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mmu_circMeis1_376
Basic information of circular RNA
PanCircBase ID:
mmu_circMeis1_376
mm39_Chr. Position ID:
chr11|18834386|18891804|-
Gene name:
Meis1
Transcript:
ENSMUST00000102878.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
11,10,9,8
Exon/intron offsets:
0,1202,21302,57272
Genomic length:
57418
Exon/intron sizes in nt:
90,63,77,146
Flanking exon/introns:
chr11:18831922-18834386|chr11:18891804-18938267
Splice length:
376
Other database IDs:
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_145972
Human Ortholog IDs:
Mature spliced Sequence:
GTGATGGCTTGGACAACAGTGTAGCTTCCCCCAGCACAGGTGACGATGATGACCCTGATAAGGACAAAAAGCGTCACAAAAAGCGTGGCATCTTTCCCAAAGTAGCCACCAATATCATGAGGGCGTGGCTGTTCCAGCATCTAACACACCCTTACCCTTCTGAAGAACAGAAAAAGCAGTTGGCACAAGATACAGGACTTACCATCCTTCAAGTGAACAATTGGTTTATTAATGCCCGGAGAAGAATAGTGCAGCCCATGATAGACCAGTCCAACCGAGCAGGCAATCAGCCAAGGGACACCTTATAACCCTGATGGACAGCCAATGGGAGGTTTTGTAATGGACGGTCAGCAGCACATGGGCATCAGAGCGCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGACAGCCAATGGGAGGTTT
60.25
CCACGCTTTTTGTGACGCTT
60.25
149
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGACAGCCAATGGGAGGTTT
60.25
ATGCCACGCTTTTTGTGACG
60.32
152
PCR template sequence:
GAGCAGGCAATCAGCCAAGGGACACCTTATAACCCTGATGGACAGCCAATGGGAGGTTTTGTAATGGACGGTCAGCAGCACATGGGCATCAGAGCGCCAGGTGATGGCTTGGACAACAGTGTAGCTTCCCCCAGCACAGGTGACGATGATGACCCTGATAAGGACAAAAAGCGTCACAAAAAGCGTGGCATCTTTCCCAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-1198-5p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
ATCAGAGCGCCAGGTGATGGCTTGGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCMEIS1_376_ORF_1
1.0578
0.210397191
0.541042191
360
ORF sequence:
ATGGGAGGTTTTGTAATGGACGGTCAGCAGCACATGGGCATCAGAGCGCCAGGTGATGGCTTGGACAACAGTGTAGCTTCCCCCAGCACAGGTGACGATGATGACCCTGATAAGGACAAAAAGCGTCACAAAAAGCGTGGCATCTTTCCCAAAGTAGCCACCAATATCATGAGGGCGTGGCTGTTCCAGCATCTAACACACCCTTACCCTTCTGAAGAACAGAAAAAGCAGTTGGCACAAGATACAGGACTTACCATCCTTCAAGTGAACAATTGGTTTATTAATGCCCGGAGAAGAATAGTGCAGCCCATGATAGACCAGTCCAACCGAGCAGGCAATCAGCCAAGGGACACCTTATAA
Peptide sequence:
MGGFVMDGQQHMGIRAPGDGLDNSVASPSTGDDDDPDKDKKRHKKRGIFPKVATNIMRAWLFQHLTHPYPSEEQKKQLAQDTGLTILQVNNWFINARRRIVQPMIDQSNRAGNQPRDTL
IRESfinder result
IRESfinder score
0.412529496
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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