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mmu_circMff_480
Basic information of circular RNA
PanCircBase ID:
mmu_circMff_480
mm39_Chr. Position ID:
chr1|82706936|82713228|+
Gene name:
Mff
Transcript:
ENSMUST00000078332.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
3,4,5
Exon/intron offsets:
0,1897,6203
Genomic length:
6292
Exon/intron sizes in nt:
221,170,89
Flanking exon/introns:
chr1:82705057-82706936|chr1:82713228-82719538
Splice length:
480
Other database IDs:
circAtlas: mmu-Mff_0001
circAtlas: hsa-MFF_0001, CIRCpedia: HSA_CIRCpedia_153321
Human Ortholog IDs:
Mature spliced Sequence:
GGTGAGTGGGGCAGCATTTCCTTCTCCCACTGCTGCTGAGATGGCAGAAATTAGTCGAATTCAGTATGAAATGGAATACACCGAAGGTATTAGTCAGCGAATGAGGGTCCCGGAAAAATTAAAAGTAGCACCACCAAATGCTGACCTGGAGCAAGAGTTCCAAGATGGAGTTCCAAATGCCAGTGTGATAATGCAAGTCCCAGAGAGGATCGTGGTTACAGGAAATAATGAAGACATTTCATTTTCAAGACCAGCAGATCTTGACCTTATCCAGTCCACTCCCTTTAAACCTCTGGCACTGAAAACACCACCACGCGTGCTCACACTGAGTGAGAGACCACTAGATTTCCTGGATTTAGAAAGACCACTTCCAACTCCTCAAAGCGAAGAGAGCCGAGCAGTTGGCAGGCTAAAAAGAGAGCGTTCTATGAGTGAAAATGCTGTTCGCCAAAATGGACAGTTGGTCAGAAACGATTCCAT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AAAGCGAAGAGAGCCGAGC
60.45
CTCAGCAGCAGTGGGAGAAG
60.39
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAAGCGAAGAGAGCCGAGC
60.45
CATCTCAGCAGCAGTGGGAG
60.46
143
PCR template sequence:
AAAGCGAAGAGAGCCGAGCAGTTGGCAGGCTAAAAAGAGAGCGTTCTATGAGTGAAAATGCTGTTCGCCAAAATGGACAGTTGGTCAGAAACGATTCCATGGTGAGTGGGGCAGCATTTCCTTCTCCCACTGCTGCTGAGATGGCAGAAATTAGTCGAATTCAGTATGAAATGGAATACACCGAAGGTATTAGTCAGCGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-203-3p(1), mmu-miR-705(1), mmu-miR-335-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GAAACGATTCCATGGTGAGTGGGGCA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMFF_480_ORF_1
1.1007
0.150177779
0.982363081
918
ORF sequence:
ATGGCAGAAATTAGTCGAATTCAGTATGAAATGGAATACACCGAAGGTATTAGTCAGCGAATGAGGGTCCCGGAAAAATTAAAAGTAGCACCACCAAATGCTGACCTGGAGCAAGAGTTCCAAGATGGAGTTCCAAATGCCAGTGTGATAATGCAAGTCCCAGAGAGGATCGTGGTTACAGGAAATAATGAAGACATTTCATTTTCAAGACCAGCAGATCTTGACCTTATCCAGTCCACTCCCTTTAAACCTCTGGCACTGAAAACACCACCACGCGTGCTCACACTGAGTGAGAGACCACTAGATTTCCTGGATTTAGAAAGACCACTTCCAACTCCTCAAAGCGAAGAGAGCCGAGCAGTTGGCAGGCTAAAAAGAGAGCGTTCTATGAGTGAAAATGCTGTTCGCCAAAATGGACAGTTGGTCAGAAACGATTCCATGGTGAGTGGGGCAGCATTTCCTTCTCCCACTGCTGCTGAGATGGCAGAAATTAGTCGAATTCAGTATGAAATGGAATACACCGAAGGTATTAGTCAGCGAATGAGGGTCCCGGAAAAATTAAAAGTAGCACCACCAAATGCTGACCTGGAGCAAGAGTTCCAAGATGGAGTTCCAAATGCCAGTGTGATAATGCAAGTCCCAGAGAGGATCGTGGTTACAGGAAATAATGAAGACATTTCATTTTCAAGACCAGCAGATCTTGACCTTATCCAGTCCACTCCCTTTAAACCTCTGGCACTGAAAACACCACCACGCGTGCTCACACTGAGTGAGAGACCACTAGATTTCCTGGATTTAGAAAGACCACTTCCAACTCCTCAAAGCGAAGAGAGCCGAGCAGTTGGCAGGCTAAAAAGAGAGCGTTCTATGAGTGAAAATGCTGTTCGCCAAAATGGACAGTTGGTCAGAAACGATTCC
Peptide sequence:
MAEISRIQYEMEYTEGISQRMRVPEKLKVAPPNADLEQEFQDGVPNASVIMQVPERIVVTGNNEDISFSRPADLDLIQSTPFKPLALKTPPRVLTLSERPLDFLDLERPLPTPQSEESRAVGRLKRERSMSENAVRQNGQLVRNDSMVSGAAFPSPTAAEMAEISRIQYEMEYTEGISQRMRVPEKLKVAPPNADLEQEFQDGVPNASVIMQVPERIVVTGNNEDISFSRPADLDLIQSTPFKPLALKTPPRVLTLSERPLDFLDLERPLPTPQSEESRAVGRLKRERSMSENAVRQNGQLVRNDS
IRESfinder result
IRESfinder score
0.563528571
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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