGGGAGAGGAAGGAAGGGAAGGAGGAGGAGTCAGAGAAGATGAGGAACAATTGAAGGAGAAAAAGAAGAGAAAGAAGCTCGAATACACTGTATGTGAAGCAGAACCTGAGCAGCCGGTTCGACATTACCCACTGTGGGTGAAGGTAGAAGGTGAAGTAGATCCAGAGCCGGTTTATATTCCAACTCCTTCTGTCATTGAACCTATAAAACCATTGGTGTTGCCTCAGCCGGATTTATCTTCTACTACGAAGGGCAAACTAACCCCTGGAATTAAACCAGCACGAACATATACTCCCAAGCCCAATCCTGTGATACGAGAAGAGGACAAAGATCCAGTCTATTTGTACTTTGAAAGTATGATGACTTGTGCCCGAGTTCGAGTATATGAACGAAAGAAAGAGGAACAGAGACAACTGTCTCCACCCCTGTCTCCATCCTCATCATTTCAGCAGCAGAGTTCCTGTTACTCTAGTCCTGAGAACCGTGTTACAAAGGAACTTGATTCTGAACAGACCTTAAAGCAGCTCATTTGTGACTTGGAGGATGATTCTGATAAATCACAAGTGGGCTTAAAACTGAATTCAGTGGATCCAACAGTGAGCATTGATCTTAAATACTTGGGAGTACAATTGCCTTTGGCTCCAGCCACCAGCTTTCCCCTATGGAATGTTACAGGTACCAACCCTGCCTCACCTGATGCTGGGTTTCCTTTTGTTTCTAGGACGGGGAAGACCAATGACTTTACCAAAATCAAGGGATGGAGAGGAAAATTTCAAAATGCTTCTGCATCTAGGAATGAAGGTGGAAATTCAGAGGCTTCACTGAAAAACCGTTCTGCTTTCTGTAGTGATAAGCTAGATGAGTACTTGGAAAATGAAGGCAAATTGATGGAAACAAACATAGGTTTCTCTTCAAATGCTCCCACATCTCCAGTAGTGTACCAGCTACCCACCAAGAGTACCAGCTATGTTCGAACTCTTGATAGTGTACTAAAGAAGCAATCTACCATTTCCCCTTCTACCTCTCACTCTGTGAAGCCTCAGTCTGTAACCACTGCCTCTCGAAAAACAAAGGCTCAGAACAAACAGACAACACTCAGTGGCCGAACTAAATCATCTTATAAGTCTATTTTACCATACCCTGTTTCACCAAAGCAGAAAAACTCTCATGTGAGCCAAGGAGATAAAATTACCAAGAATTCCTTGAGTTCGACCTCAGATAATCAGGTGACTAACTTGGTTGTGCCATCTGTAGATGAAAATGCATTTCCAAAGCAGATTAGTTTGCGGCAGGCCCAGCAACAGCACCTTCAGCAGCAAGGAACTCGCCCTCCAGGCTTGTCGAAATCTCAAGTAAAGCTTATGGACCTGGAAGACTGTGCACTCTGGGAAGGAAAACCAAGGACCTATATTACTGAAGAGCGAGCAGATGTCTCCTTGACAACTCTGCTTACTGCTCAGGCATCTCTCAAAACTAAACCTATCCACACAATCATAAGGAAACGAGCTCCTCCATGCAACAATGACTTCTGTCGCCTGGGTTGTGTGTGCTCTAGTCTAGCTTTGGAAAAGCGCCAACCTGCTCACTGCCGTCGACCAGACTGCATGTTTGGTTGCACTTGTTTGAAAAGAAAAGTTGTGTTGGTTAAAGGGGGATCTAAAACAAAGCATTTCCATAAGAAGGCTGCTAATCGAGATCCATTATTTTATGATACGCTGGGAGAGGAAGGAAGGGAAGGAGGAGGAGTCAGAGAAGATGAGGAACAATTGAAGGAGAAAAAGAAGAGAAAGAAGCTCGAATACACTGTATGTGAAGCAGAACCTGAGCAGCCGGTTCGACATTACCCACTGTGGGTGAAGGTAGAAGGTGAAGTAGATCCAGAGCCGGTTTATATTCCAACTCCTTCTGTCATTGAACCTATAAAACCATTGGTGTTGCCTCAGCCGGATTTATCTTCTACTACGAAGGGCAAACTAACCCCTGGAATTAAACCAGCACGAACATATACTCCCAAGCCCAATCCTGTGATACGAGAAGAGGACAAAGATCCAGTCTATTTGTACTTTGAAAGTATGATGACTTGTGCCCGAGTTCGAGTATATGAACGAAAGAAAGAGGAACAGAGACAACTGTCTCCACCCCTGTCTCCATCCTCATCATTTCAGCAGCAGAGTTCCTGTTACTCTAGTCCTGAGAACCGTGTTACAAAGGAACTTGATTCTGAACAGACCTTAAAGCAGCTCATTTGTGACTTGGAGGATGATTCTGATAAATCACAA
Peptide sequence:
METNIGFSSNAPTSPVVYQLPTKSTSYVRTLDSVLKKQSTISPSTSHSVKPQSVTTASRKTKAQNKQTTLSGRTKSSYKSILPYPVSPKQKNSHVSQGDKITKNSLSSTSDNQVTNLVVPSVDENAFPKQISLRQAQQQHLQQQGTRPPGLSKSQVKLMDLEDCALWEGKPRTYITEERADVSLTTLLTAQASLKTKPIHTIIRKRAPPCNNDFCRLGCVCSSLALEKRQPAHCRRPDCMFGCTCLKRKVVLVKGGSKTKHFHKKAANRDPLFYDTLGEEGREGGGVREDEEQLKEKKKRKKLEYTVCEAEPEQPVRHYPLWVKVEGEVDPEPVYIPTPSVIEPIKPLVLPQPDLSSTTKGKLTPGIKPARTYTPKPNPVIREEDKDPVYLYFESMMTCARVRVYERKKEEQRQLSPPLSPSSSFQQQSSCYSSPENRVTKELDSEQTLKQLICDLEDDSDKSQVGLKLNSVDPTVSIDLKYLGVQLPLAPATSFPLWNVTGTNPASPDAGFPFVSRTGKTNDFTKIKGWRGKFQNASASRNEGGNSEASLKNRSAFCSDKLDEYLENEGKLMETNIGFSSNAPTSPVVYQLPTKSTSYVRTLDSVLKKQSTISPSTSHSVKPQSVTTASRKTKAQNKQTTLSGRTKSSYKSILPYPVSPKQKNSHVSQGDKITKNSLSSTSDNQVTNLVVPSVDENAFPKQISLRQAQQQHLQQQGTRPPGLSKSQVKLMDLEDCALWEGKPRTYITEERADVSLTTLLTAQASLKTKPIHTIIRKRAPPCNNDFCRLGCVCSSLALEKRQPAHCRRPDCMFGCTCLKRKVVLVKGGSKTKHFHKKAANRDPLFYDTLGEEGREGGGVREDEEQLKEKKKRKKLEYTVCEAEPEQPVRHYPLWVKVEGEVDPEPVYIPTPSVIEPIKPLVLPQPDLSSTTKGKLTPGIKPARTYTPKPNPVIREEDKDPVYLYFESMMTCARVRVYERKKEEQRQLSPPLSPSSSFQQQSSCYSSPENRVTKELDSEQTLKQLICDLEDDSDKSQ
IRESfinder result
IRESfinder score
0.68476664
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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